Restriction map for
pFN21AE6348
(Linear) (Six-base) MAPSORT of: KIEE6348.seq Check: 6302 from: 1 to: 993
KIEE6348.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 522 993
Size: 522 471
AatII G_ACGT'C
Cuts at: 0 292 993
Size: 292 701
AccI GT'mk_AC
Cuts at: 0 500 993
Size: 500 493
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 316 993
Size: 277 39 677
Fragments arranged by size:
677 277 39
AleI CACnn'nnGTG
Cuts at: 0 358 993
Size: 358 635
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 980 993
Size: 980 13
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 391 423 993
Size: 391 32 570
Fragments arranged by size:
570 391 32
AlwNI CAG_nnn'CTG
Cuts at: 0 206 993
Size: 206 787
ApaI G_GGCC'C
Cuts at: 0 24 917 993
Size: 24 893 76
Fragments arranged by size:
893 76 24
AvaI C'yCGr_G
Cuts at: 0 314 993
Size: 314 679
BamHI G'GATC_C
Cuts at: 0 485 993
Size: 485 508
BanI G'GyrC_C
Cuts at: 0 43 59 769 835 993
Size: 43 16 710 66 158
Fragments arranged by size:
710 158 66 43 16
BanII G_rGCy'C
Cuts at: 0 24 188 548 917 993
Size: 24 164 360 369 76
Fragments arranged by size:
369 360 164 76 24
BbeI G_GCGC'C
Cuts at: 0 47 63 773 993
Size: 47 16 710 220
Fragments arranged by size:
710 220 47 16
BglI GCCn_nnn'nGGC
Cuts at: 0 757 993
Size: 757 236
BglII A'GATC_T
Cuts at: 0 455 993
Size: 455 538
Bme1580I G_kGCm'C
Cuts at: 0 24 840 917 993
Size: 24 816 77 76
Fragments arranged by size:
816 77 76 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 340 993
Size: 340 653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 304 895 904 940 993
Size: 127 177 591 9 36 53
Fragments arranged by size:
591 177 127 53 36 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 157 993
Size: 157 836
BsaHI Gr'CG_yC
Cuts at: 0 44 60 289 770 938 993
Size: 44 16 229 481 168 55
Fragments arranged by size:
481 229 168 55 44 16
BsaWI w'CCGG_w
Cuts at: 0 272 803 993
Size: 272 531 190
Fragments arranged by size:
531 272 190
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 993
Size: 391 30 572
Fragments arranged by size:
572 391 30
BseYI C'CCAG_C
Cuts at: 0 39 192 601 781 993
Size: 39 153 409 180 212
Fragments arranged by size:
409 212 180 153 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 993
Size: 376 617
BsiHKAI G_wGCw'C
Cuts at: 0 548 719 993
Size: 548 171 274
Fragments arranged by size:
548 274 171
BsmBI CGTCTCn'nnnn_
Cuts at: 0 280 993
Size: 280 713
Bsp1286I G_dGCh'C
Cuts at: 0 24 188 548 719 840 917 993
Size: 24 164 360 171 121 77 76
Fragments arranged by size:
360 171 164 121 77 76 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 522 993
Size: 341 181 471
Fragments arranged by size:
471 341 181
BsrDI GCAATG_nn'
Cuts at: 0 731 993
Size: 731 262
BsrFI r'CCGG_y
Cuts at: 0 616 924 993
Size: 616 308 69
Fragments arranged by size:
616 308 69
BsrGI T'GTAC_A
Cuts at: 0 143 512 692 993
Size: 143 369 180 301
Fragments arranged by size:
369 301 180 143
BssHII G'CGCG_C
Cuts at: 0 96 993
Size: 96 897
BstAPI GCAn_nnn'nTGC
Cuts at: 0 206 993
Size: 206 787
BstXI CCAn_nnnn'nTGG
Cuts at: 0 39 54 993
Size: 39 15 939
Fragments arranged by size:
939 39 15
BstYI r'GATC_y
Cuts at: 0 455 485 993
Size: 455 30 508
Fragments arranged by size:
508 455 30
Bsu36I CC'TnA_GG
Cuts at: 0 559 993
Size: 559 434
BtgI C'CryG_G
Cuts at: 0 32 47 656 840 993
Size: 32 15 609 184 153
Fragments arranged by size:
609 184 153 32 15
BtsI GCAGTG_nn'
Cuts at: 0 313 370 993
Size: 313 57 623
Fragments arranged by size:
623 313 57
DraIII CAC_nnn'GTG
Cuts at: 0 376 993
Size: 376 617
EaeI y'GGCC_r
Cuts at: 0 25 114 449 794 993
Size: 25 89 335 345 199
Fragments arranged by size:
345 335 199 89 25
EarI CTCTTCn'nnn_
Cuts at: 0 331 359 553 993
Size: 331 28 194 440
Fragments arranged by size:
440 331 194 28
EcoICRI GAG'CTC
Cuts at: 0 546 993
Size: 546 447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 277 304 316 895 904 940 993
Size: 127 150 27 12 579 9 36 53
Fragments arranged by size:
579 150 127 53 36 27 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 558 993
Size: 558 435
EcoO109I rG'GnC_Cy
Cuts at: 0 606 750 913 914 993
Size: 606 144 163 1 79
Fragments arranged by size:
606 163 144 79 1
HaeII r_GCGC'y
Cuts at: 0 47 63 86 773 779 993
Size: 47 16 23 687 6 214
Fragments arranged by size:
687 214 47 23 16 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 993
Size: 142 32 819
Fragments arranged by size:
819 142 32
KasI G'GCGC_C
Cuts at: 0 43 59 769 993
Size: 43 16 710 224
Fragments arranged by size:
710 224 43 16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 993
Size: 452 541
MslI CAynn'nnrTG
Cuts at: 0 52 358 993
Size: 52 306 635
Fragments arranged by size:
635 306 52
MspA1I CmG'CkG
Cuts at: 0 12 282 390 828 882 901 993
Size: 12 270 108 438 54 19 92
Fragments arranged by size:
438 270 108 92 54 19 12
NaeI GCC'GGC
Cuts at: 0 926 993
Size: 926 67
NarI GG'CG_CC
Cuts at: 0 44 60 770 993
Size: 44 16 710 223
Fragments arranged by size:
710 223 44 16
NcoI C'CATG_G
Cuts at: 0 47 993
Size: 47 946
NdeI CA'TA_TG
Cuts at: 0 338 993
Size: 338 655
NgoMIV G'CCGG_C
Cuts at: 0 924 993
Size: 924 69
NspI r_CATG'y
Cuts at: 0 513 867 993
Size: 513 354 126
Fragments arranged by size:
513 354 126
PflMI CCAn_nnn'nTGG
Cuts at: 0 244 993
Size: 244 749
PfoI T'CCnGG_A
Cuts at: 0 104 993
Size: 104 889
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 391 423 993
Size: 391 32 570
Fragments arranged by size:
570 391 32
PspOMI G'GGCC_C
Cuts at: 0 20 913 993
Size: 20 893 80
Fragments arranged by size:
893 80 20
PstI C_TGCA'G
Cuts at: 0 211 308 758 833 993
Size: 211 97 450 75 160
Fragments arranged by size:
450 211 160 97 75
PvuII CAG'CTG
Cuts at: 0 12 282 390 828 882 993
Size: 12 270 108 438 54 111
Fragments arranged by size:
438 270 111 108 54 12
SacI G_AGCT'C
Cuts at: 0 548 993
Size: 548 445
SapI GCTCTTCn'nnn_
Cuts at: 0 553 993
Size: 553 440
SbfI CC_TGCA'GG
Cuts at: 0 758 993
Size: 758 235
SfcI C'TryA_G
Cuts at: 0 207 304 754 829 993
Size: 207 97 450 75 164
Fragments arranged by size:
450 207 164 97 75
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 757 993
Size: 757 236
SfoI GGC'GCC
Cuts at: 0 45 61 771 993
Size: 45 16 710 222
Fragments arranged by size:
710 222 45 16
StyI C'CwwG_G
Cuts at: 0 47 171 227 993
Size: 47 124 56 766
Fragments arranged by size:
766 124 56 47
TatI w'GTAC_w
Cuts at: 0 143 512 623 692 993
Size: 143 369 111 69 301
Fragments arranged by size:
369 301 143 111 69
Tth111I GACn'n_nGTC
Cuts at: 0 850 993
Size: 850 143
ZraI GAC'GTC
Cuts at: 0 290 993
Size: 290 703
Enzymes that do cut:
AarI AatII AccI AcuI AleI AlfI AloI AlwNI
ApaI AvaI BamHI BanI BanII BbeI BglI BglII
Bme1580I BplI BpmI BsaI BsaHI BsaWI BsaXI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI
BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI MmeI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PflMI PfoI PpiI PspOMI PstI PvuII
SacI SapI SbfI SfcI SfiI SfoI StyI TatI
Tth111I ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI
BcgI BciVI BclI BfrBI BlpI BmgBI BmrI BmtI
Bpu10I BpuEI BsaAI BsaBI BseRI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MscI NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI XbaI XcmI XhoI
XmaI XmnI