Restriction map for
pFN21AE6336
(Linear) (Six-base) MAPSORT of: KIEE6336.seq Check: 965 from: 1 to: 1290
KIEE6336.orf
With 182 enzymes: SgfI *
March 16, 2015 11:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 645 777 952 1075 1290
Size: 622 23 132 175 123 215
Fragments arranged by size:
622 215 175 132 123 23
AflIII A'CryG_T
Cuts at: 0 616 618 1234 1290
Size: 616 2 616 56
Fragments arranged by size:
616 616 56 2
AgeI A'CCGG_T
Cuts at: 0 215 1290
Size: 215 1075
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 113 1290
Size: 113 1177
AlwNI CAG_nnn'CTG
Cuts at: 0 563 1065 1290
Size: 563 502 225
Fragments arranged by size:
563 502 225
ApoI r'AATT_y
Cuts at: 0 784 1290
Size: 784 506
AvaI C'yCGr_G
Cuts at: 0 125 1290
Size: 125 1165
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 605 638 1290
Size: 605 33 652
Fragments arranged by size:
652 605 33
BanI G'GyrC_C
Cuts at: 0 893 1290
Size: 893 397
BanII G_rGCy'C
Cuts at: 0 836 1290
Size: 836 454
BbvCI CC'TCA_GC
Cuts at: 0 715 1290
Size: 715 575
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 250 284 1290
Size: 250 34 1006
Fragments arranged by size:
1006 250 34
BglI GCCn_nnn'nGGC
Cuts at: 0 27 48 1138 1290
Size: 27 21 1090 152
Fragments arranged by size:
1090 152 27 21
BlpI GC'TnA_GC
Cuts at: 0 10 872 1290
Size: 10 862 418
Fragments arranged by size:
862 418 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 583 1290
Size: 583 707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 919 1290
Size: 628 291 371
Fragments arranged by size:
628 371 291
Bpu10I CC'TnA_GC
Cuts at: 0 715 925 1290
Size: 715 210 365
Fragments arranged by size:
715 365 210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1290
Size: 820 470
BsaI GGTCTCn'nnnn_
Cuts at: 0 136 739 1290
Size: 136 603 551
Fragments arranged by size:
603 551 136
BsaAI yAC'GTr
Cuts at: 0 619 1237 1290
Size: 619 618 53
Fragments arranged by size:
619 618 53
BsaHI Gr'CG_yC
Cuts at: 0 1091 1290
Size: 1091 199
BsaWI w'CCGG_w
Cuts at: 0 215 1290
Size: 215 1075
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 808 838 1290
Size: 808 30 452
Fragments arranged by size:
808 452 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 877 916 1290
Size: 877 39 374
Fragments arranged by size:
877 374 39
BseYI C'CCAG_C
Cuts at: 0 83 1290
Size: 83 1207
BsiHKAI G_wGCw'C
Cuts at: 0 134 836 1290
Size: 134 702 454
Fragments arranged by size:
702 454 134
Bsp1286I G_dGCh'C
Cuts at: 0 134 836 1290
Size: 134 702 454
Fragments arranged by size:
702 454 134
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 74 314 329 1290
Size: 74 240 15 961
Fragments arranged by size:
961 240 74 15
BsrBI CCG'CTC
Cuts at: 0 685 1290
Size: 685 605
BsrDI GCAATG_nn'
Cuts at: 0 1127 1290
Size: 1127 163
BsrFI r'CCGG_y
Cuts at: 0 215 365 968 1290
Size: 215 150 603 322
Fragments arranged by size:
603 322 215 150
BsrGI T'GTAC_A
Cuts at: 0 824 1290
Size: 824 466
BstAPI GCAn_nnn'nTGC
Cuts at: 0 306 1290
Size: 306 984
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1155 1170 1290
Size: 1155 15 120
Fragments arranged by size:
1155 120 15
BstYI r'GATC_y
Cuts at: 0 110 583 906 1290
Size: 110 473 323 384
Fragments arranged by size:
473 384 323 110
Bsu36I CC'TnA_GG
Cuts at: 0 1217 1290
Size: 1217 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1029 1290
Size: 1029 261
BtsI GCAGTG_nn'
Cuts at: 0 655 725 1290
Size: 655 70 565
Fragments arranged by size:
655 565 70
ClaI AT'CG_AT
Cuts at: 0 266 1290
Size: 266 1024
DraIII CAC_nnn'GTG
Cuts at: 0 1166 1290
Size: 1166 124
EaeI y'GGCC_r
Cuts at: 0 2 335 1290
Size: 2 333 955
Fragments arranged by size:
955 333 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 103 1290
Size: 103 1187
EcoICRI GAG'CTC
Cuts at: 0 834 1290
Size: 834 456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 628 645 777 919 952 1075 1290
Size: 622 6 17 132 142 33 123 215
Fragments arranged by size:
622 215 142 132 123 33 17 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 493 901 1290
Size: 493 408 389
Fragments arranged by size:
493 408 389
EcoO109I rG'GnC_Cy
Cuts at: 0 390 1290
Size: 390 900
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 465 873 1290
Size: 465 408 417
Fragments arranged by size:
465 417 408
FspI TGC'GCA
Cuts at: 0 913 1290
Size: 913 377
HaeII r_GCGC'y
Cuts at: 0 33 1290
Size: 33 1257
HincII GTy'rAC
Cuts at: 0 213 609 1290
Size: 213 396 681
Fragments arranged by size:
681 396 213
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 545 1290
Size: 528 17 745
Fragments arranged by size:
745 528 17
MscI TGG'CCA
Cuts at: 0 4 337 1290
Size: 4 333 953
Fragments arranged by size:
953 333 4
MslI CAynn'nnrTG
Cuts at: 0 379 1290
Size: 379 911
MspA1I CmG'CkG
Cuts at: 0 39 87 1278 1290
Size: 39 48 1191 12
Fragments arranged by size:
1191 48 39 12
NaeI GCC'GGC
Cuts at: 0 367 1290
Size: 367 923
NgoMIV G'CCGG_C
Cuts at: 0 365 1290
Size: 365 925
PmlI CAC'GTG
Cuts at: 0 619 1290
Size: 619 671
PpuMI rG'GwC_Cy
Cuts at: 0 390 1290
Size: 390 900
PshAI GACnn'nnGTC
Cuts at: 0 239 662 1290
Size: 239 423 628
Fragments arranged by size:
628 423 239
PstI C_TGCA'G
Cuts at: 0 92 311 881 1121 1290
Size: 92 219 570 240 169
Fragments arranged by size:
570 240 219 169 92
PvuII CAG'CTG
Cuts at: 0 39 87 1278 1290
Size: 39 48 1191 12
Fragments arranged by size:
1191 48 39 12
SacI G_AGCT'C
Cuts at: 0 836 1290
Size: 836 454
SbfI CC_TGCA'GG
Cuts at: 0 311 1290
Size: 311 979
SfcI C'TryA_G
Cuts at: 0 88 307 877 1117 1290
Size: 88 219 570 240 173
Fragments arranged by size:
570 240 219 173 88
SmaI CCC'GGG
Cuts at: 0 127 1290
Size: 127 1163
SmlI C'TyrA_G
Cuts at: 0 835 1290
Size: 835 455
StuI AGG'CCT
Cuts at: 0 448 472 1290
Size: 448 24 818
Fragments arranged by size:
818 448 24
StyI C'CwwG_G
Cuts at: 0 857 1290
Size: 857 433
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 695 1290
Size: 695 595
TatI w'GTAC_w
Cuts at: 0 824 1290
Size: 824 466
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1081 1113 1150 1182 1242 1274 1290
Size: 1081 32 37 32 60 32 16
Fragments arranged by size:
1081 60 37 32 32 32 16
XmaI C'CCGG_G
Cuts at: 0 125 1290
Size: 125 1165
Enzymes that do cut:
AcuI AflIII AgeI AlfI AlwNI ApoI AvaI BaeI
BanI BanII BbvCI BcgI BglI BlpI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BstAPI BstXI BstYI Bsu36I BtgZI BtsI ClaI DraIII
EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII HincII MmeI MscI MslI MspA1I NaeI NgoMIV
PmlI PpuMI PshAI PstI PvuII SacI SbfI SfcI
SmaI SmlI StuI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BbeI BbsI BciVI BclI BfrBI BglII Bme1580I
BmgBI BmrI BmtI BsaBI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BssHII BssSI BstBI BstEII BstZ17I
BtgI CspCI DraI DrdI EagI EarI EcoRI EcoRV
FseI FspAI Hin4I HindIII HpaI KasI KpnI MfeI
MluI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XcmI XhoI XmnI ZraI