Restriction map for
pFN21AE6331
(Linear) (Six-base) MAPSORT of: KIEE6331.seq Check: 4065 from: 1 to: 1197
KIEE6331.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AccI GT'mk_AC
Cuts at: 0 947 1197
Size: 947 250
AflIII A'CryG_T
Cuts at: 0 332 334 1197
Size: 332 2 863
Fragments arranged by size:
863 332 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1041 1197
Size: 1041 156
AlwNI CAG_nnn'CTG
Cuts at: 0 190 1056 1197
Size: 190 866 141
Fragments arranged by size:
866 190 141
AvaI C'yCGr_G
Cuts at: 0 500 1197
Size: 500 697
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1081 1114 1197
Size: 1081 33 83
Fragments arranged by size:
1081 83 33
BanI G'GyrC_C
Cuts at: 0 32 462 726 1197
Size: 32 430 264 471
Fragments arranged by size:
471 430 264 32
BanII G_rGCy'C
Cuts at: 0 398 1082 1197
Size: 398 684 115
Fragments arranged by size:
684 398 115
BbsI GAAGACnn'nnnn_
Cuts at: 0 931 1197
Size: 931 266
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 354 388 1197
Size: 354 34 809
Fragments arranged by size:
809 354 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 297 1197
Size: 297 900
BglI GCCn_nnn'nGGC
Cuts at: 0 650 976 1197
Size: 650 326 221
Fragments arranged by size:
650 326 221
Bme1580I G_kGCm'C
Cuts at: 0 35 467 966 1197
Size: 35 432 499 231
Fragments arranged by size:
499 432 231 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 22 1197
Size: 22 1175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 328 1010 1014 1197
Size: 239 89 682 4 183
Fragments arranged by size:
682 239 183 89 4
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 1197
Size: 694 503
BsaI GGTCTCn'nnnn_
Cuts at: 0 215 811 1197
Size: 215 596 386
Fragments arranged by size:
596 386 215
BsaAI yAC'GTr
Cuts at: 0 208 335 1197
Size: 208 127 862
Fragments arranged by size:
862 208 127
BsaBI GATnn'nnATC
Cuts at: 0 110 1197
Size: 110 1087
BsaWI w'CCGG_w
Cuts at: 0 320 1197
Size: 320 877
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 604 634 1197
Size: 604 30 563
Fragments arranged by size:
604 563 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 500 687 1197
Size: 500 187 510
Fragments arranged by size:
510 500 187
BseYI C'CCAG_C
Cuts at: 0 826 1197
Size: 826 371
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 105 615 1197
Size: 105 510 582
Fragments arranged by size:
582 510 105
BsiHKAI G_wGCw'C
Cuts at: 0 304 398 1197
Size: 304 94 799
Fragments arranged by size:
799 304 94
Bsp1286I G_dGCh'C
Cuts at: 0 35 304 398 467 966 1082 1197
Size: 35 269 94 69 499 116 115
Fragments arranged by size:
499 269 116 115 94 69 35
BstXI CCAn_nnnn'nTGG
Cuts at: 0 798 1005 1120 1197
Size: 798 207 115 77
Fragments arranged by size:
798 207 115 77
BstYI r'GATC_y
Cuts at: 0 5 1197
Size: 5 1192
BtgI C'CryG_G
Cuts at: 0 899 1197
Size: 899 298
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1092 1197
Size: 1092 105
BtsI GCAGTG_nn'
Cuts at: 0 632 1197
Size: 632 565
EaeI y'GGCC_r
Cuts at: 0 54 1007 1172 1197
Size: 54 953 165 25
Fragments arranged by size:
953 165 54 25
EarI CTCTTCn'nnn_
Cuts at: 0 219 595 1197
Size: 219 376 602
Fragments arranged by size:
602 376 219
EcoICRI GAG'CTC
Cuts at: 0 396 1197
Size: 396 801
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 328 1010 1014 1197
Size: 239 89 682 4 183
Fragments arranged by size:
682 239 183 89 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 98 1197
Size: 98 1099
EcoO109I rG'GnC_Cy
Cuts at: 0 91 1197
Size: 91 1106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 212 244 604 636 1197
Size: 212 32 360 32 561
Fragments arranged by size:
561 360 212 32 32
MfeI C'AATT_G
Cuts at: 0 295 1197
Size: 295 902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1197
Size: 901 296
MscI TGG'CCA
Cuts at: 0 1009 1174 1197
Size: 1009 165 23
Fragments arranged by size:
1009 165 23
MslI CAynn'nnrTG
Cuts at: 0 796 1003 1197
Size: 796 207 194
Fragments arranged by size:
796 207 194
MspA1I CmG'CkG
Cuts at: 0 446 1197
Size: 446 751
NcoI C'CATG_G
Cuts at: 0 899 1197
Size: 899 298
NspI r_CATG'y
Cuts at: 0 262 946 970 1197
Size: 262 684 24 227
Fragments arranged by size:
684 262 227 24
PasI CC'CwG_GG
Cuts at: 0 468 1197
Size: 468 729
PflMI CCAn_nnn'nTGG
Cuts at: 0 156 1056 1197
Size: 156 900 141
Fragments arranged by size:
900 156 141
PfoI T'CCnGG_A
Cuts at: 0 65 276 1197
Size: 65 211 921
Fragments arranged by size:
921 211 65
PmlI CAC'GTG
Cuts at: 0 335 1197
Size: 335 862
PpuMI rG'GwC_Cy
Cuts at: 0 91 1197
Size: 91 1106
SacI G_AGCT'C
Cuts at: 0 398 1197
Size: 398 799
SfcI C'TryA_G
Cuts at: 0 764 1197
Size: 764 433
SmlI C'TyrA_G
Cuts at: 0 709 1197
Size: 709 488
StyI C'CwwG_G
Cuts at: 0 94 638 810 899 1197
Size: 94 544 172 89 298
Fragments arranged by size:
544 298 172 94 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 163 1197
Size: 163 1034
Tth111I GACn'n_nGTC
Cuts at: 0 190 1197
Size: 190 1007
Enzymes that do cut:
AccI AflIII AlfI AlwNI AvaI BaeI BanI BanII
BbsI BcgI BciVI BglI Bme1580I BmrI BpmI BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BstXI BstYI BtgI BtgZI BtsI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I MfeI MmeI
MscI MslI MspA1I NcoI NspI PasI PflMI PfoI
PmlI PpuMI SacI SfcI SmlI StyI TaqII Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BbeI BbvCI BclI BfrBI BglII
BlpI BmgBI BmtI BplI Bpu10I BsaHI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI XbaI XcmI
XhoI XmaI XmnI ZraI