Restriction map for
pFN21AE6314
(Linear) (Six-base) MAPSORT of: KIEE6314.seq Check: 8166 from: 1 to: 1047
KIEE6314.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AccI GT'mk_AC
Cuts at: 0 348 1047
Size: 348 699
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 217 511 1047
Size: 134 83 294 536
Fragments arranged by size:
536 294 134 83
ApoI r'AATT_y
Cuts at: 0 279 1006 1047
Size: 279 727 41
Fragments arranged by size:
727 279 41
AvrII C'CTAG_G
Cuts at: 0 423 459 1047
Size: 423 36 588
Fragments arranged by size:
588 423 36
BanI G'GyrC_C
Cuts at: 0 80 883 913 1047
Size: 80 803 30 134
Fragments arranged by size:
803 134 80 30
BanII G_rGCy'C
Cuts at: 0 269 1047
Size: 269 778
BbeI G_GCGC'C
Cuts at: 0 887 1047
Size: 887 160
BbsI GAAGACnn'nnnn_
Cuts at: 0 728 958 1047
Size: 728 230 89
Fragments arranged by size:
728 230 89
BclI T'GATC_A
Cuts at: 0 564 1047
Size: 564 483
BglII A'GATC_T
Cuts at: 0 288 1028 1047
Size: 288 740 19
Fragments arranged by size:
740 288 19
Bme1580I G_kGCm'C
Cuts at: 0 85 276 423 455 1047
Size: 85 191 147 32 592
Fragments arranged by size:
592 191 147 85 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 42 1047
Size: 42 1005
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1047
Size: 817 230
BsaHI Gr'CG_yC
Cuts at: 0 884 1047
Size: 884 163
BsaWI w'CCGG_w
Cuts at: 0 37 1047
Size: 37 1010
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 373 403 1047
Size: 373 30 644
Fragments arranged by size:
644 373 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 28 31 269 1047
Size: 25 3 3 238 778
Fragments arranged by size:
778 238 25 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 781 1047
Size: 694 87 266
Fragments arranged by size:
694 266 87
Bsp1286I G_dGCh'C
Cuts at: 0 85 269 276 423 455 1047
Size: 85 184 7 147 32 592
Fragments arranged by size:
592 184 147 85 32 7
BstBI TT'CG_AA
Cuts at: 0 719 1047
Size: 719 328
BstXI CCAn_nnnn'nTGG
Cuts at: 0 250 841 1047
Size: 250 591 206
Fragments arranged by size:
591 250 206
BstYI r'GATC_y
Cuts at: 0 288 1028 1047
Size: 288 740 19
Fragments arranged by size:
740 288 19
Bsu36I CC'TnA_GG
Cuts at: 0 640 1047
Size: 640 407
BtgI C'CryG_G
Cuts at: 0 243 1047
Size: 243 804
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 444 479 1047
Size: 444 35 568
Fragments arranged by size:
568 444 35
DraIII CAC_nnn'GTG
Cuts at: 0 80 1047
Size: 80 967
EarI CTCTTCn'nnn_
Cuts at: 0 110 401 1047
Size: 110 291 646
Fragments arranged by size:
646 291 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 1047
Size: 22 1025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 217 511 817 1047
Size: 134 83 294 306 230
Fragments arranged by size:
306 294 230 134 83
EcoO109I rG'GnC_Cy
Cuts at: 0 652 1047
Size: 652 395
EcoRI G'AATT_C
Cuts at: 0 1006 1047
Size: 1006 41
HaeII r_GCGC'y
Cuts at: 0 887 1047
Size: 887 160
KasI G'GCGC_C
Cuts at: 0 883 1047
Size: 883 164
MslI CAynn'nnrTG
Cuts at: 0 523 755 944 1047
Size: 523 232 189 103
Fragments arranged by size:
523 232 189 103
NarI GG'CG_CC
Cuts at: 0 884 1047
Size: 884 163
NcoI C'CATG_G
Cuts at: 0 243 1047
Size: 243 804
NdeI CA'TA_TG
Cuts at: 0 945 1047
Size: 945 102
PpuMI rG'GwC_Cy
Cuts at: 0 652 1047
Size: 652 395
SfoI GGC'GCC
Cuts at: 0 885 1047
Size: 885 162
SspI AAT'ATT
Cuts at: 0 213 1047
Size: 213 834
StuI AGG'CCT
Cuts at: 0 687 1047
Size: 687 360
StyI C'CwwG_G
Cuts at: 0 243 423 459 532 599 1047
Size: 243 180 36 73 67 448
Fragments arranged by size:
448 243 180 73 67 36
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 584 1047
Size: 584 463
XmnI GAAnn'nnTTC
Cuts at: 0 161 705 1047
Size: 161 544 342
Fragments arranged by size:
544 342 161
Enzymes that do cut:
AccI AcuI ApoI AvrII BanI BanII BbeI BbsI
BclI BglII Bme1580I BplI BpmI BsaHI BsaWI BsaXI
BseRI BsgI Bsp1286I BstBI BstXI BstYI Bsu36I BtgI
CspCI DraIII EarI EciI Eco57MI EcoO109I EcoRI HaeII
KasI MslI NarI NcoI NdeI PpuMI SfoI SspI
StuI StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI BaeI BamHI BbvCI BcgI BciVI
BfrBI BglI BlpI BmgBI BmrI BmtI Bpu10I BpuEI
BsaI BsaAI BsaBI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstEII BstZ17I BtgZI BtsI ClaI
DraI DrdI EaeI EagI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MspA1I NaeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI ZraI