Restriction map for
pFN21AE6347
(Linear) (Six-base) MAPSORT of: KIEE6347.seq Check: 522 from: 1 to: 981
KIEE6347.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AatII G_ACGT'C
Cuts at: 0 125 981
Size: 125 856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 475 644 709 753 898 981
Size: 136 339 169 65 44 145 83
Fragments arranged by size:
339 169 145 136 83 65 44
AlwNI CAG_nnn'CTG
Cuts at: 0 60 636 981
Size: 60 576 345
Fragments arranged by size:
576 345 60
ApaI G_GGCC'C
Cuts at: 0 591 981
Size: 591 390
AvaI C'yCGr_G
Cuts at: 0 96 183 815 981
Size: 96 87 632 166
Fragments arranged by size:
632 166 96 87
BanI G'GyrC_C
Cuts at: 0 153 932 981
Size: 153 779 49
Fragments arranged by size:
779 153 49
BanII G_rGCy'C
Cuts at: 0 591 837 981
Size: 591 246 144
Fragments arranged by size:
591 246 144
BbeI G_GCGC'C
Cuts at: 0 157 981
Size: 157 824
BbsI GAAGACnn'nnnn_
Cuts at: 0 781 981
Size: 781 200
BciVI GTATCCnnnnn_n'
Cuts at: 0 181 981
Size: 181 800
BclI T'GATC_A
Cuts at: 0 737 981
Size: 737 244
BlpI GC'TnA_GC
Cuts at: 0 467 981
Size: 467 514
Bme1580I G_kGCm'C
Cuts at: 0 591 981
Size: 591 390
BmtI G_CTAG'C
Cuts at: 0 893 981
Size: 893 88
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 981
Size: 161 820
BsaI GGTCTCn'nnnn_
Cuts at: 0 137 981
Size: 137 844
BsaHI Gr'CG_yC
Cuts at: 0 44 122 154 981
Size: 44 78 32 827
Fragments arranged by size:
827 78 44 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 134 164 981
Size: 134 30 817
Fragments arranged by size:
817 134 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 230 326 981
Size: 230 96 655
Fragments arranged by size:
655 230 96
BseYI C'CCAG_C
Cuts at: 0 288 360 981
Size: 288 72 621
Fragments arranged by size:
621 288 72
BsiHKAI G_wGCw'C
Cuts at: 0 459 981
Size: 459 522
BsmI GAATG_Cn'
Cuts at: 0 759 981
Size: 759 222
Bsp1286I G_dGCh'C
Cuts at: 0 459 591 837 981
Size: 459 132 246 144
Fragments arranged by size:
459 246 144 132
BspHI T'CATG_A
Cuts at: 0 858 981
Size: 858 123
BsrBI CCG'CTC
Cuts at: 0 69 981
Size: 69 912
BsrFI r'CCGG_y
Cuts at: 0 605 981
Size: 605 376
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 971 981
Size: 971 10
DraIII CAC_nnn'GTG
Cuts at: 0 639 981
Size: 639 342
DrdI GACnn_nn'nnGTC
Cuts at: 0 785 981
Size: 785 196
EaeI y'GGCC_r
Cuts at: 0 51 320 981
Size: 51 269 661
Fragments arranged by size:
661 269 51
EarI CTCTTCn'nnn_
Cuts at: 0 620 772 981
Size: 620 152 209
Fragments arranged by size:
620 209 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 535 981
Size: 535 446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 161 475 644 709 753 898 981
Size: 136 25 314 169 65 44 145 83
Fragments arranged by size:
314 169 145 136 83 65 44 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 640 981
Size: 640 341
EcoO109I rG'GnC_Cy
Cuts at: 0 498 587 588 762 981
Size: 498 89 1 174 219
Fragments arranged by size:
498 219 174 89 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 783 981
Size: 783 198
HaeII r_GCGC'y
Cuts at: 0 157 981
Size: 157 824
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 789 821 981
Size: 789 32 160
Fragments arranged by size:
789 160 32
HindIII A'AGCT_T
Cuts at: 0 652 981
Size: 652 329
KasI G'GCGC_C
Cuts at: 0 153 981
Size: 153 828
MscI TGG'CCA
Cuts at: 0 322 981
Size: 322 659
MspA1I CmG'CkG
Cuts at: 0 466 981
Size: 466 515
NaeI GCC'GGC
Cuts at: 0 607 981
Size: 607 374
NarI GG'CG_CC
Cuts at: 0 154 981
Size: 154 827
NgoMIV G'CCGG_C
Cuts at: 0 605 981
Size: 605 376
NheI G'CTAG_C
Cuts at: 0 889 981
Size: 889 92
NspI r_CATG'y
Cuts at: 0 306 942 981
Size: 306 636 39
Fragments arranged by size:
636 306 39
PfoI T'CCnGG_A
Cuts at: 0 29 425 981
Size: 29 396 556
Fragments arranged by size:
556 396 29
PpuMI rG'GwC_Cy
Cuts at: 0 498 981
Size: 498 483
PspOMI G'GGCC_C
Cuts at: 0 587 981
Size: 587 394
PvuII CAG'CTG
Cuts at: 0 466 981
Size: 466 515
SapI GCTCTTCn'nnn_
Cuts at: 0 620 981
Size: 620 361
SexAI A'CCwGG_T
Cuts at: 0 635 981
Size: 635 346
SfcI C'TryA_G
Cuts at: 0 965 981
Size: 965 16
SfoI GGC'GCC
Cuts at: 0 155 981
Size: 155 826
SgrAI Cr'CCGG_yG
Cuts at: 0 605 981
Size: 605 376
SmaI CCC'GGG
Cuts at: 0 817 981
Size: 817 164
SphI G_CATG'C
Cuts at: 0 306 981
Size: 306 675
StyI C'CwwG_G
Cuts at: 0 758 981
Size: 758 223
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 713 981
Size: 713 268
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 134 166 981
Size: 134 32 815
Fragments arranged by size:
815 134 32
XmaI C'CCGG_G
Cuts at: 0 815 981
Size: 815 166
XmnI GAAnn'nnTTC
Cuts at: 0 556 981
Size: 556 425
ZraI GAC'GTC
Cuts at: 0 123 981
Size: 123 858
Enzymes that do cut:
AatII AcuI AlwNI ApaI AvaI BanI BanII BbeI
BbsI BciVI BclI BlpI Bme1580I BmtI BpmI BsaI
BsaHI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI
BsrBI BsrFI BtgZI DraIII DrdI EaeI EarI EciI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KasI
MscI MspA1I NaeI NarI NgoMIV NheI NspI PfoI
PpuMI PspOMI PvuII SapI SexAI SfcI SfoI SgrAI
SmaI SphI StyI TaqII TstI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BfrBI BglI
BglII BmgBI BmrI BplI Bpu10I BpuEI BsaAI BsaBI
BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI EagI
EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HpaI
KpnI MfeI MluI MmeI MslI NcoI NdeI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SfiI SgfI
SmlI SnaBI SpeI SrfI SspI StuI SwaI TatI
Tth111I XbaI XcmI XhoI