Restriction map for
pFN21AE6289
(Linear) (Six-base) MAPSORT of: KIEE6289.seq Check: 9816 from: 1 to: 918
KIEE6289.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AclI AA'CG_TT
Cuts at: 0 117 918
Size: 117 801
AflII C'TTAA_G
Cuts at: 0 829 918
Size: 829 89
AflIII A'CryG_T
Cuts at: 0 620 918
Size: 620 298
AgeI A'CCGG_T
Cuts at: 0 677 918
Size: 677 241
AlwNI CAG_nnn'CTG
Cuts at: 0 663 918
Size: 663 255
ApoI r'AATT_y
Cuts at: 0 145 918
Size: 145 773
BanI G'GyrC_C
Cuts at: 0 807 918
Size: 807 111
BanII G_rGCy'C
Cuts at: 0 572 918
Size: 572 346
BbsI GAAGACnn'nnnn_
Cuts at: 0 346 918
Size: 346 572
BbvCI CC'TCA_GC
Cuts at: 0 854 908 918
Size: 854 54 10
Fragments arranged by size:
854 54 10
BfrBI ATG'CAT
Cuts at: 0 783 918
Size: 783 135
Bme1580I G_kGCm'C
Cuts at: 0 810 918
Size: 810 108
BmtI G_CTAG'C
Cuts at: 0 916 918
Size: 916 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 567 748 795 918
Size: 188 379 181 47 123
Fragments arranged by size:
379 188 181 123 47
Bpu10I CC'TnA_GC
Cuts at: 0 422 815 854 908 918
Size: 422 393 39 54 10
Fragments arranged by size:
422 393 54 39 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 918
Size: 2 916
BsaWI w'CCGG_w
Cuts at: 0 365 677 918
Size: 365 312 241
Fragments arranged by size:
365 312 241
BsiWI C'GTAC_G
Cuts at: 0 639 918
Size: 639 279
BsmI GAATG_Cn'
Cuts at: 0 785 918
Size: 785 133
Bsp1286I G_dGCh'C
Cuts at: 0 572 810 918
Size: 572 238 108
Fragments arranged by size:
572 238 108
BspEI T'CCGG_A
Cuts at: 0 365 918
Size: 365 553
BsrFI r'CCGG_y
Cuts at: 0 677 686 918
Size: 677 9 232
Fragments arranged by size:
677 232 9
BsrGI T'GTAC_A
Cuts at: 0 238 918
Size: 238 680
BstBI TT'CG_AA
Cuts at: 0 743 918
Size: 743 175
BstXI CCAn_nnnn'nTGG
Cuts at: 0 231 918
Size: 231 687
Bsu36I CC'TnA_GG
Cuts at: 0 272 918
Size: 272 646
BtgI C'CryG_G
Cuts at: 0 802 918
Size: 802 116
DraI TTT'AAA
Cuts at: 0 150 396 918
Size: 150 246 522
Fragments arranged by size:
522 246 150
EaeI y'GGCC_r
Cuts at: 0 627 665 684 918
Size: 627 38 19 234
Fragments arranged by size:
627 234 38 19
EarI CTCTTCn'nnn_
Cuts at: 0 875 918
Size: 875 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 567 748 795 918
Size: 188 379 181 47 123
Fragments arranged by size:
379 188 181 123 47
EcoRV GAT'ATC
Cuts at: 0 429 918
Size: 429 489
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 447 463 918
Size: 447 16 455
Fragments arranged by size:
455 447 16
HincII GTy'rAC
Cuts at: 0 755 918
Size: 755 163
MscI TGG'CCA
Cuts at: 0 667 918
Size: 667 251
MspA1I CmG'CkG
Cuts at: 0 244 545 858 918
Size: 244 301 313 60
Fragments arranged by size:
313 301 244 60
NcoI C'CATG_G
Cuts at: 0 802 918
Size: 802 116
NheI G'CTAG_C
Cuts at: 0 912 918
Size: 912 6
NsiI A_TGCA'T
Cuts at: 0 785 918
Size: 785 133
NspI r_CATG'y
Cuts at: 0 178 648 918
Size: 178 470 270
Fragments arranged by size:
470 270 178
PfoI T'CCnGG_A
Cuts at: 0 725 918
Size: 725 193
PvuII CAG'CTG
Cuts at: 0 244 858 918
Size: 244 614 60
Fragments arranged by size:
614 244 60
RsrII CG'GwC_CG
Cuts at: 0 216 918
Size: 216 702
SfcI C'TryA_G
Cuts at: 0 92 918
Size: 92 826
SmlI C'TyrA_G
Cuts at: 0 17 829 918
Size: 17 812 89
Fragments arranged by size:
812 89 17
SphI G_CATG'C
Cuts at: 0 648 918
Size: 648 270
StyI C'CwwG_G
Cuts at: 0 485 716 802 918
Size: 485 231 86 116
Fragments arranged by size:
485 231 116 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 488 918
Size: 488 430
TatI w'GTAC_w
Cuts at: 0 238 918
Size: 238 680
Tth111I GACn'n_nGTC
Cuts at: 0 81 918
Size: 81 837
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 515 723 918
Size: 515 208 195
Fragments arranged by size:
515 208 195
Enzymes that do cut:
AclI AflII AflIII AgeI AlwNI ApoI BanI BanII
BbsI BbvCI BfrBI Bme1580I BmtI BpmI Bpu10I BpuEI
BsaWI BsiWI BsmI Bsp1286I BspEI BsrFI BsrGI BstBI
BstXI Bsu36I BtgI DraI EaeI EarI Eco57MI EcoRV
FalI HincII MscI MspA1I NcoI NheI NsiI NspI
PfoI PvuII RsrII SfcI SmlI SphI StyI TaqII
TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BglI
BglII BlpI BmgBI BmrI BplI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstEII
BstYI BstZ17I BtgZI BtsI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI FseI FspI
FspAI HaeII Hin4I HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NaeI NarI NdeI NgoMIV NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI SwaI TstI XbaI XhoI XmaI XmnI
ZraI