Restriction map for
pFN21AE6281
(Linear) (Six-base) MAPSORT of: KIEE6281.seq Check: 3191 from: 1 to: 756
KIEE6281.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AccI GT'mk_AC
Cuts at: 0 653 756
Size: 653 103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 756
Size: 377 379
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 83 115 756
Size: 83 32 641
Fragments arranged by size:
641 83 32
AlwNI CAG_nnn'CTG
Cuts at: 0 415 756
Size: 415 341
AvrII C'CTAG_G
Cuts at: 0 154 756
Size: 154 602
BamHI G'GATC_C
Cuts at: 0 181 756
Size: 181 575
BanII G_rGCy'C
Cuts at: 0 712 756
Size: 712 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 365 756
Size: 365 391
BbvCI CC'TCA_GC
Cuts at: 0 19 756
Size: 19 737
Bme1580I G_kGCm'C
Cuts at: 0 693 756
Size: 693 63
BmgBI CAC'GTC
Cuts at: 0 456 756
Size: 456 300
BmrI ACTGGGnnnn_n'
Cuts at: 0 425 756
Size: 425 331
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 483 756
Size: 483 273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 726 756
Size: 351 375 30
Fragments arranged by size:
375 351 30
Bpu10I CC'TnA_GC
Cuts at: 0 19 756
Size: 19 737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 756
Size: 238 518
BsaWI w'CCGG_w
Cuts at: 0 712 756
Size: 712 44
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 259 541 756
Size: 52 207 282 215
Fragments arranged by size:
282 215 207 52
BseYI C'CCAG_C
Cuts at: 0 48 756
Size: 48 708
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 756
Size: 381 375
BsiEI CG_ry'CG
Cuts at: 0 83 87 756
Size: 83 4 669
Fragments arranged by size:
669 83 4
BsiHKAI G_wGCw'C
Cuts at: 0 339 712 756
Size: 339 373 44
Fragments arranged by size:
373 339 44
BsmI GAATG_Cn'
Cuts at: 0 285 315 756
Size: 285 30 441
Fragments arranged by size:
441 285 30
Bsp1286I G_dGCh'C
Cuts at: 0 339 693 712 756
Size: 339 354 19 44
Fragments arranged by size:
354 339 44 19
BspEI T'CCGG_A
Cuts at: 0 712 756
Size: 712 44
BsrBI CCG'CTC
Cuts at: 0 521 756
Size: 521 235
BstEII G'GTnAC_C
Cuts at: 0 289 756
Size: 289 467
BstYI r'GATC_y
Cuts at: 0 181 756
Size: 181 575
BtgI C'CryG_G
Cuts at: 0 284 647 756
Size: 284 363 109
Fragments arranged by size:
363 284 109
EaeI y'GGCC_r
Cuts at: 0 80 296 756
Size: 80 216 460
Fragments arranged by size:
460 216 80
EagI C'GGCC_G
Cuts at: 0 80 756
Size: 80 676
EarI CTCTTCn'nnn_
Cuts at: 0 637 756
Size: 637 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 39 541 756
Size: 39 502 215
Fragments arranged by size:
502 215 39
EcoICRI GAG'CTC
Cuts at: 0 710 756
Size: 710 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 377 726 756
Size: 351 26 349 30
Fragments arranged by size:
351 349 30 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 238 756
Size: 238 518
EcoO109I rG'GnC_Cy
Cuts at: 0 72 756
Size: 72 684
FspI TGC'GCA
Cuts at: 0 697 756
Size: 697 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 44 76 756
Size: 44 32 680
Fragments arranged by size:
680 44 32
MscI TGG'CCA
Cuts at: 0 298 756
Size: 298 458
MspA1I CmG'CkG
Cuts at: 0 9 192 316 545 732 756
Size: 9 183 124 229 187 24
Fragments arranged by size:
229 187 183 124 24 9
NcoI C'CATG_G
Cuts at: 0 647 756
Size: 647 109
NdeI CA'TA_TG
Cuts at: 0 747 756
Size: 747 9
NspI r_CATG'y
Cuts at: 0 540 756
Size: 540 216
PpuMI rG'GwC_Cy
Cuts at: 0 72 756
Size: 72 684
PvuII CAG'CTG
Cuts at: 0 9 192 732 756
Size: 9 183 540 24
Fragments arranged by size:
540 183 24 9
SacI G_AGCT'C
Cuts at: 0 712 756
Size: 712 44
SmlI C'TyrA_G
Cuts at: 0 253 756
Size: 253 503
SphI G_CATG'C
Cuts at: 0 540 756
Size: 540 216
StuI AGG'CCT
Cuts at: 0 532 756
Size: 532 224
StyI C'CwwG_G
Cuts at: 0 154 239 579 647 756
Size: 154 85 340 68 109
Fragments arranged by size:
340 154 109 85 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 101 756
Size: 101 655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 291 756
Size: 291 465
Enzymes that do cut:
AccI AcuI AloI AlwNI AvrII BamHI BanII BbsI
BbvCI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BsrBI BstEII BstYI BtgI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I MscI
MspA1I NcoI NdeI NspI PpuMI PvuII SacI SmlI
SphI StuI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BaeI BanI BbeI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmtI BsaI BsaAI BsaBI
BsaHI BsaXI BsiWI BsmBI BspHI BspMI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoRI
EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MslI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI