Restriction map for
pFN21AE6349
(Linear) (Six-base) MAPSORT of: KIEE6349.seq Check: 5406 from: 1 to: 1170
KIEE6349.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 28 1170
Size: 28 1142
Acc65I G'GTAC_C
Cuts at: 0 308 1170
Size: 308 862
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 1170
Size: 878 292
AhdI GACnn_n'nnGTC
Cuts at: 0 24 1170
Size: 24 1146
AlwNI CAG_nnn'CTG
Cuts at: 0 930 1170
Size: 930 240
ApaI G_GGCC'C
Cuts at: 0 852 1126 1170
Size: 852 274 44
Fragments arranged by size:
852 274 44
ApoI r'AATT_y
Cuts at: 0 133 287 367 1050 1170
Size: 133 154 80 683 120
Fragments arranged by size:
683 154 133 120 80
BanI G'GyrC_C
Cuts at: 0 308 1170
Size: 308 862
BanII G_rGCy'C
Cuts at: 0 852 1126 1170
Size: 852 274 44
Fragments arranged by size:
852 274 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 884 1170
Size: 884 286
BciVI GTATCCnnnnn_n'
Cuts at: 0 749 1170
Size: 749 421
BglII A'GATC_T
Cuts at: 0 606 917 1170
Size: 606 311 253
Fragments arranged by size:
606 311 253
Bme1580I G_kGCm'C
Cuts at: 0 812 852 1126 1170
Size: 812 40 274 44
Fragments arranged by size:
812 274 44 40
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 887 940 1170
Size: 46 841 53 230
Fragments arranged by size:
841 230 53 46
BsaI GGTCTCn'nnnn_
Cuts at: 0 1021 1170
Size: 1021 149
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1079 1170
Size: 1079 91
BsmI GAATG_Cn'
Cuts at: 0 773 1170
Size: 773 397
Bsp1286I G_dGCh'C
Cuts at: 0 812 852 1126 1170
Size: 812 40 274 44
Fragments arranged by size:
812 274 44 40
BspHI T'CATG_A
Cuts at: 0 477 1170
Size: 477 693
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 28 957 1170
Size: 28 929 213
Fragments arranged by size:
929 213 28
BsrFI r'CCGG_y
Cuts at: 0 116 1170
Size: 116 1054
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1112 1170
Size: 1112 58
BstYI r'GATC_y
Cuts at: 0 70 431 606 917 1170
Size: 70 361 175 311 253
Fragments arranged by size:
361 311 253 175 70
BtgI C'CryG_G
Cuts at: 0 14 642 842 1170
Size: 14 628 200 328
Fragments arranged by size:
628 328 200 14
DraI TTT'AAA
Cuts at: 0 1055 1170
Size: 1055 115
EaeI y'GGCC_r
Cuts at: 0 672 1170
Size: 672 498
EarI CTCTTCn'nnn_
Cuts at: 0 1012 1170
Size: 1012 158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 878 887 940 1170
Size: 46 832 9 53 230
Fragments arranged by size:
832 230 53 46 9
EcoO109I rG'GnC_Cy
Cuts at: 0 848 849 1122 1123 1170
Size: 848 1 273 1 47
Fragments arranged by size:
848 273 47 1 1
EcoRI G'AATT_C
Cuts at: 0 367 1170
Size: 367 803
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 929 961 1058 1090 1170
Size: 10 32 887 32 97 32 80
Fragments arranged by size:
887 97 80 32 32 32 10
KpnI G_GTAC'C
Cuts at: 0 312 1170
Size: 312 858
MscI TGG'CCA
Cuts at: 0 674 1170
Size: 674 496
NcoI C'CATG_G
Cuts at: 0 14 642 1170
Size: 14 628 528
Fragments arranged by size:
628 528 14
NdeI CA'TA_TG
Cuts at: 0 804 1170
Size: 804 366
PflMI CCAn_nnn'nTGG
Cuts at: 0 339 999 1076 1170
Size: 339 660 77 94
Fragments arranged by size:
660 339 94 77
PfoI T'CCnGG_A
Cuts at: 0 956 1170
Size: 956 214
PspOMI G'GGCC_C
Cuts at: 0 848 1122 1170
Size: 848 274 48
Fragments arranged by size:
848 274 48
PstI C_TGCA'G
Cuts at: 0 558 834 1170
Size: 558 276 336
Fragments arranged by size:
558 336 276
SfcI C'TryA_G
Cuts at: 0 554 830 1170
Size: 554 276 340
Fragments arranged by size:
554 340 276
SspI AAT'ATT
Cuts at: 0 150 1170
Size: 150 1020
StyI C'CwwG_G
Cuts at: 0 14 521 642 1031 1158 1170
Size: 14 507 121 389 127 12
Fragments arranged by size:
507 389 127 121 14 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 987 1170
Size: 987 183
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 409 1170
Size: 409 761
XmnI GAAnn'nnTTC
Cuts at: 0 494 505 530 1170
Size: 494 11 25 640
Fragments arranged by size:
640 494 25 11
Enzymes that do cut:
AarI Acc65I AcuI AhdI AlwNI ApaI ApoI BanI
BanII BbsI BciVI BglII Bme1580I BpmI BsaI BseRI
BsmI Bsp1286I BspHI BspMI BsrFI BstAPI BstYI BtgI
DraI EaeI EarI Eco57MI EcoO109I EcoRI Hin4I KpnI
MscI NcoI NdeI PflMI PfoI PspOMI PstI SfcI
SspI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglI
BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI BspEI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI MmeI MslI MspA1I NaeI NarI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI
TatI TstI Tth111I XbaI XhoI XmaI ZraI