Restriction map for
pFN21AE6308
(Linear) (Six-base) MAPSORT of: KIEE6308.seq Check: 6702 from: 1 to: 1179
KIEE6308.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 636 1179
Size: 636 543
Acc65I G'GTAC_C
Cuts at: 0 596 1179
Size: 596 583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1179
Size: 928 251
AfeI AGC'GCT
Cuts at: 0 221 1179
Size: 221 958
AgeI A'CCGG_T
Cuts at: 0 154 1014 1179
Size: 154 860 165
Fragments arranged by size:
860 165 154
AleI CACnn'nnGTG
Cuts at: 0 436 646 1179
Size: 436 210 533
Fragments arranged by size:
533 436 210
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 274 306 1179
Size: 274 32 873
Fragments arranged by size:
873 274 32
AlwNI CAG_nnn'CTG
Cuts at: 0 733 1128 1179
Size: 733 395 51
Fragments arranged by size:
733 395 51
ApaLI G'TGCA_C
Cuts at: 0 649 1179
Size: 649 530
AvaI C'yCGr_G
Cuts at: 0 161 1179
Size: 161 1018
BanI G'GyrC_C
Cuts at: 0 340 596 1167 1179
Size: 340 256 571 12
Fragments arranged by size:
571 340 256 12
BanII G_rGCy'C
Cuts at: 0 362 1179
Size: 362 817
BbeI G_GCGC'C
Cuts at: 0 344 1179
Size: 344 835
BbsI GAAGACnn'nnnn_
Cuts at: 0 821 916 1179
Size: 821 95 263
Fragments arranged by size:
821 263 95
BbvCI CC'TCA_GC
Cuts at: 0 216 1179
Size: 216 963
BclI T'GATC_A
Cuts at: 0 567 1179
Size: 567 612
BglI GCCn_nnn'nGGC
Cuts at: 0 91 1179
Size: 91 1088
BglII A'GATC_T
Cuts at: 0 1001 1179
Size: 1001 178
Bme1580I G_kGCm'C
Cuts at: 0 653 1172 1179
Size: 653 519 7
Fragments arranged by size:
653 519 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 553 877 970 1179
Size: 169 384 324 93 209
Fragments arranged by size:
384 324 209 169 93
Bpu10I CC'TnA_GC
Cuts at: 0 216 1179
Size: 216 963
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 861 1179
Size: 790 71 318
Fragments arranged by size:
790 318 71
BsaI GGTCTCn'nnnn_
Cuts at: 0 145 1147 1179
Size: 145 1002 32
Fragments arranged by size:
1002 145 32
BsaAI yAC'GTr
Cuts at: 0 881 1179
Size: 881 298
BsaBI GATnn'nnATC
Cuts at: 0 715 1179
Size: 715 464
BsaHI Gr'CG_yC
Cuts at: 0 341 1179
Size: 341 838
BsaWI w'CCGG_w
Cuts at: 0 154 1014 1179
Size: 154 860 165
Fragments arranged by size:
860 165 154
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 565 595 1179
Size: 565 30 584
Fragments arranged by size:
584 565 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 204 967 1129 1132 1179
Size: 204 763 162 3 47
Fragments arranged by size:
763 204 162 47 3
BseYI C'CCAG_C
Cuts at: 0 486 784 1179
Size: 486 298 395
Fragments arranged by size:
486 395 298
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1039 1179
Size: 340 699 140
Fragments arranged by size:
699 340 140
BsiHKAI G_wGCw'C
Cuts at: 0 653 1179
Size: 653 526
Bsp1286I G_dGCh'C
Cuts at: 0 362 653 1172 1179
Size: 362 291 519 7
Fragments arranged by size:
519 362 291 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 617 636 1136 1179
Size: 617 19 500 43
Fragments arranged by size:
617 500 43 19
BsrDI GCAATG_nn'
Cuts at: 0 284 1179
Size: 284 895
BsrFI r'CCGG_y
Cuts at: 0 154 1014 1025 1081 1179
Size: 154 860 11 56 98
Fragments arranged by size:
860 154 98 56 11
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1128 1179
Size: 1128 51
BstEII G'GTnAC_C
Cuts at: 0 1135 1179
Size: 1135 44
BstXI CCAn_nnnn'nTGG
Cuts at: 0 684 1075 1164 1179
Size: 684 391 89 15
Fragments arranged by size:
684 391 89 15
BstYI r'GATC_y
Cuts at: 0 752 1001 1179
Size: 752 249 178
Fragments arranged by size:
752 249 178
BtgI C'CryG_G
Cuts at: 0 632 1179
Size: 632 547
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 783 1179
Size: 783 396
DraIII CAC_nnn'GTG
Cuts at: 0 63 258 552 1179
Size: 63 195 294 627
Fragments arranged by size:
627 294 195 63
EaeI y'GGCC_r
Cuts at: 0 459 686 914 1179
Size: 459 227 228 265
Fragments arranged by size:
459 265 228 227
EarI CTCTTCn'nnn_
Cuts at: 0 692 1042 1179
Size: 692 350 137
Fragments arranged by size:
692 350 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 573 1179
Size: 573 606
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 553 877 928 970 1179
Size: 169 384 324 51 42 209
Fragments arranged by size:
384 324 209 169 51 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 35 1179
Size: 35 1144
EcoO109I rG'GnC_Cy
Cuts at: 0 144 483 1179
Size: 144 339 696
Fragments arranged by size:
696 339 144
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 138 1179
Size: 138 1041
HaeII r_GCGC'y
Cuts at: 0 223 344 733 785 1059 1179
Size: 223 121 389 52 274 120
Fragments arranged by size:
389 274 223 121 120 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 234 266 565 597 850 882 1179
Size: 234 32 299 32 253 32 297
Fragments arranged by size:
299 297 253 234 32 32 32
KasI G'GCGC_C
Cuts at: 0 340 1179
Size: 340 839
KpnI G_GTAC'C
Cuts at: 0 600 1179
Size: 600 579
MscI TGG'CCA
Cuts at: 0 688 1179
Size: 688 491
MslI CAynn'nnrTG
Cuts at: 0 436 646 682 709 1147 1179
Size: 436 210 36 27 438 32
Fragments arranged by size:
438 436 210 36 32 27
NaeI GCC'GGC
Cuts at: 0 1083 1179
Size: 1083 96
NarI GG'CG_CC
Cuts at: 0 341 1179
Size: 341 838
NgoMIV G'CCGG_C
Cuts at: 0 1081 1179
Size: 1081 98
PasI CC'CwG_GG
Cuts at: 0 362 1179
Size: 362 817
PflMI CCAn_nnn'nTGG
Cuts at: 0 552 733 1179
Size: 552 181 446
Fragments arranged by size:
552 446 181
PfoI T'CCnGG_A
Cuts at: 0 755 1040 1179
Size: 755 285 139
Fragments arranged by size:
755 285 139
PstI C_TGCA'G
Cuts at: 0 112 410 614 1001 1179
Size: 112 298 204 387 178
Fragments arranged by size:
387 298 204 178 112
SapI GCTCTTCn'nnn_
Cuts at: 0 1042 1179
Size: 1042 137
SexAI A'CCwGG_T
Cuts at: 0 254 1179
Size: 254 925
SfcI C'TryA_G
Cuts at: 0 108 138 406 610 997 1179
Size: 108 30 268 204 387 182
Fragments arranged by size:
387 268 204 182 108 30
SfoI GGC'GCC
Cuts at: 0 342 1179
Size: 342 837
SmaI CCC'GGG
Cuts at: 0 163 1179
Size: 163 1016
SmlI C'TyrA_G
Cuts at: 0 805 840 1179
Size: 805 35 339
Fragments arranged by size:
805 339 35
StuI AGG'CCT
Cuts at: 0 854 1179
Size: 854 325
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 826 1179
Size: 826 353
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 432 469 528 1038 1072 1179
Size: 432 37 59 510 34 107
Fragments arranged by size:
510 432 107 59 37 34
XmaI C'CCGG_G
Cuts at: 0 161 1179
Size: 161 1018
Enzymes that do cut:
AarI Acc65I AcuI AfeI AgeI AleI AloI AlwNI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BclI
BglI BglII Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BstAPI BstEII BstXI BstYI
BtgI BtgZI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KasI KpnI MscI MslI
NaeI NarI NgoMIV PasI PflMI PfoI PstI SapI
SexAI SfcI SfoI SmaI SmlI StuI TaqII XcmI
XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AhdI AlfI ApaI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BfrBI BlpI BmgBI BmrI BmtI BplI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DrdI EagI EcoICRI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI MfeI MluI MmeI MspA1I NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StyI SwaI TatI TstI Tth111I XbaI
XhoI XmnI ZraI