Restriction map for
pFN21AE6339
(Linear) (Six-base) MAPSORT of: KIEE6339.seq Check: 9210 from: 1 to: 1287
KIEE6339.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AccI GT'mk_AC
Cuts at: 0 1022 1287
Size: 1022 265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 1082 1213 1287
Size: 334 748 131 74
Fragments arranged by size:
748 334 131 74
AhdI GACnn_n'nnGTC
Cuts at: 0 254 1287
Size: 254 1033
ApoI r'AATT_y
Cuts at: 0 753 1287
Size: 753 534
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 968 1001 1287
Size: 968 33 286
Fragments arranged by size:
968 286 33
BciVI GTATCCnnnnn_n'
Cuts at: 0 388 969 1287
Size: 388 581 318
Fragments arranged by size:
581 388 318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1287
Size: 190 1097
Bpu10I CC'TnA_GC
Cuts at: 0 40 451 1287
Size: 40 411 836
Fragments arranged by size:
836 411 40
BsaI GGTCTCn'nnnn_
Cuts at: 0 1094 1287
Size: 1094 193
BsaAI yAC'GTr
Cuts at: 0 930 1287
Size: 930 357
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 371 401 665 695 712 742 830 860
Size: 371 30 264 30 17 30 88 30
Cuts at: 860 1287
Size: 427
Fragments arranged by size:
427 371 264 88 30 30 30 30
17
BspHI T'CATG_A
Cuts at: 0 371 419 1287
Size: 371 48 868
Fragments arranged by size:
868 371 48
BsrDI GCAATG_nn'
Cuts at: 0 344 398 1287
Size: 344 54 889
Fragments arranged by size:
889 344 54
BsrFI r'CCGG_y
Cuts at: 0 4 1287
Size: 4 1283
BsrGI T'GTAC_A
Cuts at: 0 825 922 1287
Size: 825 97 365
Fragments arranged by size:
825 365 97
BstBI TT'CG_AA
Cuts at: 0 963 1287
Size: 963 324
BstEII G'GTnAC_C
Cuts at: 0 718 1287
Size: 718 569
BtgI C'CryG_G
Cuts at: 0 789 1287
Size: 789 498
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 514 1287
Size: 514 773
BtsI GCAGTG_nn'
Cuts at: 0 815 1287
Size: 815 472
DraI TTT'AAA
Cuts at: 0 68 1079 1287
Size: 68 1011 208
Fragments arranged by size:
1011 208 68
EaeI y'GGCC_r
Cuts at: 0 2 149 1287
Size: 2 147 1138
Fragments arranged by size:
1138 147 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 334 1082 1213 1287
Size: 190 144 748 131 74
Fragments arranged by size:
748 190 144 131 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 764 1287
Size: 764 523
HaeII r_GCGC'y
Cuts at: 0 27 83 458 1287
Size: 27 56 375 829
Fragments arranged by size:
829 375 56 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 240 272 529 561 1266 1287
Size: 240 32 257 32 705 21
Fragments arranged by size:
705 257 240 32 32 21
HincII GTy'rAC
Cuts at: 0 186 341 443 1287
Size: 186 155 102 844
Fragments arranged by size:
844 186 155 102
HpaI GTT'AAC
Cuts at: 0 186 443 1287
Size: 186 257 844
Fragments arranged by size:
844 257 186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 1287
Size: 651 636
MslI CAynn'nnrTG
Cuts at: 0 1016 1152 1287
Size: 1016 136 135
Fragments arranged by size:
1016 136 135
MspA1I CmG'CkG
Cuts at: 0 60 261 667 1287
Size: 60 201 406 620
Fragments arranged by size:
620 406 201 60
NaeI GCC'GGC
Cuts at: 0 6 1287
Size: 6 1281
NcoI C'CATG_G
Cuts at: 0 789 1287
Size: 789 498
NdeI CA'TA_TG
Cuts at: 0 437 623 970 1287
Size: 437 186 347 317
Fragments arranged by size:
437 347 317 186
NgoMIV G'CCGG_C
Cuts at: 0 4 1287
Size: 4 1283
NspI r_CATG'y
Cuts at: 0 832 1209 1287
Size: 832 377 78
Fragments arranged by size:
832 377 78
PfoI T'CCnGG_A
Cuts at: 0 1137 1287
Size: 1137 150
PsiI TTA'TAA
Cuts at: 0 988 1287
Size: 988 299
PstI C_TGCA'G
Cuts at: 0 295 1287
Size: 295 992
PvuII CAG'CTG
Cuts at: 0 261 667 1287
Size: 261 406 620
Fragments arranged by size:
620 406 261
SfcI C'TryA_G
Cuts at: 0 291 1287
Size: 291 996
SnaBI TAC'GTA
Cuts at: 0 930 1287
Size: 930 357
StuI AGG'CCT
Cuts at: 0 180 1287
Size: 180 1107
StyI C'CwwG_G
Cuts at: 0 789 1287
Size: 789 498
SwaI ATTT'AAAT
Cuts at: 0 1079 1287
Size: 1079 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 764 1287
Size: 764 523
TatI w'GTAC_w
Cuts at: 0 359 825 922 1164 1216 1287
Size: 359 466 97 242 52 71
Fragments arranged by size:
466 359 242 97 71 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 712 718 744 750 1287
Size: 712 6 26 6 537
Fragments arranged by size:
712 537 26 6 6
Enzymes that do cut:
AccI AcuI AhdI ApoI BaeI BciVI BpmI Bpu10I
BsaI BsaAI BsaXI BspHI BsrDI BsrFI BsrGI BstBI
BstEII BtgI BtgZI BtsI DraI EaeI Eco57MI EcoNI
HaeII Hin4I HincII HpaI MmeI MslI MspA1I NaeI
NcoI NdeI NgoMIV NspI PfoI PsiI PstI PvuII
SfcI SnaBI StuI StyI SwaI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpuEI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BssHII BssSI BstAPI BstXI
BstYI BstZ17I Bsu36I ClaI CspCI DraIII DrdI EagI
EarI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HindIII KasI KpnI MfeI MluI MscI
NarI NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI SspI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI