Restriction map for
pFN21AE6318
(Linear) (Six-base) MAPSORT of: KIEE6318.seq Check: 2659 from: 1 to: 1152
KIEE6318.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 150 1059 1152
Size: 150 909 93
Fragments arranged by size:
909 150 93
AccI GT'mk_AC
Cuts at: 0 287 1152
Size: 287 865
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 742 1152
Size: 688 54 410
Fragments arranged by size:
688 410 54
AflIII A'CryG_T
Cuts at: 0 194 356 374 1152
Size: 194 162 18 778
Fragments arranged by size:
778 194 162 18
AleI CACnn'nnGTG
Cuts at: 0 758 1152
Size: 758 394
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 485 1152
Size: 485 667
AlwNI CAG_nnn'CTG
Cuts at: 0 438 666 717 1152
Size: 438 228 51 435
Fragments arranged by size:
438 435 228 51
ApaI G_GGCC'C
Cuts at: 0 1097 1152
Size: 1097 55
ApaLI G'TGCA_C
Cuts at: 0 241 352 1152
Size: 241 111 800
Fragments arranged by size:
800 241 111
AvaI C'yCGr_G
Cuts at: 0 92 205 474 812 1152
Size: 92 113 269 338 340
Fragments arranged by size:
340 338 269 113 92
BamHI G'GATC_C
Cuts at: 0 88 1152
Size: 88 1064
BanI G'GyrC_C
Cuts at: 0 531 773 988 1152
Size: 531 242 215 164
Fragments arranged by size:
531 242 215 164
BanII G_rGCy'C
Cuts at: 0 71 819 1097 1152
Size: 71 748 278 55
Fragments arranged by size:
748 278 71 55
BbvCI CC'TCA_GC
Cuts at: 0 431 913 1152
Size: 431 482 239
Fragments arranged by size:
482 431 239
BclI T'GATC_A
Cuts at: 0 683 1152
Size: 683 469
BlpI GC'TnA_GC
Cuts at: 0 979 1152
Size: 979 173
Bme1580I G_kGCm'C
Cuts at: 0 61 245 356 536 813 1097 1139 1152
Size: 61 184 111 180 277 284 42 13
Fragments arranged by size:
284 277 184 180 111 61 42 13
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 606 1152
Size: 606 546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 769 844 892 898 943 1152
Size: 694 75 75 48 6 45 209
Fragments arranged by size:
694 209 75 75 48 45 6
Bpu10I CC'TnA_GC
Cuts at: 0 431 505 913 1152
Size: 431 74 408 239
Fragments arranged by size:
431 408 239 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 487 1152
Size: 487 665
BsaAI yAC'GTr
Cuts at: 0 351 359 804 1152
Size: 351 8 445 348
Fragments arranged by size:
445 351 348 8
BsaWI w'CCGG_w
Cuts at: 0 85 1152
Size: 85 1067
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 634 646 664 676 984 1014 1152
Size: 634 12 18 12 308 30 138
Fragments arranged by size:
634 308 138 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 598 745 964 967 1152
Size: 145 453 147 219 3 185
Fragments arranged by size:
453 219 185 147 145 3
BseYI C'CCAG_C
Cuts at: 0 393 570 1152
Size: 393 177 582
Fragments arranged by size:
582 393 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 679 1152
Size: 34 645 473
Fragments arranged by size:
645 473 34
BsiHKAI G_wGCw'C
Cuts at: 0 71 245 356 755 1152
Size: 71 174 111 399 397
Fragments arranged by size:
399 397 174 111 71
Bsp1286I G_dGCh'C
Cuts at: 0 61 71 245 356 536 755 813 819
Size: 61 10 174 111 180 219 58 6
Cuts at: 819 1097 1139 1152
Size: 278 42 13
Fragments arranged by size:
278 219 180 174 111 61 58 42
13 10 6
BspHI T'CATG_A
Cuts at: 0 513 1152
Size: 513 639
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 150 453 638 1059 1152
Size: 150 303 185 421 93
Fragments arranged by size:
421 303 185 150 93
BsrBI CCG'CTC
Cuts at: 0 10 135 957 1152
Size: 10 125 822 195
Fragments arranged by size:
822 195 125 10
BssSI C'ACGA_G
Cuts at: 0 244 1152
Size: 244 908
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1075 1143 1152
Size: 1075 68 9
Fragments arranged by size:
1075 68 9
BstYI r'GATC_y
Cuts at: 0 88 218 1152
Size: 88 130 934
Fragments arranged by size:
934 130 88
Bsu36I CC'TnA_GG
Cuts at: 0 310 622 1152
Size: 310 312 530
Fragments arranged by size:
530 312 310
BtgI C'CryG_G
Cuts at: 0 543 649 1152
Size: 543 106 503
Fragments arranged by size:
543 503 106
BtsI GCAGTG_nn'
Cuts at: 0 667 1152
Size: 667 485
DraIII CAC_nnn'GTG
Cuts at: 0 249 344 1152
Size: 249 95 808
Fragments arranged by size:
808 249 95
EaeI y'GGCC_r
Cuts at: 0 251 368 1152
Size: 251 117 784
Fragments arranged by size:
784 251 117
EarI CTCTTCn'nnn_
Cuts at: 0 568 597 1152
Size: 568 29 555
Fragments arranged by size:
568 555 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 868 952 1152
Size: 868 84 200
Fragments arranged by size:
868 200 84
EcoICRI GAG'CTC
Cuts at: 0 69 1152
Size: 69 1083
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 694 742 769 844 892 898 943
Size: 688 6 48 27 75 48 6 45
Cuts at: 943 1152
Size: 209
Fragments arranged by size:
688 209 75 48 48 45 27 6
6
EcoO109I rG'GnC_Cy
Cuts at: 0 333 405 1093 1094 1152
Size: 333 72 688 1 58
Fragments arranged by size:
688 333 72 58 1
FspI TGC'GCA
Cuts at: 0 904 1152
Size: 904 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 675 707 1152
Size: 675 32 445
Fragments arranged by size:
675 445 32
MscI TGG'CCA
Cuts at: 0 370 1152
Size: 370 782
MslI CAynn'nnrTG
Cuts at: 0 238 758 1152
Size: 238 520 394
Fragments arranged by size:
520 394 238
NspI r_CATG'y
Cuts at: 0 198 378 978 1152
Size: 198 180 600 174
Fragments arranged by size:
600 198 180 174
PasI CC'CwG_GG
Cuts at: 0 324 1132 1152
Size: 324 808 20
Fragments arranged by size:
808 324 20
PciI A'CATG_T
Cuts at: 0 194 374 1152
Size: 194 180 778
Fragments arranged by size:
778 194 180
PflMI CCAn_nnn'nTGG
Cuts at: 0 344 393 1152
Size: 344 49 759
Fragments arranged by size:
759 344 49
PfoI T'CCnGG_A
Cuts at: 0 301 1152
Size: 301 851
PmlI CAC'GTG
Cuts at: 0 351 359 1152
Size: 351 8 793
Fragments arranged by size:
793 351 8
PpuMI rG'GwC_Cy
Cuts at: 0 333 405 1152
Size: 333 72 747
Fragments arranged by size:
747 333 72
PshAI GACnn'nnGTC
Cuts at: 0 152 1152
Size: 152 1000
PspOMI G'GGCC_C
Cuts at: 0 1093 1152
Size: 1093 59
PspXI vC'TCGA_Gb
Cuts at: 0 205 1152
Size: 205 947
PstI C_TGCA'G
Cuts at: 0 422 464 635 1056 1080 1104 1148 1152
Size: 422 42 171 421 24 24 44 4
Fragments arranged by size:
422 421 171 44 42 24 24 4
SacI G_AGCT'C
Cuts at: 0 71 1152
Size: 71 1081
SapI GCTCTTCn'nnn_
Cuts at: 0 568 1152
Size: 568 584
ScaI AGT'ACT
Cuts at: 0 736 1152
Size: 736 416
SfcI C'TryA_G
Cuts at: 0 418 460 631 1052 1076 1100 1144 1152
Size: 418 42 171 421 24 24 44 8
Fragments arranged by size:
421 418 171 44 42 24 24 8
SmaI CCC'GGG
Cuts at: 0 814 1152
Size: 814 338
SmlI C'TyrA_G
Cuts at: 0 205 1152
Size: 205 947
SrfI GCCC'GGGC
Cuts at: 0 814 1152
Size: 814 338
StuI AGG'CCT
Cuts at: 0 589 796 1152
Size: 589 207 356
Fragments arranged by size:
589 356 207
StyI C'CwwG_G
Cuts at: 0 638 848 1152
Size: 638 210 304
Fragments arranged by size:
638 304 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 794 1029 1077 1152
Size: 794 235 48 75
Fragments arranged by size:
794 235 75 48
TatI w'GTAC_w
Cuts at: 0 734 1152
Size: 734 418
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 80 112 634 646 666 678 984 1016
Size: 80 32 522 12 20 12 306 32
Cuts at: 1016 1152
Size: 136
Fragments arranged by size:
522 306 136 80 32 32 20 12
12
XhoI C'TCGA_G
Cuts at: 0 205 1152
Size: 205 947
XmaI C'CCGG_G
Cuts at: 0 812 1152
Size: 812 340
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbvCI BclI BlpI
Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BssSI BstAPI BstYI Bsu36I BtgI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoO109I FspI Hin4I MscI
MslI NspI PasI PciI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PstI SacI SapI ScaI SfcI
SmaI SmlI SrfI StuI StyI TaqII TatI TstI
XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BcgI BciVI BfrBI BglI BglII BmgBI BmrI BmtI
BpuEI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI
BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EcoNI EcoRI
EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PpiI PsiI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI Tth111I XbaI XcmI
XmnI ZraI