(Linear) (Six-base) MAPSORT of: KIEE6276.seq  Check: 8367  from: 1  to: 660



KIEE6276.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:29  ..

AccI GT'mk_AC

Cuts at:      0     173     413     660 
   Size:        173     240     247

  Fragments arranged by size: 

                 247     240     173
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     660 
   Size:        271     389
 
AlwNI CAG_nnn'CTG

Cuts at:      0     423     660 
   Size:        423     237
 
ApoI r'AATT_y

Cuts at:      0      32     227     359     660 
   Size:         32     195     132     301

  Fragments arranged by size: 

                 301     195     132      32
 
BsaAI yAC'GTr

Cuts at:      0     347     660 
   Size:        347     313
 
BseYI C'CCAG_C

Cuts at:      0     565     660 
   Size:        565      95
 
BsiHKAI G_wGCw'C

Cuts at:      0     204     660 
   Size:        204     456
 
Bsp1286I G_dGCh'C

Cuts at:      0     204     660 
   Size:        204     456
 
BsrGI T'GTAC_A

Cuts at:      0     459     660 
   Size:        459     201
 
BstYI r'GATC_y

Cuts at:      0     275     660 
   Size:        275     385
 
BstZ17I GTA'TAC

Cuts at:      0     414     660 
   Size:        414     246
 
BtsI GCAGTG_nn'

Cuts at:      0     131     660 
   Size:        131     529
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     592     627     660 
   Size:        592      35      33

  Fragments arranged by size: 

                 592      35      33
 
DraI TTT'AAA

Cuts at:      0      89     660 
   Size:         89     571
 
EarI CTCTTCn'nnn_

Cuts at:      0     290     660 
   Size:        290     370
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     660 
   Size:        271     389
 
EcoO109I rG'GnC_Cy

Cuts at:      0     100     660 
   Size:        100     560
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     267     299     660 
   Size:        267      32     361

  Fragments arranged by size: 

                 361     267      32
 
MspA1I CmG'CkG

Cuts at:      0     505     660 
   Size:        505     155
 
NspI r_CATG'y

Cuts at:      0     548     660 
   Size:        548     112
 
PasI CC'CwG_GG

Cuts at:      0      27     660 
   Size:         27     633
 
PpuMI rG'GwC_Cy

Cuts at:      0     100     660 
   Size:        100     560
 
PvuII CAG'CTG

Cuts at:      0     505     660 
   Size:        505     155
 
ScaI AGT'ACT

Cuts at:      0     215     660 
   Size:        215     445
 
SfcI C'TryA_G

Cuts at:      0     119     660 
   Size:        119     541
 
SspI AAT'ATT

Cuts at:      0     624     660 
   Size:        624      36
 
StyI C'CwwG_G

Cuts at:      0     644     660 
   Size:        644      16
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     397     660 
   Size:        397     263
 
TatI w'GTAC_w

Cuts at:      0     213     459     660 
   Size:        213     246     201

  Fragments arranged by size: 

                 246     213     201
 
XbaI T'CTAG_A

Cuts at:      0     321     660 
   Size:        321     339
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI    BsaAI    BseYI  BsiHKAI Bsp1286I
    BsrGI    BstYI  BstZ17I     BtsI    CspCI     DraI     EarI  Eco57MI
 EcoO109I    Hin4I   MspA1I     NspI     PasI    PpuMI    PvuII     ScaI
     SfcI     SspI     StyI    TaqII     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
   Bsu36I     BtgI    BtgZI     ClaI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI
  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI