Restriction map for
pFN21AE6292
(Linear) (Six-base) MAPSORT of: KIEE6292.seq Check: 4344 from: 1 to: 903
KIEE6292.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
Acc65I G'GTAC_C
Cuts at: 0 751 903
Size: 751 152
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 903
Size: 175 728
AflII C'TTAA_G
Cuts at: 0 759 903
Size: 759 144
ApoI r'AATT_y
Cuts at: 0 659 707 903
Size: 659 48 196
Fragments arranged by size:
659 196 48
AvaI C'yCGr_G
Cuts at: 0 519 903
Size: 519 384
BanI G'GyrC_C
Cuts at: 0 196 751 903
Size: 196 555 152
Fragments arranged by size:
555 196 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 163 903
Size: 163 740
BciVI GTATCCnnnnn_n'
Cuts at: 0 487 903
Size: 487 416
BglI GCCn_nnn'nGGC
Cuts at: 0 500 841 903
Size: 500 341 62
Fragments arranged by size:
500 341 62
Bme1580I G_kGCm'C
Cuts at: 0 199 846 903
Size: 199 647 57
Fragments arranged by size:
647 199 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 86 679 903
Size: 86 593 224
Fragments arranged by size:
593 224 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 903
Size: 304 599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 903
Size: 152 751
BsaI GGTCTCn'nnnn_
Cuts at: 0 181 903
Size: 181 722
BsaWI w'CCGG_w
Cuts at: 0 7 903
Size: 7 896
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 903
Size: 344 559
BsiEI CG_ry'CG
Cuts at: 0 586 903
Size: 586 317
Bsp1286I G_dGCh'C
Cuts at: 0 199 846 903
Size: 199 647 57
Fragments arranged by size:
647 199 57
BsrFI r'CCGG_y
Cuts at: 0 199 395 903
Size: 199 196 508
Fragments arranged by size:
508 199 196
BsrGI T'GTAC_A
Cuts at: 0 128 903
Size: 128 775
BstAPI GCAn_nnn'nTGC
Cuts at: 0 358 832 903
Size: 358 474 71
Fragments arranged by size:
474 358 71
BtgI C'CryG_G
Cuts at: 0 332 510 903
Size: 332 178 393
Fragments arranged by size:
393 332 178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 366 401 903
Size: 366 35 502
Fragments arranged by size:
502 366 35
EarI CTCTTCn'nnn_
Cuts at: 0 247 903
Size: 247 656
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 304 903
Size: 175 129 599
Fragments arranged by size:
599 175 129
EcoNI CCTnn'n_nnAGG
Cuts at: 0 130 484 903
Size: 130 354 419
Fragments arranged by size:
419 354 130
EcoO109I rG'GnC_Cy
Cuts at: 0 135 338 622 903
Size: 135 203 284 281
Fragments arranged by size:
284 281 203 135
EcoRI G'AATT_C
Cuts at: 0 707 903
Size: 707 196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 150 182 903
Size: 150 32 721
Fragments arranged by size:
721 150 32
KpnI G_GTAC'C
Cuts at: 0 755 903
Size: 755 148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 872 903
Size: 872 31
MspA1I CmG'CkG
Cuts at: 0 43 334 903
Size: 43 291 569
Fragments arranged by size:
569 291 43
NaeI GCC'GGC
Cuts at: 0 397 903
Size: 397 506
NcoI C'CATG_G
Cuts at: 0 510 903
Size: 510 393
NgoMIV G'CCGG_C
Cuts at: 0 395 903
Size: 395 508
PpuMI rG'GwC_Cy
Cuts at: 0 338 903
Size: 338 565
PstI C_TGCA'G
Cuts at: 0 330 903
Size: 330 573
PvuI CG_AT'CG
Cuts at: 0 586 903
Size: 586 317
SacII CC_GC'GG
Cuts at: 0 335 903
Size: 335 568
ScaI AGT'ACT
Cuts at: 0 525 903
Size: 525 378
SfcI C'TryA_G
Cuts at: 0 326 400 903
Size: 326 74 503
Fragments arranged by size:
503 326 74
SmlI C'TyrA_G
Cuts at: 0 167 519 759 903
Size: 167 352 240 144
Fragments arranged by size:
352 240 167 144
StyI C'CwwG_G
Cuts at: 0 497 510 903
Size: 497 13 393
Fragments arranged by size:
497 393 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 225 903
Size: 225 678
TatI w'GTAC_w
Cuts at: 0 69 128 523 903
Size: 69 59 395 380
Fragments arranged by size:
395 380 69 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 51 83 903
Size: 51 32 820
Fragments arranged by size:
820 51 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 414 903
Size: 414 489
XhoI C'TCGA_G
Cuts at: 0 519 903
Size: 519 384
Enzymes that do cut:
Acc65I AcuI AflII ApoI AvaI BanI BbsI BciVI
BglI Bme1580I BmrI BpmI BpuEI BsaI BsaWI BsgI
BsiEI Bsp1286I BsrFI BsrGI BstAPI BtgI CspCI EarI
Eco57MI EcoNI EcoO109I EcoRI Hin4I KpnI MmeI MspA1I
NaeI NcoI NgoMIV PpuMI PstI PvuI SacII ScaI
SfcI SmlI StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BcgI
BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI MfeI MluI MscI MslI NarI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuII RsrII SacI SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XmaI XmnI ZraI