Restriction map for
pFN21AE6342
(Linear) (Six-base) MAPSORT of: KIEE6342.seq Check: 5617 from: 1 to: 1152
KIEE6342.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 906 1152
Size: 906 246
AatII G_ACGT'C
Cuts at: 0 821 1152
Size: 821 331
ApoI r'AATT_y
Cuts at: 0 76 877 1152
Size: 76 801 275
Fragments arranged by size:
801 275 76
AvaI C'yCGr_G
Cuts at: 0 5 1152
Size: 5 1147
BanI G'GyrC_C
Cuts at: 0 892 1152
Size: 892 260
BclI T'GATC_A
Cuts at: 0 479 950 1152
Size: 479 471 202
Fragments arranged by size:
479 471 202
BfrBI ATG'CAT
Cuts at: 0 35 1152
Size: 35 1117
BglI GCCn_nnn'nGGC
Cuts at: 0 1039 1152
Size: 1039 113
BglII A'GATC_T
Cuts at: 0 534 922 1152
Size: 534 388 230
Fragments arranged by size:
534 388 230
BmrI ACTGGGnnnn_n'
Cuts at: 0 690 1152
Size: 690 462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 925 1152
Size: 751 174 227
Fragments arranged by size:
751 227 174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 1152
Size: 598 554
BsaHI Gr'CG_yC
Cuts at: 0 701 818 1152
Size: 701 117 334
Fragments arranged by size:
701 334 117
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1090 1152
Size: 1090 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 1152
Size: 218 934
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 906 1152
Size: 906 246
BsrGI T'GTAC_A
Cuts at: 0 512 1152
Size: 512 640
BstEII G'GTnAC_C
Cuts at: 0 733 1152
Size: 733 419
BstXI CCAn_nnnn'nTGG
Cuts at: 0 903 1152
Size: 903 249
BstYI r'GATC_y
Cuts at: 0 534 922 1152
Size: 534 388 230
Fragments arranged by size:
534 388 230
EaeI y'GGCC_r
Cuts at: 0 213 751 1152
Size: 213 538 401
Fragments arranged by size:
538 401 213
EarI CTCTTCn'nnn_
Cuts at: 0 959 1067 1076 1152
Size: 959 108 9 76
Fragments arranged by size:
959 108 76 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 925 1152
Size: 751 174 227
Fragments arranged by size:
751 227 174
EcoRV GAT'ATC
Cuts at: 0 420 1152
Size: 420 732
HaeII r_GCGC'y
Cuts at: 0 52 760 1152
Size: 52 708 392
Fragments arranged by size:
708 392 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 404 436 550 582 1075 1107 1152
Size: 404 32 114 32 493 32 45
Fragments arranged by size:
493 404 114 45 32 32 32
MfeI C'AATT_G
Cuts at: 0 569 1152
Size: 569 583
MscI TGG'CCA
Cuts at: 0 753 1152
Size: 753 399
MslI CAynn'nnrTG
Cuts at: 0 34 1152
Size: 34 1118
NdeI CA'TA_TG
Cuts at: 0 31 1152
Size: 31 1121
NsiI A_TGCA'T
Cuts at: 0 37 1152
Size: 37 1115
NspI r_CATG'y
Cuts at: 0 39 1152
Size: 39 1113
PshAI GACnn'nnGTC
Cuts at: 0 153 1152
Size: 153 999
PstI C_TGCA'G
Cuts at: 0 345 1152
Size: 345 807
SapI GCTCTTCn'nnn_
Cuts at: 0 1076 1152
Size: 1076 76
SfcI C'TryA_G
Cuts at: 0 341 1152
Size: 341 811
SmaI CCC'GGG
Cuts at: 0 7 1152
Size: 7 1145
SmlI C'TyrA_G
Cuts at: 0 613 1152
Size: 613 539
SphI G_CATG'C
Cuts at: 0 39 1152
Size: 39 1113
SrfI GCCC'GGGC
Cuts at: 0 7 1152
Size: 7 1145
StyI C'CwwG_G
Cuts at: 0 1007 1043 1152
Size: 1007 36 109
Fragments arranged by size:
1007 109 36
TatI w'GTAC_w
Cuts at: 0 281 512 1152
Size: 281 231 640
Fragments arranged by size:
640 281 231
XbaI T'CTAG_A
Cuts at: 0 81 1152
Size: 81 1071
XmaI C'CCGG_G
Cuts at: 0 5 1152
Size: 5 1147
XmnI GAAnn'nnTTC
Cuts at: 0 806 1152
Size: 806 346
ZraI GAC'GTC
Cuts at: 0 819 1152
Size: 819 333
Enzymes that do cut:
AarI AatII ApoI AvaI BanI BclI BfrBI BglI
BglII BmrI BpmI BpuEI BsaHI BseRI BsgI BspMI
BsrGI BstEII BstXI BstYI EaeI EarI Eco57MI EcoRV
HaeII Hin4I MfeI MscI MslI NdeI NsiI NspI
PshAI PstI SapI SfcI SmaI SmlI SphI SrfI
StyI TatI XbaI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI
BbvCI BcgI BciVI BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MluI
MmeI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SspI StuI
SwaI TaqII TstI Tth111I XcmI XhoI