Restriction map for
pFN21AE6310
(Linear) (Six-base) MAPSORT of: KIEE6310.seq Check: 5000 from: 1 to: 1251
KIEE6310.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AccI GT'mk_AC
Cuts at: 0 509 1251
Size: 509 742
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 1251
Size: 401 850
ApaI G_GGCC'C
Cuts at: 0 404 1251
Size: 404 847
ApoI r'AATT_y
Cuts at: 0 781 1072 1251
Size: 781 291 179
Fragments arranged by size:
781 291 179
BanI G'GyrC_C
Cuts at: 0 360 1251
Size: 360 891
BanII G_rGCy'C
Cuts at: 0 101 404 1251
Size: 101 303 847
Fragments arranged by size:
847 303 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 748 1251
Size: 748 503
BbvCI CC'TCA_GC
Cuts at: 0 87 964 1251
Size: 87 877 287
Fragments arranged by size:
877 287 87
BciVI GTATCCnnnnn_n'
Cuts at: 0 339 1251
Size: 339 912
BclI T'GATC_A
Cuts at: 0 870 1251
Size: 870 381
BglI GCCn_nnn'nGGC
Cuts at: 0 691 1251
Size: 691 560
Bme1580I G_kGCm'C
Cuts at: 0 363 404 851 1251
Size: 363 41 447 400
Fragments arranged by size:
447 400 363 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 366 398 1251
Size: 366 32 853
Fragments arranged by size:
853 366 32
Bpu10I CC'TnA_GC
Cuts at: 0 25 87 710 964 1251
Size: 25 62 623 254 287
Fragments arranged by size:
623 287 254 62 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 305 985 1251
Size: 305 680 266
Fragments arranged by size:
680 305 266
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 291 483 1251
Size: 291 192 768
Fragments arranged by size:
768 291 192
BseYI C'CCAG_C
Cuts at: 0 46 163 895 1251
Size: 46 117 732 356
Fragments arranged by size:
732 356 117 46
BsiHKAI G_wGCw'C
Cuts at: 0 1226 1251
Size: 1226 25
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1113 1251
Size: 1113 138
Bsp1286I G_dGCh'C
Cuts at: 0 101 363 404 851 1226 1251
Size: 101 262 41 447 375 25
Fragments arranged by size:
447 375 262 101 41 25
BspHI T'CATG_A
Cuts at: 0 873 1251
Size: 873 378
BsrDI GCAATG_nn'
Cuts at: 0 527 800 1251
Size: 527 273 451
Fragments arranged by size:
527 451 273
BsrGI T'GTAC_A
Cuts at: 0 548 1251
Size: 548 703
BssSI C'ACGA_G
Cuts at: 0 1219 1251
Size: 1219 32
BstXI CCAn_nnnn'nTGG
Cuts at: 0 186 1251
Size: 186 1065
BstYI r'GATC_y
Cuts at: 0 557 586 1251
Size: 557 29 665
Fragments arranged by size:
665 557 29
EaeI y'GGCC_r
Cuts at: 0 188 485 1251
Size: 188 297 766
Fragments arranged by size:
766 297 188
EarI CTCTTCn'nnn_
Cuts at: 0 988 1116 1251
Size: 988 128 135
Fragments arranged by size:
988 135 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 1251
Size: 401 850
EcoO109I rG'GnC_Cy
Cuts at: 0 1098 1251
Size: 1098 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 313 345 1251
Size: 313 32 906
Fragments arranged by size:
906 313 32
MscI TGG'CCA
Cuts at: 0 487 1251
Size: 487 764
MslI CAynn'nnrTG
Cuts at: 0 286 1251
Size: 286 965
MspA1I CmG'CkG
Cuts at: 0 46 963 1251
Size: 46 917 288
Fragments arranged by size:
917 288 46
PasI CC'CwG_GG
Cuts at: 0 316 1251
Size: 316 935
PspOMI G'GGCC_C
Cuts at: 0 400 1251
Size: 400 851
PvuII CAG'CTG
Cuts at: 0 46 963 1251
Size: 46 917 288
Fragments arranged by size:
917 288 46
SapI GCTCTTCn'nnn_
Cuts at: 0 988 1251
Size: 988 263
ScaI AGT'ACT
Cuts at: 0 122 675 1251
Size: 122 553 576
Fragments arranged by size:
576 553 122
SfcI C'TryA_G
Cuts at: 0 41 563 669 1251
Size: 41 522 106 582
Fragments arranged by size:
582 522 106 41
SpeI A'CTAG_T
Cuts at: 0 52 1251
Size: 52 1199
StyI C'CwwG_G
Cuts at: 0 101 530 1251
Size: 101 429 721
Fragments arranged by size:
721 429 101
TatI w'GTAC_w
Cuts at: 0 120 548 673 1251
Size: 120 428 125 578
Fragments arranged by size:
578 428 125 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 849 881 1251
Size: 849 32 370
Fragments arranged by size:
849 370 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 480 1251
Size: 480 771
Enzymes that do cut:
AccI AcuI ApaI ApoI BanI BanII BbsI BbvCI
BciVI BclI BglI Bme1580I BmrI Bpu10I BsaI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspHI BsrDI BsrGI BssSI
BstXI BstYI EaeI EarI Eco57MI EcoO109I Hin4I MscI
MslI MspA1I PasI PspOMI PvuII SapI ScaI SfcI
SpeI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BfrBI
BglII BlpI BmgBI BmtI BplI BpmI BpuEI BsaAI
BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmI
BspEI BspMI BsrBI BsrFI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XhoI XmaI XmnI ZraI