Restriction map for
pFN21AE6296
(Linear) (Six-base) MAPSORT of: KIEE6296.seq Check: 9316 from: 1 to: 549
KIEE6296.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 12 549
Size: 12 537
AlwNI CAG_nnn'CTG
Cuts at: 0 207 225 342 549
Size: 207 18 117 207
Fragments arranged by size:
207 207 117 18
BanII G_rGCy'C
Cuts at: 0 86 293 549
Size: 86 207 256
Fragments arranged by size:
256 207 86
BglI GCCn_nnn'nGGC
Cuts at: 0 496 549
Size: 496 53
BmgBI CAC'GTC
Cuts at: 0 138 549
Size: 138 411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 166 340 421 549
Size: 100 66 174 81 128
Fragments arranged by size:
174 128 100 81 66
BsaAI yAC'GTr
Cuts at: 0 456 549
Size: 456 93
BsaHI Gr'CG_yC
Cuts at: 0 53 549
Size: 53 496
BseYI C'CCAG_C
Cuts at: 0 11 278 383 444 549
Size: 11 267 105 61 105
Fragments arranged by size:
267 105 105 61 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 549
Size: 400 149
BsiEI CG_ry'CG
Cuts at: 0 534 549
Size: 534 15
BsiHKAI G_wGCw'C
Cuts at: 0 86 549
Size: 86 463
Bsp1286I G_dGCh'C
Cuts at: 0 86 293 549
Size: 86 207 256
Fragments arranged by size:
256 207 86
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 12 233 422 473 549
Size: 12 221 189 51 76
Fragments arranged by size:
221 189 76 51 12
BsrBI CCG'CTC
Cuts at: 0 287 549
Size: 287 262
BsrFI r'CCGG_y
Cuts at: 0 490 549
Size: 490 59
BssSI C'ACGA_G
Cuts at: 0 292 418 549
Size: 292 126 131
Fragments arranged by size:
292 131 126
BstAPI GCAn_nnn'nTGC
Cuts at: 0 225 549
Size: 225 324
BstYI r'GATC_y
Cuts at: 0 348 549
Size: 348 201
BtgI C'CryG_G
Cuts at: 0 299 365 549
Size: 299 66 184
Fragments arranged by size:
299 184 66
EaeI y'GGCC_r
Cuts at: 0 2 234 296 362 531 549
Size: 2 232 62 66 169 18
Fragments arranged by size:
232 169 66 62 18 2
EagI C'GGCC_G
Cuts at: 0 531 549
Size: 531 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 297 549
Size: 297 252
EcoICRI GAG'CTC
Cuts at: 0 84 549
Size: 84 465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 166 340 421 549
Size: 100 66 174 81 128
Fragments arranged by size:
174 128 100 81 66
EcoRV GAT'ATC
Cuts at: 0 255 549
Size: 255 294
FspI TGC'GCA
Cuts at: 0 469 549
Size: 469 80
HincII GTy'rAC
Cuts at: 0 333 549
Size: 333 216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 549
Size: 158 391
MscI TGG'CCA
Cuts at: 0 4 236 298 549
Size: 4 232 62 251
Fragments arranged by size:
251 232 62 4
MspA1I CmG'CkG
Cuts at: 0 360 387 549
Size: 360 27 162
Fragments arranged by size:
360 162 27
NaeI GCC'GGC
Cuts at: 0 492 549
Size: 492 57
NcoI C'CATG_G
Cuts at: 0 299 549
Size: 299 250
NgoMIV G'CCGG_C
Cuts at: 0 490 549
Size: 490 59
NotI GC'GGCC_GC
Cuts at: 0 531 549
Size: 531 18
NspI r_CATG'y
Cuts at: 0 199 549
Size: 199 350
PasI CC'CwG_GG
Cuts at: 0 525 549
Size: 525 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 399 549
Size: 399 150
PfoI T'CCnGG_A
Cuts at: 0 398 549
Size: 398 151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 549
Size: 493 32 24
Fragments arranged by size:
493 32 24
PstI C_TGCA'G
Cuts at: 0 221 549
Size: 221 328
PvuII CAG'CTG
Cuts at: 0 360 387 549
Size: 360 27 162
Fragments arranged by size:
360 162 27
SacI G_AGCT'C
Cuts at: 0 86 549
Size: 86 463
SfcI C'TryA_G
Cuts at: 0 217 549
Size: 217 332
SphI G_CATG'C
Cuts at: 0 199 549
Size: 199 350
StyI C'CwwG_G
Cuts at: 0 273 299 549
Size: 273 26 250
Fragments arranged by size:
273 250 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 436 549
Size: 436 113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 549
Size: 441 108
XmnI GAAnn'nnTTC
Cuts at: 0 154 549
Size: 154 395
Enzymes that do cut:
AarI AlwNI BanII BglI BmgBI BpmI BsaAI BsaHI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BssSI BstAPI BstYI BtgI EaeI EagI EciI EcoICRI
Eco57MI EcoRV FspI HincII MmeI MscI MspA1I NaeI
NcoI NgoMIV NotI NspI PasI PflMI PfoI PpiI
PstI PvuII SacI SfcI SphI StyI TaqII XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EarI EcoNI EcoO109I EcoRI FalI FseI FspAI HaeII
Hin4I HindIII HpaI KasI KpnI MfeI MluI MslI
NarI NdeI NheI NruI NsiI PacI PciI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XhoI XmaI ZraI