Restriction map for
pFN21AE6332
(Linear) (Six-base) MAPSORT of: KIEE6332.seq Check: 1740 from: 1 to: 1245
KIEE6332.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AccI GT'mk_AC
Cuts at: 0 242 1245
Size: 242 1003
Acc65I G'GTAC_C
Cuts at: 0 954 1245
Size: 954 291
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1245
Size: 277 968
AfeI AGC'GCT
Cuts at: 0 343 1021 1245
Size: 343 678 224
Fragments arranged by size:
678 343 224
AlwNI CAG_nnn'CTG
Cuts at: 0 42 434 1245
Size: 42 392 811
Fragments arranged by size:
811 392 42
ApaI G_GGCC'C
Cuts at: 0 355 1245
Size: 355 890
ApaLI G'TGCA_C
Cuts at: 0 873 1245
Size: 873 372
AseI AT'TA_AT
Cuts at: 0 590 1245
Size: 590 655
AvaI C'yCGr_G
Cuts at: 0 509 583 1245
Size: 509 74 662
Fragments arranged by size:
662 509 74
BanI G'GyrC_C
Cuts at: 0 604 690 754 768 888 954 1002 1245
Size: 604 86 64 14 120 66 48 243
Fragments arranged by size:
604 243 120 86 66 64 48 14
BanII G_rGCy'C
Cuts at: 0 355 795 1245
Size: 355 440 450
Fragments arranged by size:
450 440 355
BbsI GAAGACnn'nnnn_
Cuts at: 0 265 1245
Size: 265 980
BbvCI CC'TCA_GC
Cuts at: 0 634 1245
Size: 634 611
BglI GCCn_nnn'nGGC
Cuts at: 0 574 1245
Size: 574 671
BlpI GC'TnA_GC
Cuts at: 0 424 1245
Size: 424 821
Bme1580I G_kGCm'C
Cuts at: 0 355 478 503 607 695 877 1245
Size: 355 123 25 104 88 182 368
Fragments arranged by size:
368 355 182 123 104 88 25
BmtI G_CTAG'C
Cuts at: 0 227 568 1245
Size: 227 341 677
Fragments arranged by size:
677 341 227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 358 1245
Size: 227 131 887
Fragments arranged by size:
887 227 131
Bpu10I CC'TnA_GC
Cuts at: 0 634 1245
Size: 634 611
BsaAI yAC'GTr
Cuts at: 0 951 987 1245
Size: 951 36 258
Fragments arranged by size:
951 258 36
BsaHI Gr'CG_yC
Cuts at: 0 212 1245
Size: 212 1033
BsaWI w'CCGG_w
Cuts at: 0 20 100 1245
Size: 20 80 1145
Fragments arranged by size:
1145 80 20
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 986 1016 1245
Size: 986 30 229
Fragments arranged by size:
986 229 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 403 1245
Size: 133 270 842
Fragments arranged by size:
842 270 133
BseYI C'CCAG_C
Cuts at: 0 344 578 600 694 908 1245
Size: 344 234 22 94 214 337
Fragments arranged by size:
344 337 234 214 94 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 304 847 1245
Size: 91 213 543 398
Fragments arranged by size:
543 398 213 91
BsiHKAI G_wGCw'C
Cuts at: 0 877 1245
Size: 877 368
Bsp1286I G_dGCh'C
Cuts at: 0 355 478 503 607 695 795 877 1245
Size: 355 123 25 104 88 100 82 368
Fragments arranged by size:
368 355 123 104 100 88 82 25
BspEI T'CCGG_A
Cuts at: 0 100 1245
Size: 100 1145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 323 638 926 1245
Size: 323 315 288 319
Fragments arranged by size:
323 319 315 288
BssHII G'CGCG_C
Cuts at: 0 447 1245
Size: 447 798
BssSI C'ACGA_G
Cuts at: 0 469 647 1245
Size: 469 178 598
Fragments arranged by size:
598 469 178
BstAPI GCAn_nnn'nTGC
Cuts at: 0 434 881 1245
Size: 434 447 364
Fragments arranged by size:
447 434 364
BstBI TT'CG_AA
Cuts at: 0 779 1245
Size: 779 466
BstXI CCAn_nnnn'nTGG
Cuts at: 0 411 1245
Size: 411 834
BstYI r'GATC_y
Cuts at: 0 1181 1245
Size: 1181 64
BtgI C'CryG_G
Cuts at: 0 157 164 266 404 1245
Size: 157 7 102 138 841
Fragments arranged by size:
841 157 138 102 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 82 1245
Size: 82 1163
BtsI GCAGTG_nn'
Cuts at: 0 432 1245
Size: 432 813
ClaI AT'CG_AT
Cuts at: 0 677 1245
Size: 677 568
DraIII CAC_nnn'GTG
Cuts at: 0 471 1245
Size: 471 774
EaeI y'GGCC_r
Cuts at: 0 47 290 377 401 1245
Size: 47 243 87 24 844
Fragments arranged by size:
844 243 87 47 24
EarI CTCTTCn'nnn_
Cuts at: 0 783 920 1245
Size: 783 137 325
Fragments arranged by size:
783 325 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 277 358 1245
Size: 227 50 81 887
Fragments arranged by size:
887 227 81 50
EcoO109I rG'GnC_Cy
Cuts at: 0 249 351 966 1092 1245
Size: 249 102 615 126 153
Fragments arranged by size:
615 249 153 126 102
HaeII r_GCGC'y
Cuts at: 0 345 1023 1245
Size: 345 678 222
Fragments arranged by size:
678 345 222
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1173 1205 1245
Size: 1173 32 40
Fragments arranged by size:
1173 40 32
HincII GTy'rAC
Cuts at: 0 915 1245
Size: 915 330
KpnI G_GTAC'C
Cuts at: 0 958 1245
Size: 958 287
MscI TGG'CCA
Cuts at: 0 292 403 1245
Size: 292 111 842
Fragments arranged by size:
842 292 111
MslI CAynn'nnrTG
Cuts at: 0 61 805 1245
Size: 61 744 440
Fragments arranged by size:
744 440 61
MspA1I CmG'CkG
Cuts at: 0 39 78 357 399 908 1245
Size: 39 39 279 42 509 337
Fragments arranged by size:
509 337 279 42 39 39
NcoI C'CATG_G
Cuts at: 0 266 1245
Size: 266 979
NheI G'CTAG_C
Cuts at: 0 223 564 1245
Size: 223 341 681
Fragments arranged by size:
681 341 223
NspI r_CATG'y
Cuts at: 0 447 804 1072 1245
Size: 447 357 268 173
Fragments arranged by size:
447 357 268 173
PasI CC'CwG_GG
Cuts at: 0 971 1245
Size: 971 274
PfoI T'CCnGG_A
Cuts at: 0 215 1245
Size: 215 1030
PpuMI rG'GwC_Cy
Cuts at: 0 966 1092 1245
Size: 966 126 153
Fragments arranged by size:
966 153 126
PspOMI G'GGCC_C
Cuts at: 0 351 1245
Size: 351 894
PstI C_TGCA'G
Cuts at: 0 77 1245
Size: 77 1168
PvuII CAG'CTG
Cuts at: 0 39 78 399 1245
Size: 39 39 321 846
Fragments arranged by size:
846 321 39 39
RsrII CG'GwC_CG
Cuts at: 0 161 1245
Size: 161 1084
SapI GCTCTTCn'nnn_
Cuts at: 0 783 1245
Size: 783 462
SexAI A'CCwGG_T
Cuts at: 0 170 836 1245
Size: 170 666 409
Fragments arranged by size:
666 409 170
SfcI C'TryA_G
Cuts at: 0 73 243 1245
Size: 73 170 1002
Fragments arranged by size:
1002 170 73
SmaI CCC'GGG
Cuts at: 0 511 585 1245
Size: 511 74 660
Fragments arranged by size:
660 511 74
SphI G_CATG'C
Cuts at: 0 804 1245
Size: 804 441
SspI AAT'ATT
Cuts at: 0 133 1245
Size: 133 1112
StuI AGG'CCT
Cuts at: 0 716 1057 1245
Size: 716 341 188
Fragments arranged by size:
716 341 188
StyI C'CwwG_G
Cuts at: 0 266 383 958 1245
Size: 266 117 575 287
Fragments arranged by size:
575 287 266 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 906 1245
Size: 906 339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 986 1018 1245
Size: 986 32 227
Fragments arranged by size:
986 227 32
Tth111I GACn'n_nGTC
Cuts at: 0 172 1245
Size: 172 1073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 666 1245
Size: 666 579
XmaI C'CCGG_G
Cuts at: 0 509 583 1245
Size: 509 74 662
Fragments arranged by size:
662 509 74
Enzymes that do cut:
AccI Acc65I AcuI AfeI AlwNI ApaI ApaLI AseI
AvaI BanI BanII BbsI BbvCI BglI BlpI Bme1580I
BmtI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
DraIII EaeI EarI Eco57MI EcoO109I HaeII Hin4I HincII
KpnI MscI MslI MspA1I NcoI NheI NspI PasI
PfoI PpuMI PspOMI PstI PvuII RsrII SapI SexAI
SfcI SmaI SphI SspI StuI StyI TaqII TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApoI AscI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BfrBI BglII BmgBI BmrI
BplI BpuEI BsaI BsaBI BsiEI BsiWI BsmI BsmBI
BspHI BsrBI BsrDI BsrFI BsrGI BstEII BstZ17I Bsu36I
CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SwaI TatI XbaI XhoI XmnI
ZraI