Restriction map for
pFN21AE6330
(Linear) (Six-base) MAPSORT of: KIEE6330.seq Check: 4075 from: 1 to: 1170
KIEE6330.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AccI GT'mk_AC
Cuts at: 0 302 1170
Size: 302 868
Acc65I G'GTAC_C
Cuts at: 0 766 1170
Size: 766 404
AgeI A'CCGG_T
Cuts at: 0 113 1170
Size: 113 1057
AlwNI CAG_nnn'CTG
Cuts at: 0 733 1023 1170
Size: 733 290 147
Fragments arranged by size:
733 290 147
ApaI G_GGCC'C
Cuts at: 0 20 87 1059 1170
Size: 20 67 972 111
Fragments arranged by size:
972 111 67 20
AvaI C'yCGr_G
Cuts at: 0 12 1170
Size: 12 1158
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 758 791 1170
Size: 758 33 379
Fragments arranged by size:
758 379 33
BanI G'GyrC_C
Cuts at: 0 201 766 1137 1170
Size: 201 565 371 33
Fragments arranged by size:
565 371 201 33
BanII G_rGCy'C
Cuts at: 0 20 87 901 954 1059 1170
Size: 20 67 814 53 105 111
Fragments arranged by size:
814 111 105 67 53 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 173 638 1170
Size: 173 465 532
Fragments arranged by size:
532 465 173
BciVI GTATCCnnnnn_n'
Cuts at: 0 303 1170
Size: 303 867
Bme1580I G_kGCm'C
Cuts at: 0 20 66 87 206 491 694 1059 1170
Size: 20 46 21 119 285 203 365 111
Fragments arranged by size:
365 285 203 119 111 46 21 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 790 998 1170
Size: 790 208 172
Fragments arranged by size:
790 208 172
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 375 1170
Size: 375 795
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 331 358 440 463 677 755 773
Size: 308 23 27 82 23 214 78 18
Cuts at: 773 853 996 1170
Size: 80 143 174
Fragments arranged by size:
308 214 174 143 82 80 78 27
23 23 18
Bpu10I CC'TnA_GC
Cuts at: 0 514 1170
Size: 514 656
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1128 1170
Size: 1128 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 349 1170
Size: 349 821
BsaWI w'CCGG_w
Cuts at: 0 113 1170
Size: 113 1057
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 125 155 361 391 650 680 728 746
Size: 125 30 206 30 259 30 48 18
Cuts at: 746 758 776 895 925 938 968 1170
Size: 12 18 119 30 13 30 202
Fragments arranged by size:
259 206 202 125 119 48 30 30
30 30 30 18 18 13 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 452 850 1170
Size: 452 398 320
Fragments arranged by size:
452 398 320
BseYI C'CCAG_C
Cuts at: 0 86 188 793 1170
Size: 86 102 605 377
Fragments arranged by size:
605 377 102 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1170
Size: 82 1088
BsiHKAI G_wGCw'C
Cuts at: 0 727 901 1170
Size: 727 174 269
Fragments arranged by size:
727 269 174
BsmBI CGTCTCn'nnnn_
Cuts at: 0 481 1170
Size: 481 689
Bsp1286I G_dGCh'C
Cuts at: 0 20 66 87 206 491 694 727 901
Size: 20 46 21 119 285 203 33 174
Cuts at: 901 954 1059 1170
Size: 53 105 111
Fragments arranged by size:
285 203 174 119 111 105 53 46
33 21 20
BsrDI GCAATG_nn'
Cuts at: 0 866 1170
Size: 866 304
BsrFI r'CCGG_y
Cuts at: 0 113 1170
Size: 113 1057
BssSI C'ACGA_G
Cuts at: 0 947 1170
Size: 947 223
BstAPI GCAn_nnn'nTGC
Cuts at: 0 748 1170
Size: 748 422
BstEII G'GTnAC_C
Cuts at: 0 24 1170
Size: 24 1146
BtgI C'CryG_G
Cuts at: 0 193 615 650 966 1170
Size: 193 422 35 316 204
Fragments arranged by size:
422 316 204 193 35
BtsI GCAGTG_nn'
Cuts at: 0 692 749 1170
Size: 692 57 421
Fragments arranged by size:
692 421 57
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 538 573 1170
Size: 538 35 597
Fragments arranged by size:
597 538 35
EaeI y'GGCC_r
Cuts at: 0 958 1170
Size: 958 212
EcoICRI GAG'CTC
Cuts at: 0 899 1170
Size: 899 271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 331 358 440 463 677 755 773
Size: 308 23 27 82 23 214 78 18
Cuts at: 773 853 996 1170
Size: 80 143 174
Fragments arranged by size:
308 214 174 143 82 80 78 27
23 23 18
EcoO109I rG'GnC_Cy
Cuts at: 0 16 17 730 828 923 1020 1055 1170
Size: 16 1 713 98 95 97 35 115
Fragments arranged by size:
713 115 98 97 95 35 16 1
EcoRV GAT'ATC
Cuts at: 0 852 1170
Size: 852 318
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 163 1170
Size: 163 1007
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 650 682 895 927 1170
Size: 650 32 213 32 243
Fragments arranged by size:
650 243 213 32 32
KpnI G_GTAC'C
Cuts at: 0 770 1170
Size: 770 400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 756 1170
Size: 756 414
MspA1I CmG'CkG
Cuts at: 0 90 374 544 1170
Size: 90 284 170 626
Fragments arranged by size:
626 284 170 90
NcoI C'CATG_G
Cuts at: 0 193 650 966 1170
Size: 193 457 316 204
Fragments arranged by size:
457 316 204 193
PflMI CCAn_nnn'nTGG
Cuts at: 0 967 1170
Size: 967 203
PfoI T'CCnGG_A
Cuts at: 0 316 347 1170
Size: 316 31 823
Fragments arranged by size:
823 316 31
PpuMI rG'GwC_Cy
Cuts at: 0 923 1170
Size: 923 247
PspOMI G'GGCC_C
Cuts at: 0 16 83 1055 1170
Size: 16 67 972 115
Fragments arranged by size:
972 115 67 16
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 428 460 1170
Size: 428 32 710
Fragments arranged by size:
710 428 32
PstI C_TGCA'G
Cuts at: 0 744 1170
Size: 744 426
PvuII CAG'CTG
Cuts at: 0 90 374 1170
Size: 90 284 796
Fragments arranged by size:
796 284 90
SacI G_AGCT'C
Cuts at: 0 901 1170
Size: 901 269
SanDI GG'GwC_CC
Cuts at: 0 923 1170
Size: 923 247
SfcI C'TryA_G
Cuts at: 0 369 740 1170
Size: 369 371 430
Fragments arranged by size:
430 371 369
SmaI CCC'GGG
Cuts at: 0 14 1170
Size: 14 1156
SmlI C'TyrA_G
Cuts at: 0 1107 1170
Size: 1107 63
StuI AGG'CCT
Cuts at: 0 981 1170
Size: 981 189
StyI C'CwwG_G
Cuts at: 0 193 296 529 650 966 1170
Size: 193 103 233 121 316 204
Fragments arranged by size:
316 233 204 193 121 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1108 1170
Size: 1108 62
TatI w'GTAC_w
Cuts at: 0 444 1170
Size: 444 726
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 125 157 728 746 760 778 938 970
Size: 125 32 571 18 14 18 160 32
Cuts at: 970 1170
Size: 200
Fragments arranged by size:
571 200 160 125 32 32 18 18
14
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1153 1170
Size: 1153 17
XmaI C'CCGG_G
Cuts at: 0 12 1170
Size: 12 1158
Enzymes that do cut:
AccI Acc65I AgeI AlwNI ApaI AvaI BaeI BanI
BanII BbsI BciVI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BsrDI BsrFI BssSI BstAPI BstEII BtgI
BtsI CspCI EaeI EcoICRI Eco57MI EcoO109I EcoRV FalI
Hin4I KpnI MmeI MspA1I NcoI PflMI PfoI PpuMI
PspOMI PsrI PstI PvuII SacI SanDI SfcI SmaI
SmlI StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BbeI BbvCI BcgI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmI BspEI BspHI BspMI BsrBI BsrGI BssHII
BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EagI EarI EciI EcoNI EcoRI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI MscI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmnI ZraI