Restriction map for
pFN21AE6279
(Linear) (Six-base) MAPSORT of: KIEE6279.seq Check: 4445 from: 1 to: 726
KIEE6279.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 250 726
Size: 250 476
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 726
Size: 124 602
AleI CACnn'nnGTG
Cuts at: 0 208 726
Size: 208 518
ApaI G_GGCC'C
Cuts at: 0 483 726
Size: 483 243
AvaI C'yCGr_G
Cuts at: 0 37 432 503 726
Size: 37 395 71 223
Fragments arranged by size:
395 223 71 37
BanI G'GyrC_C
Cuts at: 0 33 98 726
Size: 33 65 628
Fragments arranged by size:
628 65 33
BanII G_rGCy'C
Cuts at: 0 483 561 726
Size: 483 78 165
Fragments arranged by size:
483 165 78
BbeI G_GCGC'C
Cuts at: 0 37 102 726
Size: 37 65 624
Fragments arranged by size:
624 65 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 522 726
Size: 522 204
BbvCI CC'TCA_GC
Cuts at: 0 154 726
Size: 154 572
BglI GCCn_nnn'nGGC
Cuts at: 0 88 505 726
Size: 88 417 221
Fragments arranged by size:
417 221 88
Bme1580I G_kGCm'C
Cuts at: 0 353 483 726
Size: 353 130 243
Fragments arranged by size:
353 243 130
BmrI ACTGGGnnnn_n'
Cuts at: 0 638 726
Size: 638 88
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 726
Size: 367 359
Bpu10I CC'TnA_GC
Cuts at: 0 154 726
Size: 154 572
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 726
Size: 322 404
BsaI GGTCTCn'nnnn_
Cuts at: 0 241 537 726
Size: 241 296 189
Fragments arranged by size:
296 241 189
BsaHI Gr'CG_yC
Cuts at: 0 34 99 726
Size: 34 65 627
Fragments arranged by size:
627 65 34
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 678 726
Size: 678 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 459 726
Size: 340 119 267
Fragments arranged by size:
340 267 119
BsiHKAI G_wGCw'C
Cuts at: 0 230 726
Size: 230 496
BsmI GAATG_Cn'
Cuts at: 0 144 726
Size: 144 582
Bsp1286I G_dGCh'C
Cuts at: 0 230 353 483 561 726
Size: 230 123 130 78 165
Fragments arranged by size:
230 165 130 123 78
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 250 726
Size: 250 476
BsrDI GCAATG_nn'
Cuts at: 0 609 726
Size: 609 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 726
Size: 210 516
BtgI C'CryG_G
Cuts at: 0 29 56 538 726
Size: 29 27 482 188
Fragments arranged by size:
482 188 29 27
BtsI GCAGTG_nn'
Cuts at: 0 203 353 726
Size: 203 150 373
Fragments arranged by size:
373 203 150
EarI CTCTTCn'nnn_
Cuts at: 0 262 301 726
Size: 262 39 425
Fragments arranged by size:
425 262 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 235 460 726
Size: 235 225 266
Fragments arranged by size:
266 235 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 367 726
Size: 124 243 359
Fragments arranged by size:
359 243 124
EcoNI CCTnn'n_nnAGG
Cuts at: 0 304 726
Size: 304 422
EcoO109I rG'GnC_Cy
Cuts at: 0 480 657 679 726
Size: 480 177 22 47
Fragments arranged by size:
480 177 47 22
HaeII r_GCGC'y
Cuts at: 0 37 102 726
Size: 37 65 624
Fragments arranged by size:
624 65 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 534 566 726
Size: 534 32 160
Fragments arranged by size:
534 160 32
KasI G'GCGC_C
Cuts at: 0 33 98 726
Size: 33 65 628
Fragments arranged by size:
628 65 33
MslI CAynn'nnrTG
Cuts at: 0 208 726
Size: 208 518
MspA1I CmG'CkG
Cuts at: 0 7 31 58 412 526 726
Size: 7 24 27 354 114 200
Fragments arranged by size:
354 200 114 27 24 7
NarI GG'CG_CC
Cuts at: 0 34 99 726
Size: 34 65 627
Fragments arranged by size:
627 65 34
PpuMI rG'GwC_Cy
Cuts at: 0 657 726
Size: 657 69
PshAI GACnn'nnGTC
Cuts at: 0 662 726
Size: 662 64
PspOMI G'GGCC_C
Cuts at: 0 479 726
Size: 479 247
PstI C_TGCA'G
Cuts at: 0 656 726
Size: 656 70
PvuII CAG'CTG
Cuts at: 0 412 526 726
Size: 412 114 200
Fragments arranged by size:
412 200 114
SacII CC_GC'GG
Cuts at: 0 32 59 726
Size: 32 27 667
Fragments arranged by size:
667 32 27
SapI GCTCTTCn'nnn_
Cuts at: 0 262 726
Size: 262 464
SexAI A'CCwGG_T
Cuts at: 0 287 726
Size: 287 439
SfcI C'TryA_G
Cuts at: 0 652 726
Size: 652 74
SfoI GGC'GCC
Cuts at: 0 35 100 726
Size: 35 65 626
Fragments arranged by size:
626 65 35
SmaI CCC'GGG
Cuts at: 0 39 505 726
Size: 39 466 221
Fragments arranged by size:
466 221 39
SmlI C'TyrA_G
Cuts at: 0 337 726
Size: 337 389
SrfI GCCC'GGGC
Cuts at: 0 39 726
Size: 39 687
TatI w'GTAC_w
Cuts at: 0 107 726
Size: 107 619
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 69 101 726
Size: 69 32 625
Fragments arranged by size:
625 69 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 726
Size: 207 519
XmaI C'CCGG_G
Cuts at: 0 37 503 726
Size: 37 466 223
Fragments arranged by size:
466 223 37
Enzymes that do cut:
AarI AcuI AleI ApaI AvaI BanI BanII BbeI
BbsI BbvCI BglI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaHI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BstXI BtgI BtsI EarI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MslI MspA1I NarI PpuMI
PshAI PspOMI PstI PvuII SacII SapI SexAI SfcI
SfoI SmaI SmlI SrfI TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsaWI
BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI
SalI SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII Tth111I
XbaI XhoI XmnI ZraI