Restriction map for
pFN21AE6303
(Linear) (Six-base) MAPSORT of: KIEE6303.seq Check: 842 from: 1 to: 1041
KIEE6303.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
Acc65I G'GTAC_C
Cuts at: 0 53 598 1041
Size: 53 545 443
Fragments arranged by size:
545 443 53
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 366 960 1041
Size: 262 104 594 81
Fragments arranged by size:
594 262 104 81
ApaI G_GGCC'C
Cuts at: 0 9 1041
Size: 9 1032
ApoI r'AATT_y
Cuts at: 0 214 223 1041
Size: 214 9 818
Fragments arranged by size:
818 214 9
AvaI C'yCGr_G
Cuts at: 0 8 112 1041
Size: 8 104 929
Fragments arranged by size:
929 104 8
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 582 615 1041
Size: 582 33 426
Fragments arranged by size:
582 426 33
BanI G'GyrC_C
Cuts at: 0 53 234 598 1041
Size: 53 181 364 443
Fragments arranged by size:
443 364 181 53
BanII G_rGCy'C
Cuts at: 0 9 1041
Size: 9 1032
BbsI GAAGACnn'nnnn_
Cuts at: 0 960 1041
Size: 960 81
BbvCI CC'TCA_GC
Cuts at: 0 284 1041
Size: 284 757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 93 127 890 924 1041
Size: 93 34 763 34 117
Fragments arranged by size:
763 117 93 34 34
BfrBI ATG'CAT
Cuts at: 0 456 1041
Size: 456 585
BglI GCCn_nnn'nGGC
Cuts at: 0 288 1041
Size: 288 753
BlpI GC'TnA_GC
Cuts at: 0 524 1041
Size: 524 517
Bme1580I G_kGCm'C
Cuts at: 0 9 1041
Size: 9 1032
Bpu10I CC'TnA_GC
Cuts at: 0 284 1041
Size: 284 757
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 1041
Size: 324 717
BsmI GAATG_Cn'
Cuts at: 0 867 1041
Size: 867 174
Bsp1286I G_dGCh'C
Cuts at: 0 9 1041
Size: 9 1032
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 65 1041
Size: 65 976
BsrDI GCAATG_nn'
Cuts at: 0 602 1041
Size: 602 439
BstBI TT'CG_AA
Cuts at: 0 910 1041
Size: 910 131
BstXI CCAn_nnnn'nTGG
Cuts at: 0 511 609 754 882 1041
Size: 511 98 145 128 159
Fragments arranged by size:
511 159 145 128 98
BtgI C'CryG_G
Cuts at: 0 695 753 875 1011 1041
Size: 695 58 122 136 30
Fragments arranged by size:
695 136 122 58 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 932 1041
Size: 932 109
BtsI GCAGTG_nn'
Cuts at: 0 269 298 1041
Size: 269 29 743
Fragments arranged by size:
743 269 29
EaeI y'GGCC_r
Cuts at: 0 732 1041
Size: 732 309
EarI CTCTTCn'nnn_
Cuts at: 0 281 382 1041
Size: 281 101 659
Fragments arranged by size:
659 281 101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 366 960 1041
Size: 262 104 594 81
Fragments arranged by size:
594 262 104 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 467 1041
Size: 467 574
EcoO109I rG'GnC_Cy
Cuts at: 0 5 1041
Size: 5 1036
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 396 1041
Size: 396 645
HaeII r_GCGC'y
Cuts at: 0 17 351 1041
Size: 17 334 690
Fragments arranged by size:
690 334 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 176 208 1041
Size: 176 32 833
Fragments arranged by size:
833 176 32
KpnI G_GTAC'C
Cuts at: 0 57 602 1041
Size: 57 545 439
Fragments arranged by size:
545 439 57
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 903 1041
Size: 612 291 138
Fragments arranged by size:
612 291 138
MscI TGG'CCA
Cuts at: 0 734 1041
Size: 734 307
MslI CAynn'nnrTG
Cuts at: 0 752 880 1041
Size: 752 128 161
Fragments arranged by size:
752 161 128
MspA1I CmG'CkG
Cuts at: 0 165 288 523 1041
Size: 165 123 235 518
Fragments arranged by size:
518 235 165 123
NcoI C'CATG_G
Cuts at: 0 695 875 1041
Size: 695 180 166
Fragments arranged by size:
695 180 166
NdeI CA'TA_TG
Cuts at: 0 765 1041
Size: 765 276
NsiI A_TGCA'T
Cuts at: 0 458 1041
Size: 458 583
PacI TTA_AT'TAA
Cuts at: 0 1037 1041
Size: 1037 4
PflMI CCAn_nnn'nTGG
Cuts at: 0 787 1041
Size: 787 254
PsiI TTA'TAA
Cuts at: 0 229 1041
Size: 229 812
PspOMI G'GGCC_C
Cuts at: 0 5 1041
Size: 5 1036
PvuII CAG'CTG
Cuts at: 0 165 523 1041
Size: 165 358 518
Fragments arranged by size:
518 358 165
SapI GCTCTTCn'nnn_
Cuts at: 0 281 382 1041
Size: 281 101 659
Fragments arranged by size:
659 281 101
SmaI CCC'GGG
Cuts at: 0 10 1041
Size: 10 1031
SspI AAT'ATT
Cuts at: 0 995 1041
Size: 995 46
StyI C'CwwG_G
Cuts at: 0 695 875 1041
Size: 695 180 166
Fragments arranged by size:
695 180 166
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 600 1041
Size: 600 441
XmaI C'CCGG_G
Cuts at: 0 8 1041
Size: 8 1033
XmnI GAAnn'nnTTC
Cuts at: 0 249 1041
Size: 249 792
Enzymes that do cut:
Acc65I AcuI ApaI ApoI AvaI BaeI BanI BanII
BbsI BbvCI BcgI BfrBI BglI BlpI Bme1580I Bpu10I
BsgI BsmI Bsp1286I BspMI BsrDI BstBI BstXI BtgI
BtgZI BtsI EaeI EarI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I KpnI MmeI MscI MslI MspA1I NcoI
NdeI NsiI PacI PflMI PsiI PspOMI PvuII SapI
SmaI SspI StyI TaqII XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BamHI BbeI BciVI BclI BglII BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI NspI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TatI
TstI Tth111I XbaI XcmI XhoI ZraI