Restriction map for
pFN21AE6326
(Linear) (Six-base) MAPSORT of: KIEE6326.seq Check: 8051 from: 1 to: 1290
KIEE6326.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AclI AA'CG_TT
Cuts at: 0 1074 1290
Size: 1074 216
AflIII A'CryG_T
Cuts at: 0 30 1175 1290
Size: 30 1145 115
Fragments arranged by size:
1145 115 30
AlwNI CAG_nnn'CTG
Cuts at: 0 523 1290
Size: 523 767
ApoI r'AATT_y
Cuts at: 0 143 910 1290
Size: 143 767 380
Fragments arranged by size:
767 380 143
AvaI C'yCGr_G
Cuts at: 0 1031 1290
Size: 1031 259
BciVI GTATCCnnnnn_n'
Cuts at: 0 762 1165 1290
Size: 762 403 125
Fragments arranged by size:
762 403 125
Bme1580I G_kGCm'C
Cuts at: 0 816 1290
Size: 816 474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 266 545 1039 1290
Size: 146 120 279 494 251
Fragments arranged by size:
494 279 251 146 120
BsaWI w'CCGG_w
Cuts at: 0 88 179 1290
Size: 88 91 1111
Fragments arranged by size:
1111 91 88
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 29 1290
Size: 29 1261
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 379 1290
Size: 379 911
Bsp1286I G_dGCh'C
Cuts at: 0 816 1290
Size: 816 474
BspEI T'CCGG_A
Cuts at: 0 88 179 1290
Size: 88 91 1111
Fragments arranged by size:
1111 91 88
BspHI T'CATG_A
Cuts at: 0 660 851 1290
Size: 660 191 439
Fragments arranged by size:
660 439 191
BsrDI GCAATG_nn'
Cuts at: 0 518 1290
Size: 518 772
BsrGI T'GTAC_A
Cuts at: 0 1149 1290
Size: 1149 141
BstBI TT'CG_AA
Cuts at: 0 147 1290
Size: 147 1143
BstYI r'GATC_y
Cuts at: 0 336 1290
Size: 336 954
BtgI C'CryG_G
Cuts at: 0 441 681 1158 1290
Size: 441 240 477 132
Fragments arranged by size:
477 441 240 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 90 1290
Size: 90 1200
BtsI GCAGTG_nn'
Cuts at: 0 1088 1290
Size: 1088 202
EaeI y'GGCC_r
Cuts at: 0 2 42 1290
Size: 2 40 1248
Fragments arranged by size:
1248 40 2
EarI CTCTTCn'nnn_
Cuts at: 0 821 1290
Size: 821 469
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1074 1290
Size: 1074 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 266 545 1039 1290
Size: 146 120 279 494 251
Fragments arranged by size:
494 279 251 146 120
EcoO109I rG'GnC_Cy
Cuts at: 0 23 1290
Size: 23 1267
EcoRI G'AATT_C
Cuts at: 0 143 910 1290
Size: 143 767 380
Fragments arranged by size:
767 380 143
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 310 971 1290
Size: 310 661 319
Fragments arranged by size:
661 319 310
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 207 239 328 360 1262 1290
Size: 207 32 89 32 902 28
Fragments arranged by size:
902 207 89 32 32 28
HindIII A'AGCT_T
Cuts at: 0 371 953 1199 1290
Size: 371 582 246 91
Fragments arranged by size:
582 371 246 91
MfeI C'AATT_G
Cuts at: 0 1190 1290
Size: 1190 100
MluI A'CGCG_T
Cuts at: 0 30 1290
Size: 30 1260
MslI CAynn'nnrTG
Cuts at: 0 320 1020 1022 1290
Size: 320 700 2 268
Fragments arranged by size:
700 320 268 2
MspA1I CmG'CkG
Cuts at: 0 63 1059 1290
Size: 63 996 231
Fragments arranged by size:
996 231 63
NcoI C'CATG_G
Cuts at: 0 441 681 1158 1290
Size: 441 240 477 132
Fragments arranged by size:
477 441 240 132
NspI r_CATG'y
Cuts at: 0 1179 1290
Size: 1179 111
PciI A'CATG_T
Cuts at: 0 1175 1290
Size: 1175 115
PflMI CCAn_nnn'nTGG
Cuts at: 0 1231 1290
Size: 1231 59
PpuMI rG'GwC_Cy
Cuts at: 0 23 1290
Size: 23 1267
PshAI GACnn'nnGTC
Cuts at: 0 1049 1290
Size: 1049 241
PstI C_TGCA'G
Cuts at: 0 489 1290
Size: 489 801
PvuII CAG'CTG
Cuts at: 0 63 1059 1290
Size: 63 996 231
Fragments arranged by size:
996 231 63
SanDI GG'GwC_CC
Cuts at: 0 23 1290
Size: 23 1267
SfcI C'TryA_G
Cuts at: 0 485 979 1290
Size: 485 494 311
Fragments arranged by size:
494 485 311
SspI AAT'ATT
Cuts at: 0 306 1108 1290
Size: 306 802 182
Fragments arranged by size:
802 306 182
StyI C'CwwG_G
Cuts at: 0 441 681 1158 1290
Size: 441 240 477 132
Fragments arranged by size:
477 441 240 132
TatI w'GTAC_w
Cuts at: 0 341 1149 1290
Size: 341 808 141
Fragments arranged by size:
808 341 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 587 619 1290
Size: 31 556 32 671
Fragments arranged by size:
671 556 32 31
XbaI T'CTAG_A
Cuts at: 0 105 1290
Size: 105 1185
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1095 1290
Size: 1095 195
XmnI GAAnn'nnTTC
Cuts at: 0 156 199 215 1290
Size: 156 43 16 1075
Fragments arranged by size:
1075 156 43 16
Enzymes that do cut:
AclI AflIII AlwNI ApoI AvaI BciVI Bme1580I BpmI
BsaWI BsaXI BseRI Bsp1286I BspEI BspHI BsrDI BsrGI
BstBI BstYI BtgI BtgZI BtsI EaeI EarI EciI
Eco57MI EcoO109I EcoRI FalI Hin4I HindIII MfeI MluI
MslI MspA1I NcoI NspI PciI PflMI PpuMI PshAI
PstI PvuII SanDI SfcI SspI StyI TatI TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MmeI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI SwaI TaqII Tth111I XhoI XmaI
ZraI