Restriction map for
pFN21AE6294
(Linear) (Six-base) MAPSORT of: KIEE6294.seq Check: 2001 from: 1 to: 648
KIEE6294.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 648
Size: 552 96
AgeI A'CCGG_T
Cuts at: 0 274 648
Size: 274 374
AlwNI CAG_nnn'CTG
Cuts at: 0 189 648
Size: 189 459
ApaLI G'TGCA_C
Cuts at: 0 73 648
Size: 73 575
AvaI C'yCGr_G
Cuts at: 0 512 648
Size: 512 136
BanII G_rGCy'C
Cuts at: 0 102 196 621 648
Size: 102 94 425 27
Fragments arranged by size:
425 102 94 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 558 583 648
Size: 558 25 65
Fragments arranged by size:
558 65 25
BclI T'GATC_A
Cuts at: 0 318 648
Size: 318 330
Bme1580I G_kGCm'C
Cuts at: 0 77 152 648
Size: 77 75 496
Fragments arranged by size:
496 77 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 174 358 648
Size: 116 58 184 290
Fragments arranged by size:
290 184 116 58
BsaAI yAC'GTr
Cuts at: 0 78 648
Size: 78 570
BsaBI GATnn'nnATC
Cuts at: 0 448 648
Size: 448 200
BsaWI w'CCGG_w
Cuts at: 0 165 274 648
Size: 165 109 374
Fragments arranged by size:
374 165 109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 62 92 648
Size: 62 30 556
Fragments arranged by size:
556 62 30
BseYI C'CCAG_C
Cuts at: 0 138 177 414 648
Size: 138 39 237 234
Fragments arranged by size:
237 234 138 39
BsiHKAI G_wGCw'C
Cuts at: 0 77 648
Size: 77 571
Bsp1286I G_dGCh'C
Cuts at: 0 77 102 152 196 621 648
Size: 77 25 50 44 425 27
Fragments arranged by size:
425 77 50 44 27 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 527 648
Size: 527 121
BsrDI GCAATG_nn'
Cuts at: 0 542 648
Size: 542 106
BsrFI r'CCGG_y
Cuts at: 0 274 648
Size: 274 374
BssSI C'ACGA_G
Cuts at: 0 454 648
Size: 454 194
BstEII G'GTnAC_C
Cuts at: 0 270 648
Size: 270 378
BtgI C'CryG_G
Cuts at: 0 65 611 648
Size: 65 546 37
Fragments arranged by size:
546 65 37
BtsI GCAGTG_nn'
Cuts at: 0 418 648
Size: 418 230
EaeI y'GGCC_r
Cuts at: 0 236 648
Size: 236 412
EarI CTCTTCn'nnn_
Cuts at: 0 232 391 648
Size: 232 159 257
Fragments arranged by size:
257 232 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 174 358 552 648
Size: 116 58 184 194 96
Fragments arranged by size:
194 184 116 96 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 349 648
Size: 349 299
EcoO109I rG'GnC_Cy
Cuts at: 0 516 648
Size: 516 132
HaeII r_GCGC'y
Cuts at: 0 439 648
Size: 439 209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 485 517 648
Size: 485 32 131
Fragments arranged by size:
485 131 32
HincII GTy'rAC
Cuts at: 0 39 648
Size: 39 609
MslI CAynn'nnrTG
Cuts at: 0 70 454 469 648
Size: 70 384 15 179
Fragments arranged by size:
384 179 70 15
MspA1I CmG'CkG
Cuts at: 0 115 264 292 378 648
Size: 115 149 28 86 270
Fragments arranged by size:
270 149 115 86 28
NcoI C'CATG_G
Cuts at: 0 65 648
Size: 65 583
PmlI CAC'GTG
Cuts at: 0 78 648
Size: 78 570
PpuMI rG'GwC_Cy
Cuts at: 0 516 648
Size: 516 132
PvuII CAG'CTG
Cuts at: 0 115 264 292 378 648
Size: 115 149 28 86 270
Fragments arranged by size:
270 149 115 86 28
SexAI A'CCwGG_T
Cuts at: 0 585 648
Size: 585 63
SfcI C'TryA_G
Cuts at: 0 219 287 648
Size: 219 68 361
Fragments arranged by size:
361 219 68
SmaI CCC'GGG
Cuts at: 0 514 648
Size: 514 134
StyI C'CwwG_G
Cuts at: 0 30 65 477 648
Size: 30 35 412 171
Fragments arranged by size:
412 171 35 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 62 94 297 329 648
Size: 62 32 203 32 319
Fragments arranged by size:
319 203 62 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 253 648
Size: 253 395
XmaI C'CCGG_G
Cuts at: 0 512 648
Size: 512 136
Enzymes that do cut:
AcuI AgeI AlwNI ApaLI AvaI BanII BbsI BclI
Bme1580I BpmI BsaAI BsaBI BsaWI BsaXI BseYI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BssSI BstEII BtgI BtsI
EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
MslI MspA1I NcoI PmlI PpuMI PvuII SexAI SfcI
SmaI StyI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaI BsaHI BseRI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XhoI XmnI ZraI