Restriction map for
pFN21AE6337
(Linear) (Six-base) MAPSORT of: KIEE6337.seq Check: 4187 from: 1 to: 1155
KIEE6337.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 285 1145 1155
Size: 285 860 10
Fragments arranged by size:
860 285 10
AfeI AGC'GCT
Cuts at: 0 38 1155
Size: 38 1117
AleI CACnn'nnGTG
Cuts at: 0 254 1155
Size: 254 901
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 947 1155
Size: 947 208
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 667 699 1155
Size: 667 32 456
Fragments arranged by size:
667 456 32
AlwNI CAG_nnn'CTG
Cuts at: 0 300 1155
Size: 300 855
ApoI r'AATT_y
Cuts at: 0 730 1155
Size: 730 425
AvaI C'yCGr_G
Cuts at: 0 32 1079 1155
Size: 32 1047 76
Fragments arranged by size:
1047 76 32
BanI G'GyrC_C
Cuts at: 0 916 923 1155
Size: 916 7 232
Fragments arranged by size:
916 232 7
BanII G_rGCy'C
Cuts at: 0 142 1155
Size: 142 1013
BbvCI CC'TCA_GC
Cuts at: 0 394 1155
Size: 394 761
BclI T'GATC_A
Cuts at: 0 482 743 1055 1155
Size: 482 261 312 100
Fragments arranged by size:
482 312 261 100
Bme1580I G_kGCm'C
Cuts at: 0 921 1155
Size: 921 234
BmgBI CAC'GTC
Cuts at: 0 126 1155
Size: 126 1029
BmrI ACTGGGnnnn_n'
Cuts at: 0 223 1155
Size: 223 932
BmtI G_CTAG'C
Cuts at: 0 376 1155
Size: 376 779
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 393 531 1038 1155
Size: 393 138 507 117
Fragments arranged by size:
507 393 138 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 1008 1155
Size: 310 698 147
Fragments arranged by size:
698 310 147
Bpu10I CC'TnA_GC
Cuts at: 0 182 394 1155
Size: 182 212 761
Fragments arranged by size:
761 212 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1155
Size: 1033 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 48 1155
Size: 48 1107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 158 188 851 881 1155
Size: 158 30 663 30 274
Fragments arranged by size:
663 274 158 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 1155
Size: 295 860
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 793 911 1155
Size: 283 510 118 244
Fragments arranged by size:
510 283 244 118
BsiEI CG_ry'CG
Cuts at: 0 89 1155
Size: 89 1066
BsiHKAI G_wGCw'C
Cuts at: 0 248 1155
Size: 248 907
BsmBI CGTCTCn'nnnn_
Cuts at: 0 161 904 1155
Size: 161 743 251
Fragments arranged by size:
743 251 161
Bsp1286I G_dGCh'C
Cuts at: 0 142 248 921 1155
Size: 142 106 673 234
Fragments arranged by size:
673 234 142 106
BspHI T'CATG_A
Cuts at: 0 662 1155
Size: 662 493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 285 1145 1155
Size: 285 860 10
Fragments arranged by size:
860 285 10
BsrBI CCG'CTC
Cuts at: 0 14 1155
Size: 14 1141
BssSI C'ACGA_G
Cuts at: 0 569 1155
Size: 569 586
BstAPI GCAn_nnn'nTGC
Cuts at: 0 771 1155
Size: 771 384
BstEII G'GTnAC_C
Cuts at: 0 1132 1155
Size: 1132 23
BtgI C'CryG_G
Cuts at: 0 89 965 1155
Size: 89 876 190
Fragments arranged by size:
876 190 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 89 1155
Size: 89 1066
BtsI GCAGTG_nn'
Cuts at: 0 638 697 947 1155
Size: 638 59 250 208
Fragments arranged by size:
638 250 208 59
EaeI y'GGCC_r
Cuts at: 0 86 326 968 1155
Size: 86 240 642 187
Fragments arranged by size:
642 240 187 86
EagI C'GGCC_G
Cuts at: 0 86 1155
Size: 86 1069
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 36 1155
Size: 36 1119
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 1008 1155
Size: 310 698 147
Fragments arranged by size:
698 310 147
EcoNI CCTnn'n_nnAGG
Cuts at: 0 256 1155
Size: 256 899
EcoO109I rG'GnC_Cy
Cuts at: 0 178 1155
Size: 178 977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 426 618 1155
Size: 426 192 537
Fragments arranged by size:
537 426 192
HaeII r_GCGC'y
Cuts at: 0 40 1155
Size: 40 1115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 58 795 827 1155
Size: 26 32 737 32 328
Fragments arranged by size:
737 328 32 32 26
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 1013 1155
Size: 386 627 142
Fragments arranged by size:
627 386 142
MscI TGG'CCA
Cuts at: 0 328 1155
Size: 328 827
MslI CAynn'nnrTG
Cuts at: 0 254 530 970 1155
Size: 254 276 440 185
Fragments arranged by size:
440 276 254 185
MspA1I CmG'CkG
Cuts at: 0 23 91 223 585 768 1155
Size: 23 68 132 362 183 387
Fragments arranged by size:
387 362 183 132 68 23
NheI G'CTAG_C
Cuts at: 0 372 1155
Size: 372 783
NspI r_CATG'y
Cuts at: 0 765 996 1155
Size: 765 231 159
Fragments arranged by size:
765 231 159
PasI CC'CwG_GG
Cuts at: 0 58 1155
Size: 58 1097
PflMI CCAn_nnn'nTGG
Cuts at: 0 942 1155
Size: 942 213
PfoI T'CCnGG_A
Cuts at: 0 162 239 1155
Size: 162 77 916
Fragments arranged by size:
916 162 77
PstI C_TGCA'G
Cuts at: 0 692 1142 1155
Size: 692 450 13
Fragments arranged by size:
692 450 13
PvuII CAG'CTG
Cuts at: 0 768 1155
Size: 768 387
SacII CC_GC'GG
Cuts at: 0 92 1155
Size: 92 1063
SexAI A'CCwGG_T
Cuts at: 0 476 1155
Size: 476 679
SfcI C'TryA_G
Cuts at: 0 688 885 1095 1138 1155
Size: 688 197 210 43 17
Fragments arranged by size:
688 210 197 43 17
SmlI C'TyrA_G
Cuts at: 0 1012 1079 1155
Size: 1012 67 76
Fragments arranged by size:
1012 76 67
SphI G_CATG'C
Cuts at: 0 765 1155
Size: 765 390
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 941 1120 1155
Size: 941 179 35
Fragments arranged by size:
941 179 35
TatI w'GTAC_w
Cuts at: 0 509 1155
Size: 509 646
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 158 190 851 883 1155
Size: 158 32 661 32 272
Fragments arranged by size:
661 272 158 32 32
XbaI T'CTAG_A
Cuts at: 0 471 1155
Size: 471 684
XhoI C'TCGA_G
Cuts at: 0 1079 1155
Size: 1079 76
Enzymes that do cut:
AarI AfeI AleI AlfI AloI AlwNI ApoI AvaI
BanI BanII BbvCI BclI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BssSI
BstAPI BstEII BtgI BtgZI BtsI EaeI EagI EciI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MmeI MscI
MslI MspA1I NheI NspI PasI PflMI PfoI PstI
PvuII SacII SexAI SfcI SmlI SphI TaqII TatI
TstI XbaI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BcgI BciVI BfrBI BglI BglII
BlpI BsaAI BsaBI BsaHI BsaWI BseYI BsiWI BsmI
BspEI BsrDI BsrFI BsrGI BssHII BstBI BstXI BstYI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EarI
EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI StyI SwaI Tth111I XcmI XmaI XmnI
ZraI