Restriction map for
pFN21AE6306
(Linear) (Six-base) MAPSORT of: KIEE6306.seq Check: 9084 from: 1 to: 966
KIEE6306.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AccI GT'mk_AC
Cuts at: 0 947 966
Size: 947 19
AflIII A'CryG_T
Cuts at: 0 494 966
Size: 494 472
AleI CACnn'nnGTG
Cuts at: 0 187 469 481 966
Size: 187 282 12 485
Fragments arranged by size:
485 282 187 12
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 884 916 966
Size: 884 32 50
Fragments arranged by size:
884 50 32
AlwNI CAG_nnn'CTG
Cuts at: 0 201 712 966
Size: 201 511 254
Fragments arranged by size:
511 254 201
BanI G'GyrC_C
Cuts at: 0 181 534 834 966
Size: 181 353 300 132
Fragments arranged by size:
353 300 181 132
BanII G_rGCy'C
Cuts at: 0 599 966
Size: 599 367
BclI T'GATC_A
Cuts at: 0 299 966
Size: 299 667
BglI GCCn_nnn'nGGC
Cuts at: 0 550 966
Size: 550 416
BlpI GC'TnA_GC
Cuts at: 0 808 966
Size: 808 158
Bme1580I G_kGCm'C
Cuts at: 0 176 539 966
Size: 176 363 427
Fragments arranged by size:
427 363 176
BmrI ACTGGGnnnn_n'
Cuts at: 0 141 254 966
Size: 141 113 712
Fragments arranged by size:
712 141 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 966
Size: 304 662
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 955 966
Size: 769 186 11
Fragments arranged by size:
769 186 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 884 914 966
Size: 884 30 52
Fragments arranged by size:
884 52 30
BseYI C'CCAG_C
Cuts at: 0 530 966
Size: 530 436
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 966
Size: 769 197
BsiHKAI G_wGCw'C
Cuts at: 0 599 966
Size: 599 367
BsmI GAATG_Cn'
Cuts at: 0 411 966
Size: 411 555
Bsp1286I G_dGCh'C
Cuts at: 0 176 539 599 966
Size: 176 363 60 367
Fragments arranged by size:
367 363 176 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 789 966
Size: 789 177
BsrBI CCG'CTC
Cuts at: 0 120 812 966
Size: 120 692 154
Fragments arranged by size:
692 154 120
BsrDI GCAATG_nn'
Cuts at: 0 793 966
Size: 793 173
BsrFI r'CCGG_y
Cuts at: 0 339 966
Size: 339 627
BstAPI GCAn_nnn'nTGC
Cuts at: 0 765 804 966
Size: 765 39 162
Fragments arranged by size:
765 162 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 144 289 471 483 966
Size: 144 145 182 12 483
Fragments arranged by size:
483 182 145 144 12
BtgI C'CryG_G
Cuts at: 0 476 966
Size: 476 490
BtsI GCAGTG_nn'
Cuts at: 0 364 464 966
Size: 364 100 502
Fragments arranged by size:
502 364 100
ClaI AT'CG_AT
Cuts at: 0 29 966
Size: 29 937
EaeI y'GGCC_r
Cuts at: 0 473 662 966
Size: 473 189 304
Fragments arranged by size:
473 304 189
EarI CTCTTCn'nnn_
Cuts at: 0 148 966
Size: 148 818
EcoICRI GAG'CTC
Cuts at: 0 597 966
Size: 597 369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 966
Size: 304 662
EcoO109I rG'GnC_Cy
Cuts at: 0 780 966
Size: 780 186
HaeII r_GCGC'y
Cuts at: 0 514 966
Size: 514 452
HincII GTy'rAC
Cuts at: 0 390 500 966
Size: 390 110 466
Fragments arranged by size:
466 390 110
MscI TGG'CCA
Cuts at: 0 475 966
Size: 475 491
MslI CAynn'nnrTG
Cuts at: 0 187 469 481 493 829 864 966
Size: 187 282 12 12 336 35 102
Fragments arranged by size:
336 282 187 102 35 12 12
MspA1I CmG'CkG
Cuts at: 0 198 530 547 966
Size: 198 332 17 419
Fragments arranged by size:
419 332 198 17
NaeI GCC'GGC
Cuts at: 0 341 966
Size: 341 625
NgoMIV G'CCGG_C
Cuts at: 0 339 966
Size: 339 627
NspI r_CATG'y
Cuts at: 0 276 498 966
Size: 276 222 468
Fragments arranged by size:
468 276 222
PciI A'CATG_T
Cuts at: 0 494 966
Size: 494 472
PfoI T'CCnGG_A
Cuts at: 0 649 966
Size: 649 317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 884 916 966
Size: 884 32 50
Fragments arranged by size:
884 50 32
PpuMI rG'GwC_Cy
Cuts at: 0 780 966
Size: 780 186
PvuII CAG'CTG
Cuts at: 0 198 966
Size: 198 768
SacI G_AGCT'C
Cuts at: 0 599 966
Size: 599 367
SapI GCTCTTCn'nnn_
Cuts at: 0 148 966
Size: 148 818
SfcI C'TryA_G
Cuts at: 0 327 966
Size: 327 639
SmlI C'TyrA_G
Cuts at: 0 748 934 966
Size: 748 186 32
Fragments arranged by size:
748 186 32
StyI C'CwwG_G
Cuts at: 0 137 518 966
Size: 137 381 448
Fragments arranged by size:
448 381 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 199 351 522 691 966
Size: 199 152 171 169 275
Fragments arranged by size:
275 199 171 169 152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 599 631 966
Size: 599 32 335
Fragments arranged by size:
599 335 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 241 966
Size: 144 97 725
Fragments arranged by size:
725 144 97
XmnI GAAnn'nnTTC
Cuts at: 0 76 966
Size: 76 890
Enzymes that do cut:
AccI AflIII AleI AloI AlwNI BanI BanII BclI
BglI BlpI Bme1580I BmrI BpmI BpuEI BsaXI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstAPI BstXI BtgI BtsI ClaI EaeI EarI EcoICRI
Eco57MI EcoO109I HaeII HincII MscI MslI MspA1I NaeI
NgoMIV NspI PciI PfoI PpiI PpuMI PvuII SacI
SapI SfcI SmlI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BfrBI BglII BmgBI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmBI
BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XhoI XmaI ZraI