(Linear) (Six-base) MAPSORT of: KIEE6286.seq  Check: 5088  from: 1  to: 870



KIEE6286.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:29  ..

AccI GT'mk_AC

Cuts at:      0     730     870 
   Size:        730     140
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     488     870 
   Size:        488     382
 
AhdI GACnn_n'nnGTC

Cuts at:      0     541     870 
   Size:        541     329
 
ApoI r'AATT_y

Cuts at:      0     797     870 
   Size:        797      73
 
AseI AT'TA_AT

Cuts at:      0     424     443     870 
   Size:        424      19     427

  Fragments arranged by size: 

                 427     424      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     476     870 
   Size:        476     394
 
BfrBI ATG'CAT

Cuts at:      0     339     870 
   Size:        339     531
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     605     870 
   Size:        605     265
 
BsaBI GATnn'nnATC

Cuts at:      0     338     870 
   Size:        338     532
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     222     252     870 
   Size:        222      30     618

  Fragments arranged by size: 

                 618     222      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     208     489     870 
   Size:        208     281     381

  Fragments arranged by size: 

                 381     281     208
 
BsiHKAI G_wGCw'C

Cuts at:      0     240     870 
   Size:        240     630
 
BsmI GAATG_Cn'

Cuts at:      0     768     870 
   Size:        768     102
 
Bsp1286I G_dGCh'C

Cuts at:      0     240     870 
   Size:        240     630
 
BstZ17I GTA'TAC

Cuts at:      0     731     870 
   Size:        731     139
 
DraI TTT'AAA

Cuts at:      0     117     870 
   Size:        117     753
 
EaeI y'GGCC_r

Cuts at:      0     749     870 
   Size:        749     121
 
EarI CTCTTCn'nnn_

Cuts at:      0      63     851     870 
   Size:         63     788      19

  Fragments arranged by size: 

                 788      63      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     488     605     870 
   Size:        488     117     265

  Fragments arranged by size: 

                 488     265     117
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     527     559     870 
   Size:        527      32     311

  Fragments arranged by size: 

                 527     311      32
 
MfeI C'AATT_G

Cuts at:      0     451     870 
   Size:        451     419
 
MscI TGG'CCA

Cuts at:      0     751     870 
   Size:        751     119
 
NdeI CA'TA_TG

Cuts at:      0     809     870 
   Size:        809      61
 
NsiI A_TGCA'T

Cuts at:      0     341     870 
   Size:        341     529
 
SfcI C'TryA_G

Cuts at:      0     362     370     870 
   Size:        362       8     500

  Fragments arranged by size: 

                 500     362       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     219     251     870 
   Size:        219      32     619

  Fragments arranged by size: 

                 619     219      32
 

 Enzymes that do cut:

     AccI     AcuI     AhdI     ApoI     AseI     BbsI    BfrBI     BpmI
    BsaBI    BsaXI    BseRI  BsiHKAI     BsmI Bsp1286I  BstZ17I     DraI
     EaeI     EarI  Eco57MI    Hin4I     MfeI     MscI     NdeI     NsiI
     SfcI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI