Restriction map for
pFN21AE6343
(Linear) (Six-base) MAPSORT of: KIEE6343.seq Check: 1472 from: 1 to: 933
KIEE6343.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
Acc65I G'GTAC_C
Cuts at: 0 665 933
Size: 665 268
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 561 933
Size: 399 162 372
Fragments arranged by size:
399 372 162
AfeI AGC'GCT
Cuts at: 0 99 933
Size: 99 834
AleI CACnn'nnGTG
Cuts at: 0 340 933
Size: 340 593
AlwNI CAG_nnn'CTG
Cuts at: 0 27 584 625 933
Size: 27 557 41 308
Fragments arranged by size:
557 308 41 27
AvaI C'yCGr_G
Cuts at: 0 211 870 933
Size: 211 659 63
Fragments arranged by size:
659 211 63
BanI G'GyrC_C
Cuts at: 0 61 367 642 665 933
Size: 61 306 275 23 268
Fragments arranged by size:
306 275 268 61 23
BanII G_rGCy'C
Cuts at: 0 896 933
Size: 896 37
BbeI G_GCGC'C
Cuts at: 0 65 933
Size: 65 868
BglI GCCn_nnn'nGGC
Cuts at: 0 118 724 933
Size: 118 606 209
Fragments arranged by size:
606 209 118
BglII A'GATC_T
Cuts at: 0 686 933
Size: 686 247
BmgBI CAC'GTC
Cuts at: 0 918 933
Size: 918 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 174 230 641 933
Size: 174 56 411 292
Fragments arranged by size:
411 292 174 56
Bpu10I CC'TnA_GC
Cuts at: 0 890 933
Size: 890 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 454 933
Size: 277 177 479
Fragments arranged by size:
479 277 177
BsaAI yAC'GTr
Cuts at: 0 338 342 933
Size: 338 4 591
Fragments arranged by size:
591 338 4
BsaBI GATnn'nnATC
Cuts at: 0 691 933
Size: 691 242
BsaHI Gr'CG_yC
Cuts at: 0 62 183 933
Size: 62 121 750
Fragments arranged by size:
750 121 62
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 780 933
Size: 780 153
BseYI C'CCAG_C
Cuts at: 0 57 933
Size: 57 876
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 933
Size: 721 212
BsiWI C'GTAC_G
Cuts at: 0 338 933
Size: 338 595
BsmBI CGTCTCn'nnnn_
Cuts at: 0 472 933
Size: 472 461
Bsp1286I G_dGCh'C
Cuts at: 0 896 933
Size: 896 37
BsrBI CCG'CTC
Cuts at: 0 306 933
Size: 306 627
BsrDI GCAATG_nn'
Cuts at: 0 188 933
Size: 188 745
BssHII G'CGCG_C
Cuts at: 0 50 933
Size: 50 883
BstEII G'GTnAC_C
Cuts at: 0 672 933
Size: 672 261
BstYI r'GATC_y
Cuts at: 0 686 933
Size: 686 247
BtsI GCAGTG_nn'
Cuts at: 0 154 531 933
Size: 154 377 402
Fragments arranged by size:
402 377 154
DraIII CAC_nnn'GTG
Cuts at: 0 384 649 933
Size: 384 265 284
Fragments arranged by size:
384 284 265
EarI CTCTTCn'nnn_
Cuts at: 0 418 571 703 763 784 790 933
Size: 418 153 132 60 21 6 143
Fragments arranged by size:
418 153 143 132 60 21 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 561 933
Size: 399 162 372
Fragments arranged by size:
399 372 162
EcoO109I rG'GnC_Cy
Cuts at: 0 622 933
Size: 622 311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 774 933
Size: 774 159
HaeII r_GCGC'y
Cuts at: 0 65 101 756 823 933
Size: 65 36 655 67 110
Fragments arranged by size:
655 110 67 65 36
HincII GTy'rAC
Cuts at: 0 399 933
Size: 399 534
KasI G'GCGC_C
Cuts at: 0 61 933
Size: 61 872
KpnI G_GTAC'C
Cuts at: 0 669 933
Size: 669 264
MslI CAynn'nnrTG
Cuts at: 0 193 340 933
Size: 193 147 593
Fragments arranged by size:
593 193 147
MspA1I CmG'CkG
Cuts at: 0 57 581 933
Size: 57 524 352
Fragments arranged by size:
524 352 57
NarI GG'CG_CC
Cuts at: 0 62 933
Size: 62 871
NspI r_CATG'y
Cuts at: 0 540 933
Size: 540 393
PasI CC'CwG_GG
Cuts at: 0 896 933
Size: 896 37
PflMI CCAn_nnn'nTGG
Cuts at: 0 384 933
Size: 384 549
PfoI T'CCnGG_A
Cuts at: 0 761 933
Size: 761 172
PstI C_TGCA'G
Cuts at: 0 77 161 933
Size: 77 84 772
Fragments arranged by size:
772 84 77
PvuII CAG'CTG
Cuts at: 0 57 933
Size: 57 876
SapI GCTCTTCn'nnn_
Cuts at: 0 418 933
Size: 418 515
SbfI CC_TGCA'GG
Cuts at: 0 77 933
Size: 77 856
SexAI A'CCwGG_T
Cuts at: 0 380 668 933
Size: 380 288 265
Fragments arranged by size:
380 288 265
SfcI C'TryA_G
Cuts at: 0 73 157 933
Size: 73 84 776
Fragments arranged by size:
776 84 73
SfoI GGC'GCC
Cuts at: 0 63 933
Size: 63 870
SmaI CCC'GGG
Cuts at: 0 872 933
Size: 872 61
SmlI C'TyrA_G
Cuts at: 0 211 292 469 933
Size: 211 81 177 464
Fragments arranged by size:
464 211 177 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 355 376 660 933
Size: 355 21 284 273
Fragments arranged by size:
355 284 273 21
TatI w'GTAC_w
Cuts at: 0 572 656 933
Size: 572 84 277
Fragments arranged by size:
572 277 84
Tth111I GACn'n_nGTC
Cuts at: 0 394 933
Size: 394 539
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 195 378 933
Size: 195 183 555
Fragments arranged by size:
555 195 183
XhoI C'TCGA_G
Cuts at: 0 211 933
Size: 211 722
XmaI C'CCGG_G
Cuts at: 0 870 933
Size: 870 63
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlwNI AvaI BanI BanII
BbeI BglI BglII BmgBI BmrI Bpu10I BpuEI BsaAI
BsaBI BsaHI BseRI BseYI BsgI BsiWI BsmBI Bsp1286I
BsrBI BsrDI BssHII BstEII BstYI BtsI DraIII EarI
Eco57MI EcoO109I FalI HaeII HincII KasI KpnI MslI
MspA1I NarI NspI PasI PflMI PfoI PstI PvuII
SapI SbfI SexAI SfcI SfoI SmaI SmlI TaqII
TatI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BlpI Bme1580I BmtI BplI BpmI BsaI BsaWI
BsaXI BsiEI BsiHKAI BsmI BspEI BspHI BspMI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I
HindIII HpaI MfeI MluI MmeI MscI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI ScaI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TstI XbaI XmnI ZraI