(Linear) (Six-base) MAPSORT of: KIEE6340.seq  Check: 7180  from: 1  to: 1056



KIEE6340.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:30  ..

Acc65I G'GTAC_C

Cuts at:      0     405    1056 
   Size:        405     651
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     930    1014    1056 
   Size:        379     551      84      42

  Fragments arranged by size: 

                 551     379      84      42
 
AlwNI CAG_nnn'CTG

Cuts at:      0      49    1056 
   Size:         49    1007
 
ApoI r'AATT_y

Cuts at:      0     194     215     467    1056 
   Size:        194      21     252     589

  Fragments arranged by size: 

                 589     252     194      21
 
BanI G'GyrC_C

Cuts at:      0     405    1056 
   Size:        405     651
 
BanII G_rGCy'C

Cuts at:      0     348    1056 
   Size:        348     708
 
BclI T'GATC_A

Cuts at:      0     867    1056 
   Size:        867     189
 
BglII A'GATC_T

Cuts at:      0     795    1056 
   Size:        795     261
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     143    1056 
   Size:        143     913
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514    1056 
   Size:        514     542
 
Bpu10I CC'TnA_GC

Cuts at:      0     349    1056 
   Size:        349     707
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     556    1056 
   Size:        458      98     500

  Fragments arranged by size: 

                 500     458      98
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     190     763    1056 
   Size:        190     573     293

  Fragments arranged by size: 

                 573     293     190
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     415    1056 
   Size:        415     641
 
BseYI C'CCAG_C

Cuts at:      0     284     910    1056 
   Size:        284     626     146

  Fragments arranged by size: 

                 626     284     146
 
BsiHKAI G_wGCw'C

Cuts at:      0     846    1056 
   Size:        846     210
 
BsmI GAATG_Cn'

Cuts at:      0     282     609    1056 
   Size:        282     327     447

  Fragments arranged by size: 

                 447     327     282
 
Bsp1286I G_dGCh'C

Cuts at:      0     348     846    1056 
   Size:        348     498     210

  Fragments arranged by size: 

                 498     348     210
 
BspHI T'CATG_A

Cuts at:      0     111    1056 
   Size:        111     945
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      63    1033    1056 
   Size:         63     970      23

  Fragments arranged by size: 

                 970      63      23
 
BsrDI GCAATG_nn'

Cuts at:      0     415    1056 
   Size:        415     641
 
BstYI r'GATC_y

Cuts at:      0     795    1056 
   Size:        795     261
 
Bsu36I CC'TnA_GG

Cuts at:      0     610    1056 
   Size:        610     446
 
BtgI C'CryG_G

Cuts at:      0     128    1056 
   Size:        128     928
 
DraI TTT'AAA

Cuts at:      0     564    1056 
   Size:        564     492
 
DraIII CAC_nnn'GTG

Cuts at:      0      53    1056 
   Size:         53    1003
 
EaeI y'GGCC_r

Cuts at:      0     596    1056 
   Size:        596     460
 
EarI CTCTTCn'nnn_

Cuts at:      0     376    1056 
   Size:        376     680
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     514     930    1014    1056 
   Size:        379     135     416      84      42

  Fragments arranged by size: 

                 416     379     135      84      42
 
EcoO109I rG'GnC_Cy

Cuts at:      0     479    1056 
   Size:        479     577
 
EcoRI G'AATT_C

Cuts at:      0     467    1056 
   Size:        467     589
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     387     575    1056 
   Size:        387     188     481

  Fragments arranged by size: 

                 481     387     188
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     717     749     769     801    1056 
   Size:        717      32      20      32     255

  Fragments arranged by size: 

                 717     255      32      32      20
 
KpnI G_GTAC'C

Cuts at:      0     409    1056 
   Size:        409     647
 
MscI TGG'CCA

Cuts at:      0     598    1056 
   Size:        598     458
 
MslI CAynn'nnrTG

Cuts at:      0      28    1056 
   Size:         28    1028
 
MspA1I CmG'CkG

Cuts at:      0     357    1056 
   Size:        357     699
 
NcoI C'CATG_G

Cuts at:      0     128    1056 
   Size:        128     928
 
PpuMI rG'GwC_Cy

Cuts at:      0     479    1056 
   Size:        479     577
 
PstI C_TGCA'G

Cuts at:      0     854     933    1043    1056 
   Size:        854      79     110      13

  Fragments arranged by size: 

                 854     110      79      13
 
PvuII CAG'CTG

Cuts at:      0     357    1056 
   Size:        357     699
 
SapI GCTCTTCn'nnn_

Cuts at:      0     376    1056 
   Size:        376     680
 
SfcI C'TryA_G

Cuts at:      0     517     850     929    1039    1056 
   Size:        517     333      79     110      17

  Fragments arranged by size: 

                 517     333     110      79      17
 
SmlI C'TyrA_G

Cuts at:      0     473     571    1056 
   Size:        473      98     485

  Fragments arranged by size: 

                 485     473      98
 
StyI C'CwwG_G

Cuts at:      0     128    1056 
   Size:        128     928
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      18     162     298    1056 
   Size:         18     144     136     758

  Fragments arranged by size: 

                 758     144     136      18
 
TatI w'GTAC_w

Cuts at:      0      84    1056 
   Size:         84     972
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     813     845    1056 
   Size:        813      32     211

  Fragments arranged by size: 

                 813     211      32
 
XbaI T'CTAG_A

Cuts at:      0      58     792    1056 
   Size:         58     734     264

  Fragments arranged by size: 

                 734     264      58
 
XmnI GAAnn'nnTTC

Cuts at:      0     254    1056 
   Size:        254     802
 

 Enzymes that do cut:

   Acc65I     AcuI    AlwNI     ApoI     BanI    BanII     BclI    BglII
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BseRI    BseYI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BstYI   Bsu36I     BtgI
     DraI   DraIII     EaeI     EarI  Eco57MI EcoO109I    EcoRI     FalI
    Hin4I     KpnI     MscI     MslI   MspA1I     NcoI    PpuMI     PstI
    PvuII     SapI     SfcI     SmlI     StyI    TaqII     TatI     TstI
     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI
     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI
    CspCI     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XcmI     XhoI
     XmaI     ZraI