Restriction map for
pFN21AE6305
(Linear) (Six-base) MAPSORT of: KIEE6305.seq Check: 677 from: 1 to: 1230
KIEE6305.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AccI GT'mk_AC
Cuts at: 0 1093 1230
Size: 1093 137
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 980 1230
Size: 161 819 250
Fragments arranged by size:
819 250 161
AflIII A'CryG_T
Cuts at: 0 546 1230
Size: 546 684
AleI CACnn'nnGTG
Cuts at: 0 961 1230
Size: 961 269
AlwNI CAG_nnn'CTG
Cuts at: 0 51 241 283 585 1003 1230
Size: 51 190 42 302 418 227
Fragments arranged by size:
418 302 227 190 51 42
ApoI r'AATT_y
Cuts at: 0 358 385 421 1037 1230
Size: 358 27 36 616 193
Fragments arranged by size:
616 358 193 36 27
AvaI C'yCGr_G
Cuts at: 0 647 1230
Size: 647 583
AvrII C'CTAG_G
Cuts at: 0 67 1230
Size: 67 1163
BamHI G'GATC_C
Cuts at: 0 899 1230
Size: 899 331
BanI G'GyrC_C
Cuts at: 0 104 1230
Size: 104 1126
BbeI G_GCGC'C
Cuts at: 0 108 1230
Size: 108 1122
BclI T'GATC_A
Cuts at: 0 972 1230
Size: 972 258
BglI GCCn_nnn'nGGC
Cuts at: 0 476 1230
Size: 476 754
BlpI GC'TnA_GC
Cuts at: 0 194 526 1230
Size: 194 332 704
Fragments arranged by size:
704 332 194
BmgBI CAC'GTC
Cuts at: 0 547 1230
Size: 547 683
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 1230
Size: 263 967
BsaHI Gr'CG_yC
Cuts at: 0 105 1230
Size: 105 1125
BsaWI w'CCGG_w
Cuts at: 0 977 1230
Size: 977 253
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 719 749 1230
Size: 719 30 481
Fragments arranged by size:
719 481 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1133 1230
Size: 1133 97
BseYI C'CCAG_C
Cuts at: 0 856 1230
Size: 856 374
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 1230
Size: 985 245
BsiHKAI G_wGCw'C
Cuts at: 0 87 1019 1194 1230
Size: 87 932 175 36
Fragments arranged by size:
932 175 87 36
BsmBI CGTCTCn'nnnn_
Cuts at: 0 774 1230
Size: 774 456
Bsp1286I G_dGCh'C
Cuts at: 0 87 1019 1194 1230
Size: 87 932 175 36
Fragments arranged by size:
932 175 87 36
BspHI T'CATG_A
Cuts at: 0 402 1230
Size: 402 828
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 793 1230
Size: 793 437
BsrBI CCG'CTC
Cuts at: 0 57 198 1230
Size: 57 141 1032
Fragments arranged by size:
1032 141 57
BsrGI T'GTAC_A
Cuts at: 0 485 1230
Size: 485 745
BstAPI GCAn_nnn'nTGC
Cuts at: 0 9 51 750 1230
Size: 9 42 699 480
Fragments arranged by size:
699 480 42 9
BstYI r'GATC_y
Cuts at: 0 899 1230
Size: 899 331
BstZ17I GTA'TAC
Cuts at: 0 1094 1230
Size: 1094 136
Bsu36I CC'TnA_GG
Cuts at: 0 934 1230
Size: 934 296
BtgI C'CryG_G
Cuts at: 0 531 959 1230
Size: 531 428 271
Fragments arranged by size:
531 428 271
BtsI GCAGTG_nn'
Cuts at: 0 234 727 1001 1076 1230
Size: 234 493 274 75 154
Fragments arranged by size:
493 274 234 154 75
DraI TTT'AAA
Cuts at: 0 390 1230
Size: 390 840
DraIII CAC_nnn'GTG
Cuts at: 0 91 1230
Size: 91 1139
DrdI GACnn_nn'nnGTC
Cuts at: 0 723 1230
Size: 723 507
EaeI y'GGCC_r
Cuts at: 0 1148 1230
Size: 1148 82
EarI CTCTTCn'nnn_
Cuts at: 0 1212 1230
Size: 1212 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 8 1230
Size: 8 1222
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 263 980 1230
Size: 161 102 717 250
Fragments arranged by size:
717 250 161 102
EcoO109I rG'GnC_Cy
Cuts at: 0 29 909 1230
Size: 29 880 321
Fragments arranged by size:
880 321 29
EcoRI G'AATT_C
Cuts at: 0 358 421 1037 1230
Size: 358 63 616 193
Fragments arranged by size:
616 358 193 63
HaeII r_GCGC'y
Cuts at: 0 79 108 1230
Size: 79 29 1122
Fragments arranged by size:
1122 79 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 33 65 1230
Size: 33 32 1165
Fragments arranged by size:
1165 33 32
HincII GTy'rAC
Cuts at: 0 275 1230
Size: 275 955
KasI G'GCGC_C
Cuts at: 0 104 1230
Size: 104 1126
MslI CAynn'nnrTG
Cuts at: 0 961 1012 1230
Size: 961 51 218
Fragments arranged by size:
961 218 51
NarI GG'CG_CC
Cuts at: 0 105 1230
Size: 105 1125
NcoI C'CATG_G
Cuts at: 0 959 1230
Size: 959 271
PflMI CCAn_nnn'nTGG
Cuts at: 0 713 1230
Size: 713 517
PfoI T'CCnGG_A
Cuts at: 0 895 902 1230
Size: 895 7 328
Fragments arranged by size:
895 328 7
PpuMI rG'GwC_Cy
Cuts at: 0 909 1230
Size: 909 321
PsiI TTA'TAA
Cuts at: 0 869 1230
Size: 869 361
PstI C_TGCA'G
Cuts at: 0 229 1230
Size: 229 1001
ScaI AGT'ACT
Cuts at: 0 448 1230
Size: 448 782
SfcI C'TryA_G
Cuts at: 0 225 772 1230
Size: 225 547 458
Fragments arranged by size:
547 458 225
SfoI GGC'GCC
Cuts at: 0 106 1230
Size: 106 1124
StuI AGG'CCT
Cuts at: 0 479 1230
Size: 479 751
StyI C'CwwG_G
Cuts at: 0 67 959 1172 1230
Size: 67 892 213 58
Fragments arranged by size:
892 213 67 58
TatI w'GTAC_w
Cuts at: 0 446 485 1230
Size: 446 39 745
Fragments arranged by size:
745 446 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 719 751 1230
Size: 719 32 479
Fragments arranged by size:
719 479 32
Tth111I GACn'n_nGTC
Cuts at: 0 279 1230
Size: 279 951
XmnI GAAnn'nnTTC
Cuts at: 0 993 1230
Size: 993 237
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApoI AvaI AvrII
BamHI BanI BbeI BclI BglI BlpI BmgBI BpmI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrGI BstAPI BstYI BstZ17I
Bsu36I BtgI BtsI DraI DraIII DrdI EaeI EarI
EciI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII KasI
MslI NarI NcoI PflMI PfoI PpuMI PsiI PstI
ScaI SfcI SfoI StuI StyI TatI TstI Tth111I
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI BaeI
BanII BbsI BbvCI BcgI BciVI BfrBI BglII Bme1580I
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsiEI BsiWI BsmI BspEI BsrDI BsrFI BssHII BssSI
BstBI BstEII BstXI BtgZI ClaI CspCI EagI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI MscI MspA1I NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TaqII XbaI XcmI
XhoI XmaI ZraI