Restriction map for
pFN21AE6317
(Linear) (Six-base) MAPSORT of: KIEE6317.seq Check: 2084 from: 1 to: 939
KIEE6317.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AccI GT'mk_AC
Cuts at: 0 602 939
Size: 602 337
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 664 878 939
Size: 35 629 214 61
Fragments arranged by size:
629 214 61 35
AflIII A'CryG_T
Cuts at: 0 890 939
Size: 890 49
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 682 939
Size: 682 257
AlwNI CAG_nnn'CTG
Cuts at: 0 13 131 925 939
Size: 13 118 794 14
Fragments arranged by size:
794 118 14 13
ApaI G_GGCC'C
Cuts at: 0 563 939
Size: 563 376
AvaI C'yCGr_G
Cuts at: 0 556 939
Size: 556 383
BanI G'GyrC_C
Cuts at: 0 403 826 939
Size: 403 423 113
Fragments arranged by size:
423 403 113
BanII G_rGCy'C
Cuts at: 0 563 739 939
Size: 563 176 200
Fragments arranged by size:
563 200 176
BbeI G_GCGC'C
Cuts at: 0 407 939
Size: 407 532
BbsI GAAGACnn'nnnn_
Cuts at: 0 23 369 867 939
Size: 23 346 498 72
Fragments arranged by size:
498 346 72 23
Bme1580I G_kGCm'C
Cuts at: 0 563 719 939
Size: 563 156 220
Fragments arranged by size:
563 220 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 715 939
Size: 555 160 224
Fragments arranged by size:
555 224 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 939
Size: 796 143
BsaI GGTCTCn'nnnn_
Cuts at: 0 903 939
Size: 903 36
BsaHI Gr'CG_yC
Cuts at: 0 404 939
Size: 404 535
BsiEI CG_ry'CG
Cuts at: 0 425 747 939
Size: 425 322 192
Fragments arranged by size:
425 322 192
BsiHKAI G_wGCw'C
Cuts at: 0 191 530 581 939
Size: 191 339 51 358
Fragments arranged by size:
358 339 191 51
BsmI GAATG_Cn'
Cuts at: 0 68 678 939
Size: 68 610 261
Fragments arranged by size:
610 261 68
BsmBI CGTCTCn'nnnn_
Cuts at: 0 684 939
Size: 684 255
Bsp1286I G_dGCh'C
Cuts at: 0 191 530 563 581 719 739 939
Size: 191 339 33 18 138 20 200
Fragments arranged by size:
339 200 191 138 33 20 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 116 245 782 939
Size: 116 129 537 157
Fragments arranged by size:
537 157 129 116
BsrFI r'CCGG_y
Cuts at: 0 448 939
Size: 448 491
BssHII G'CGCG_C
Cuts at: 0 693 939
Size: 693 246
BstAPI GCAn_nnn'nTGC
Cuts at: 0 925 939
Size: 925 14
BtgI C'CryG_G
Cuts at: 0 174 267 395 914 939
Size: 174 93 128 519 25
Fragments arranged by size:
519 174 128 93 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 393 939
Size: 393 546
EaeI y'GGCC_r
Cuts at: 0 195 264 422 939
Size: 195 69 158 517
Fragments arranged by size:
517 195 158 69
EagI C'GGCC_G
Cuts at: 0 422 939
Size: 422 517
EarI CTCTTCn'nnn_
Cuts at: 0 124 200 939
Size: 124 76 739
Fragments arranged by size:
739 124 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 69 939
Size: 69 870
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 555 664 715 878 939
Size: 35 520 109 51 163 61
Fragments arranged by size:
520 163 109 61 51 35
EcoO109I rG'GnC_Cy
Cuts at: 0 10 81 156 510 767 939
Size: 10 71 75 354 257 172
Fragments arranged by size:
354 257 172 75 71 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 669 939
Size: 669 270
HaeII r_GCGC'y
Cuts at: 0 407 939
Size: 407 532
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 101 133 392 424 939
Size: 101 32 259 32 515
Fragments arranged by size:
515 259 101 32 32
HincII GTy'rAC
Cuts at: 0 603 939
Size: 603 336
KasI G'GCGC_C
Cuts at: 0 403 939
Size: 403 536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 939
Size: 687 252
MscI TGG'CCA
Cuts at: 0 266 939
Size: 266 673
MslI CAynn'nnrTG
Cuts at: 0 166 257 939
Size: 166 91 682
Fragments arranged by size:
682 166 91
NaeI GCC'GGC
Cuts at: 0 450 939
Size: 450 489
NarI GG'CG_CC
Cuts at: 0 404 939
Size: 404 535
NcoI C'CATG_G
Cuts at: 0 174 395 939
Size: 174 221 544
Fragments arranged by size:
544 221 174
NgoMIV G'CCGG_C
Cuts at: 0 448 939
Size: 448 491
NspI r_CATG'y
Cuts at: 0 33 340 894 939
Size: 33 307 554 45
Fragments arranged by size:
554 307 45 33
PasI CC'CwG_GG
Cuts at: 0 364 939
Size: 364 575
PciI A'CATG_T
Cuts at: 0 890 939
Size: 890 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 396 939
Size: 396 543
PpuMI rG'GwC_Cy
Cuts at: 0 10 81 510 939
Size: 10 71 429 429
Fragments arranged by size:
429 429 71 10
PshAI GACnn'nnGTC
Cuts at: 0 598 939
Size: 598 341
PsiI TTA'TAA
Cuts at: 0 611 939
Size: 611 328
PspOMI G'GGCC_C
Cuts at: 0 559 939
Size: 559 380
PstI C_TGCA'G
Cuts at: 0 127 939
Size: 127 812
PvuI CG_AT'CG
Cuts at: 0 747 939
Size: 747 192
SalI G'TCGA_C
Cuts at: 0 601 939
Size: 601 338
SapI GCTCTTCn'nnn_
Cuts at: 0 200 939
Size: 200 739
SexAI A'CCwGG_T
Cuts at: 0 92 939
Size: 92 847
SfcI C'TryA_G
Cuts at: 0 123 939
Size: 123 816
SfoI GGC'GCC
Cuts at: 0 405 939
Size: 405 534
SmlI C'TyrA_G
Cuts at: 0 811 939
Size: 811 128
SphI G_CATG'C
Cuts at: 0 33 939
Size: 33 906
StyI C'CwwG_G
Cuts at: 0 152 174 395 939
Size: 152 22 221 544
Fragments arranged by size:
544 221 152 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 939
Size: 165 774
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 259 939
Size: 259 680
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbsI Bme1580I BpmI BpuEI BsaI BsaHI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BssHII
BstAPI BtgI BtgZI EaeI EagI EarI EciI Eco57MI
EcoO109I FalI HaeII Hin4I HincII KasI MmeI MscI
MslI NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PpuMI PshAI PsiI PspOMI PstI PvuI SalI
SapI SexAI SfcI SfoI SmlI SphI StyI TaqII
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiWI BspEI
BspHI BsrBI BsrDI BsrGI BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MspA1I NdeI NheI
NotI NruI NsiI PacI PfoI PmeI PmlI PpiI
PspXI PsrI PvuII RsrII SacI SacII SanDI SbfI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI