(Linear) (Six-base) MAPSORT of: KIEE6350.seq  Check: 5639  from: 1  to: 1053



KIEE6350.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:30  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     568    1053 
   Size:        568     485
 
AflII C'TTAA_G

Cuts at:      0     634    1053 
   Size:        634     419
 
AflIII A'CryG_T

Cuts at:      0     243     245     415    1053 
   Size:        243       2     170     638

  Fragments arranged by size: 

                 638     243     170       2
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     558    1053 
   Size:        558     495
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     122     154    1053 
   Size:        122      32     899

  Fragments arranged by size: 

                 899     122      32
 
ApoI r'AATT_y

Cuts at:      0     384     407     623     824     998    1053 
   Size:        384      23     216     201     174      55

  Fragments arranged by size: 

                 384     216     201     174      55      23
 
AseI AT'TA_AT

Cuts at:      0     896    1053 
   Size:        896     157
 
AvaI C'yCGr_G

Cuts at:      0      47      71    1053 
   Size:         47      24     982

  Fragments arranged by size: 

                 982      47      24
 
BanI G'GyrC_C

Cuts at:      0     114    1053 
   Size:        114     939
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     454    1053 
   Size:        454     599
 
Bme1580I G_kGCm'C

Cuts at:      0     119    1053 
   Size:        119     934
 
BmgBI CAC'GTC

Cuts at:      0     838    1053 
   Size:        838     215
 
BsaAI yAC'GTr

Cuts at:      0     246    1053 
   Size:        246     807
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      76     406    1053 
   Size:         76     330     647

  Fragments arranged by size: 

                 647     330      76
 
BseYI C'CCAG_C

Cuts at:      0      21    1053 
   Size:         21    1032
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     332     860    1053 
   Size:        332     528     193

  Fragments arranged by size: 

                 528     332     193
 
BsmI GAATG_Cn'

Cuts at:      0     649    1053 
   Size:        649     404
 
Bsp1286I G_dGCh'C

Cuts at:      0     119    1053 
   Size:        119     934
 
BsrDI GCAATG_nn'

Cuts at:      0    1002    1053 
   Size:       1002      51
 
BsrFI r'CCGG_y

Cuts at:      0      55    1053 
   Size:         55     998
 
BsrGI T'GTAC_A

Cuts at:      0     742    1053 
   Size:        742     311
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     267    1053 
   Size:        267     786
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     307    1053 
   Size:        307     746
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     634     753    1053 
   Size:        634     119     300

  Fragments arranged by size: 

                 634     300     119
 
BtsI GCAGTG_nn'

Cuts at:      0     344    1053 
   Size:        344     709
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      57      87    1053 
   Size:         57      30     966

  Fragments arranged by size: 

                 966      57      30
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     568    1053 
   Size:        568     485
 
EcoO109I rG'GnC_Cy

Cuts at:      0     730    1053 
   Size:        730     323
 
EcoRI G'AATT_C

Cuts at:      0     998    1053 
   Size:        998      55
 
EcoRV GAT'ATC

Cuts at:      0     529    1053 
   Size:        529     524
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     108    1053 
   Size:        108     945
 
MslI CAynn'nnrTG

Cuts at:      0     300     755    1053 
   Size:        300     455     298

  Fragments arranged by size: 

                 455     300     298
 
NaeI GCC'GGC

Cuts at:      0      57    1053 
   Size:         57     996
 
NgoMIV G'CCGG_C

Cuts at:      0      55    1053 
   Size:         55     998
 
NspI r_CATG'y

Cuts at:      0     419    1053 
   Size:        419     634
 
PciI A'CATG_T

Cuts at:      0     415    1053 
   Size:        415     638
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     916    1053 
   Size:        916     137
 
PfoI T'CCnGG_A

Cuts at:      0     909    1053 
   Size:        909     144
 
PmlI CAC'GTG

Cuts at:      0     246    1053 
   Size:        246     807
 
PpuMI rG'GwC_Cy

Cuts at:      0     730    1053 
   Size:        730     323
 
PsiI TTA'TAA

Cuts at:      0     557    1053 
   Size:        557     496
 
PstI C_TGCA'G

Cuts at:      0     970    1053 
   Size:        970      83
 
SfcI C'TryA_G

Cuts at:      0     966    1053 
   Size:        966      87
 
SmaI CCC'GGG

Cuts at:      0      49    1053 
   Size:         49    1004
 
SmlI C'TyrA_G

Cuts at:      0     634    1053 
   Size:        634     419
 
SspI AAT'ATT

Cuts at:      0     561    1053 
   Size:        561     492
 
StuI AGG'CCT

Cuts at:      0       7    1053 
   Size:          7    1046
 
StyI C'CwwG_G

Cuts at:      0      15    1053 
   Size:         15    1038
 
TatI w'GTAC_w

Cuts at:      0     742    1053 
   Size:        742     311
 
XbaI T'CTAG_A

Cuts at:      0     714    1053 
   Size:        714     339
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     299    1053 
   Size:        299     754
 
XmaI C'CCGG_G

Cuts at:      0      47    1053 
   Size:         47    1006
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AlfI     AloI     ApoI     AseI     AvaI
     BanI     BbsI Bme1580I    BmgBI    BsaAI    BseRI    BseYI     BsgI
     BsmI Bsp1286I    BsrDI    BsrFI    BsrGI   BstAPI    BstXI    BtgZI
     BtsI     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     MmeI     MslI
     NaeI   NgoMIV     NspI     PciI    PflMI     PfoI     PmlI    PpuMI
     PsiI     PstI     SfcI     SmaI     SmlI     SspI     StuI     StyI
     TatI     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI    AlwNI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI
    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI   BssHII    BssSI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI    EcoNI
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PmeI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SwaI    TaqII     TstI  Tth111I     XhoI     XmnI     ZraI