Restriction map for
pFN21AE6333
(Linear) (Six-base) MAPSORT of: KIEE6333.seq Check: 9340 from: 1 to: 960
KIEE6333.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 801 960
Size: 801 159
AatII G_ACGT'C
Cuts at: 0 113 960
Size: 113 847
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 889 960
Size: 118 771 71
Fragments arranged by size:
771 118 71
AflIII A'CryG_T
Cuts at: 0 243 960
Size: 243 717
AlwNI CAG_nnn'CTG
Cuts at: 0 61 207 960
Size: 61 146 753
Fragments arranged by size:
753 146 61
ApaLI G'TGCA_C
Cuts at: 0 358 406 960
Size: 358 48 554
Fragments arranged by size:
554 358 48
ApoI r'AATT_y
Cuts at: 0 349 910 960
Size: 349 561 50
Fragments arranged by size:
561 349 50
AvaI C'yCGr_G
Cuts at: 0 86 344 960
Size: 86 258 616
Fragments arranged by size:
616 258 86
BanI G'GyrC_C
Cuts at: 0 829 960
Size: 829 131
BanII G_rGCy'C
Cuts at: 0 455 960
Size: 455 505
BbeI G_GCGC'C
Cuts at: 0 833 960
Size: 833 127
BbsI GAAGACnn'nnnn_
Cuts at: 0 918 960
Size: 918 42
BclI T'GATC_A
Cuts at: 0 95 960
Size: 95 865
BglI GCCn_nnn'nGGC
Cuts at: 0 391 960
Size: 391 569
Bme1580I G_kGCm'C
Cuts at: 0 362 410 960
Size: 362 48 550
Fragments arranged by size:
550 362 48
BmgBI CAC'GTC
Cuts at: 0 246 960
Size: 246 714
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 960
Size: 172 788
Bpu10I CC'TnA_GC
Cuts at: 0 443 960
Size: 443 517
BsaBI GATnn'nnATC
Cuts at: 0 550 960
Size: 550 410
BsaHI Gr'CG_yC
Cuts at: 0 110 341 830 960
Size: 110 231 489 130
Fragments arranged by size:
489 231 130 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 665 695 960
Size: 665 30 265
Fragments arranged by size:
665 265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 229 960
Size: 229 731
BseYI C'CCAG_C
Cuts at: 0 61 163 249 859 960
Size: 61 102 86 610 101
Fragments arranged by size:
610 102 101 86 61
BsiHKAI G_wGCw'C
Cuts at: 0 362 410 960
Size: 362 48 550
Fragments arranged by size:
550 362 48
Bsp1286I G_dGCh'C
Cuts at: 0 362 410 455 960
Size: 362 48 45 505
Fragments arranged by size:
505 362 48 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 665 801 960
Size: 665 136 159
Fragments arranged by size:
665 159 136
BsrGI T'GTAC_A
Cuts at: 0 605 960
Size: 605 355
BstAPI GCAn_nnn'nTGC
Cuts at: 0 207 960
Size: 207 753
BstYI r'GATC_y
Cuts at: 0 119 960
Size: 119 841
BtgI C'CryG_G
Cuts at: 0 177 413 482 960
Size: 177 236 69 478
Fragments arranged by size:
478 236 177 69
BtsI GCAGTG_nn'
Cuts at: 0 62 162 675 930 960
Size: 62 100 513 255 30
Fragments arranged by size:
513 255 100 62 30
DraIII CAC_nnn'GTG
Cuts at: 0 414 483 960
Size: 414 69 477
Fragments arranged by size:
477 414 69
EaeI y'GGCC_r
Cuts at: 0 392 768 960
Size: 392 376 192
Fragments arranged by size:
392 376 192
EarI CTCTTCn'nnn_
Cuts at: 0 721 960
Size: 721 239
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 172 889 960
Size: 118 54 717 71
Fragments arranged by size:
717 118 71 54
EcoO109I rG'GnC_Cy
Cuts at: 0 508 789 849 960
Size: 508 281 60 111
Fragments arranged by size:
508 281 111 60
EcoRI G'AATT_C
Cuts at: 0 349 910 960
Size: 349 561 50
Fragments arranged by size:
561 349 50
FspI TGC'GCA
Cuts at: 0 427 960
Size: 427 533
HaeII r_GCGC'y
Cuts at: 0 94 147 833 960
Size: 94 53 686 127
Fragments arranged by size:
686 127 94 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 665 697 960
Size: 133 32 500 32 263
Fragments arranged by size:
500 263 133 32 32
KasI G'GCGC_C
Cuts at: 0 829 960
Size: 829 131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 717 960
Size: 247 470 243
Fragments arranged by size:
470 247 243
MscI TGG'CCA
Cuts at: 0 394 770 960
Size: 394 376 190
Fragments arranged by size:
394 376 190
MspA1I CmG'CkG
Cuts at: 0 708 960
Size: 708 252
NarI GG'CG_CC
Cuts at: 0 830 960
Size: 830 130
NcoI C'CATG_G
Cuts at: 0 177 960
Size: 177 783
NspI r_CATG'y
Cuts at: 0 754 960
Size: 754 206
PasI CC'CwG_GG
Cuts at: 0 834 960
Size: 834 126
PflMI CCAn_nnn'nTGG
Cuts at: 0 483 960
Size: 483 477
PstI C_TGCA'G
Cuts at: 0 212 575 960
Size: 212 363 385
Fragments arranged by size:
385 363 212
SfcI C'TryA_G
Cuts at: 0 208 571 585 730 960
Size: 208 363 14 145 230
Fragments arranged by size:
363 230 208 145 14
SfoI GGC'GCC
Cuts at: 0 831 960
Size: 831 129
SmaI CCC'GGG
Cuts at: 0 346 960
Size: 346 614
SphI G_CATG'C
Cuts at: 0 754 960
Size: 754 206
StuI AGG'CCT
Cuts at: 0 193 960
Size: 193 767
StyI C'CwwG_G
Cuts at: 0 177 455 960
Size: 177 278 505
Fragments arranged by size:
505 278 177
TatI w'GTAC_w
Cuts at: 0 605 960
Size: 605 355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 48 960
Size: 48 912
XmaI C'CCGG_G
Cuts at: 0 344 960
Size: 344 616
ZraI GAC'GTC
Cuts at: 0 111 960
Size: 111 849
Enzymes that do cut:
AarI AatII AcuI AflIII AlwNI ApaLI ApoI AvaI
BanI BanII BbeI BbsI BclI BglI Bme1580I BmgBI
BpmI Bpu10I BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrGI BstAPI BstYI BtgI BtsI DraIII
EaeI EarI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
KasI MmeI MscI MspA1I NarI NcoI NspI PasI
PflMI PstI SfcI SfoI SmaI SphI StuI StyI
TatI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BfrBI BglII BlpI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaWI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoNI
EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI MslI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XhoI XmnI