Restriction map for
pFN21AE6287
(Linear) (Six-base) MAPSORT of: KIEE6287.seq Check: 5682 from: 1 to: 888
KIEE6287.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 888
Size: 567 321
AflIII A'CryG_T
Cuts at: 0 596 888
Size: 596 292
AlwNI CAG_nnn'CTG
Cuts at: 0 351 705 888
Size: 351 354 183
Fragments arranged by size:
354 351 183
ApaI G_GGCC'C
Cuts at: 0 306 888
Size: 306 582
AvaI C'yCGr_G
Cuts at: 0 68 888
Size: 68 820
BanII G_rGCy'C
Cuts at: 0 152 306 888
Size: 152 154 582
Fragments arranged by size:
582 154 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 573 888
Size: 573 315
BbvCI CC'TCA_GC
Cuts at: 0 640 888
Size: 640 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 87 121 888
Size: 87 34 767
Fragments arranged by size:
767 87 34
BfrBI ATG'CAT
Cuts at: 0 568 888
Size: 568 320
Bme1580I G_kGCm'C
Cuts at: 0 11 306 888
Size: 11 295 582
Fragments arranged by size:
582 295 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 433 888
Size: 433 455
Bpu10I CC'TnA_GC
Cuts at: 0 640 888
Size: 640 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 857 888
Size: 857 31
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 258 888
Size: 258 630
BseYI C'CCAG_C
Cuts at: 0 171 888
Size: 171 717
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 888
Size: 839 49
BsiEI CG_ry'CG
Cuts at: 0 101 888
Size: 101 787
BsiHKAI G_wGCw'C
Cuts at: 0 56 888
Size: 56 832
BsmI GAATG_Cn'
Cuts at: 0 88 888
Size: 88 800
BsmBI CGTCTCn'nnnn_
Cuts at: 0 226 888
Size: 226 662
Bsp1286I G_dGCh'C
Cuts at: 0 11 56 152 306 888
Size: 11 45 96 154 582
Fragments arranged by size:
582 154 96 45 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 176 888
Size: 176 712
BsrBI CCG'CTC
Cuts at: 0 67 888
Size: 67 821
BsrDI GCAATG_nn'
Cuts at: 0 683 888
Size: 683 205
BstAPI GCAn_nnn'nTGC
Cuts at: 0 705 888
Size: 705 183
BstYI r'GATC_y
Cuts at: 0 855 888
Size: 855 33
Bsu36I CC'TnA_GG
Cuts at: 0 136 888
Size: 136 752
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 777 888
Size: 777 111
BtsI GCAGTG_nn'
Cuts at: 0 530 694 888
Size: 530 164 194
Fragments arranged by size:
530 194 164
EaeI y'GGCC_r
Cuts at: 0 44 809 888
Size: 44 765 79
Fragments arranged by size:
765 79 44
EarI CTCTTCn'nnn_
Cuts at: 0 284 888
Size: 284 604
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 888
Size: 567 321
EcoO109I rG'GnC_Cy
Cuts at: 0 303 348 888
Size: 303 45 540
Fragments arranged by size:
540 303 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 357 389 480 512 847 879 888
Size: 357 32 91 32 335 32 9
Fragments arranged by size:
357 335 91 32 32 32 9
HincII GTy'rAC
Cuts at: 0 473 888
Size: 473 415
MscI TGG'CCA
Cuts at: 0 811 888
Size: 811 77
MslI CAynn'nnrTG
Cuts at: 0 556 595 796 888
Size: 556 39 201 92
Fragments arranged by size:
556 201 92 39
NruI TCG'CGA
Cuts at: 0 80 888
Size: 80 808
NsiI A_TGCA'T
Cuts at: 0 570 888
Size: 570 318
NspI r_CATG'y
Cuts at: 0 600 888
Size: 600 288
PciI A'CATG_T
Cuts at: 0 596 888
Size: 596 292
PspOMI G'GGCC_C
Cuts at: 0 302 888
Size: 302 586
PstI C_TGCA'G
Cuts at: 0 525 701 888
Size: 525 176 187
Fragments arranged by size:
525 187 176
RsrII CG'GwC_CG
Cuts at: 0 62 888
Size: 62 826
ScaI AGT'ACT
Cuts at: 0 239 888
Size: 239 649
SfcI C'TryA_G
Cuts at: 0 521 697 888
Size: 521 176 191
Fragments arranged by size:
521 191 176
SmlI C'TyrA_G
Cuts at: 0 872 888
Size: 872 16
StyI C'CwwG_G
Cuts at: 0 738 888
Size: 738 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 208 415 546 888
Size: 208 207 131 342
Fragments arranged by size:
342 208 207 131
TatI w'GTAC_w
Cuts at: 0 237 888
Size: 237 651
XmnI GAAnn'nnTTC
Cuts at: 0 250 888
Size: 250 638
Enzymes that do cut:
AcuI AflIII AlwNI ApaI AvaI BanII BbsI BbvCI
BcgI BfrBI Bme1580I BmrI Bpu10I BpuEI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BstAPI BstYI Bsu36I BtgZI BtsI EaeI EarI
Eco57MI EcoO109I Hin4I HincII MscI MslI NruI NsiI
NspI PciI PspOMI PstI RsrII ScaI SfcI SmlI
StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BciVI BclI
BglI BglII BlpI BmgBI BmtI BplI BpmI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiWI BspEI BspHI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI