Restriction map for
pFN21AE6274
(Linear) (Six-base) MAPSORT of: KIEE6274.seq Check: 1027 from: 1 to: 570
KIEE6274.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 570
Size: 162 408
AgeI A'CCGG_T
Cuts at: 0 360 570
Size: 360 210
AhdI GACnn_n'nnGTC
Cuts at: 0 303 570
Size: 303 267
AleI CACnn'nnGTG
Cuts at: 0 274 570
Size: 274 296
ApaI G_GGCC'C
Cuts at: 0 96 264 570
Size: 96 168 306
Fragments arranged by size:
306 168 96
ApoI r'AATT_y
Cuts at: 0 412 570
Size: 412 158
BamHI G'GATC_C
Cuts at: 0 201 570
Size: 201 369
BanII G_rGCy'C
Cuts at: 0 96 226 264 570
Size: 96 130 38 306
Fragments arranged by size:
306 130 96 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 431 570
Size: 397 34 139
Fragments arranged by size:
397 139 34
Bme1580I G_kGCm'C
Cuts at: 0 96 264 570
Size: 96 168 306
Fragments arranged by size:
306 168 96
BmgBI CAC'GTC
Cuts at: 0 120 570
Size: 120 450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 415 570
Size: 229 186 155
Fragments arranged by size:
229 186 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 570
Size: 328 242
BsaAI yAC'GTr
Cuts at: 0 483 570
Size: 483 87
BsaBI GATnn'nnATC
Cuts at: 0 25 570
Size: 25 545
BsaWI w'CCGG_w
Cuts at: 0 360 570
Size: 360 210
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 570
Size: 64 506
BseYI C'CCAG_C
Cuts at: 0 227 509 570
Size: 227 282 61
Fragments arranged by size:
282 227 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 535 570
Size: 100 435 35
Fragments arranged by size:
435 100 35
BsiHKAI G_wGCw'C
Cuts at: 0 317 570
Size: 317 253
BsmBI CGTCTCn'nnnn_
Cuts at: 0 430 570
Size: 430 140
Bsp1286I G_dGCh'C
Cuts at: 0 96 226 264 317 570
Size: 96 130 38 53 253
Fragments arranged by size:
253 130 96 53 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 293 510 570
Size: 293 217 60
Fragments arranged by size:
293 217 60
BsrBI CCG'CTC
Cuts at: 0 42 219 570
Size: 42 177 351
Fragments arranged by size:
351 177 42
BsrFI r'CCGG_y
Cuts at: 0 360 543 570
Size: 360 183 27
Fragments arranged by size:
360 183 27
BstYI r'GATC_y
Cuts at: 0 201 374 570
Size: 201 173 196
Fragments arranged by size:
201 196 173
BtsI GCAGTG_nn'
Cuts at: 0 550 570
Size: 550 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 444 479 570
Size: 444 35 91
Fragments arranged by size:
444 91 35
DraIII CAC_nnn'GTG
Cuts at: 0 354 570
Size: 354 216
EaeI y'GGCC_r
Cuts at: 0 128 164 545 570
Size: 128 36 381 25
Fragments arranged by size:
381 128 36 25
EarI CTCTTCn'nnn_
Cuts at: 0 564 570
Size: 564 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 79 570
Size: 79 491
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 229 415 570
Size: 162 67 186 155
Fragments arranged by size:
186 162 155 67
EcoO109I rG'GnC_Cy
Cuts at: 0 260 261 522 570
Size: 260 1 261 48
Fragments arranged by size:
261 260 48 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 192 570
Size: 192 378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 289 321 570
Size: 40 32 217 32 249
Fragments arranged by size:
249 217 40 32 32
MscI TGG'CCA
Cuts at: 0 130 570
Size: 130 440
MslI CAynn'nnrTG
Cuts at: 0 136 274 570
Size: 136 138 296
Fragments arranged by size:
296 138 136
MspA1I CmG'CkG
Cuts at: 0 110 231 490 570
Size: 110 121 259 80
Fragments arranged by size:
259 121 110 80
PmlI CAC'GTG
Cuts at: 0 483 570
Size: 483 87
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 570
Size: 145 32 393
Fragments arranged by size:
393 145 32
PpuMI rG'GwC_Cy
Cuts at: 0 522 570
Size: 522 48
PspOMI G'GGCC_C
Cuts at: 0 92 260 570
Size: 92 168 310
Fragments arranged by size:
310 168 92
PvuII CAG'CTG
Cuts at: 0 110 231 570
Size: 110 121 339
Fragments arranged by size:
339 121 110
ScaI AGT'ACT
Cuts at: 0 175 570
Size: 175 395
SfcI C'TryA_G
Cuts at: 0 474 570
Size: 474 96
SmlI C'TyrA_G
Cuts at: 0 343 570
Size: 343 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 501 570
Size: 501 69
TatI w'GTAC_w
Cuts at: 0 173 365 570
Size: 173 192 205
Fragments arranged by size:
205 192 173
Enzymes that do cut:
AcuI AgeI AhdI AleI ApaI ApoI BamHI BanII
BcgI Bme1580I BmgBI BpmI BpuEI BsaAI BsaBI BsaWI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BstYI BtsI CspCI DraIII EaeI EarI EciI
Eco57MI EcoO109I FalI Hin4I MscI MslI MspA1I PmlI
PpiI PpuMI PspOMI PvuII ScaI SfcI SmlI TaqII
TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI BmrI BmtI BplI Bpu10I
BsaI BsaHI BsaXI BsiEI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI ClaI DraI DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI