(Linear) (Six-base) MAPSORT of: KIEE6271.seq  Check: 7510  from: 1  to: 546



KIEE6271.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:29  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     380     546 
   Size:         80     300     166

  Fragments arranged by size: 

                 300     166      80
 
ApoI r'AATT_y

Cuts at:      0     298     306     546 
   Size:        298       8     240

  Fragments arranged by size: 

                 298     240       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      42     546 
   Size:         42     504
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     459     546 
   Size:        459      87
 
BfrBI ATG'CAT

Cuts at:      0     270     546 
   Size:        270     276
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     546 
   Size:        337     209
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     343     546 
   Size:        343     203
 
BsaBI GATnn'nnATC

Cuts at:      0     139     546 
   Size:        139     407
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       1      10     546 
   Size:          1       9     536

  Fragments arranged by size: 

                 536       9       1
 
BsmI GAATG_Cn'

Cuts at:      0     243     546 
   Size:        243     303
 
BsrDI GCAATG_nn'

Cuts at:      0     270     546 
   Size:        270     276
 
BstYI r'GATC_y

Cuts at:      0     235     546 
   Size:        235     311
 
BtgI C'CryG_G

Cuts at:      0      88     546 
   Size:         88     458
 
DraI TTT'AAA

Cuts at:      0     512     546 
   Size:        512      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     337     380     546 
   Size:         80     257      43     166

  Fragments arranged by size: 

                 257     166      80      43
 
MfeI C'AATT_G

Cuts at:      0     149     546 
   Size:        149     397
 
MslI CAynn'nnrTG

Cuts at:      0     538     546 
   Size:        538       8
 
NsiI A_TGCA'T

Cuts at:      0     272     546 
   Size:        272     274
 
NspI r_CATG'y

Cuts at:      0      79     546 
   Size:         79     467
 
SspI AAT'ATT

Cuts at:      0      68     546 
   Size:         68     478
 
StyI C'CwwG_G

Cuts at:      0     282     546 
   Size:        282     264
 

 Enzymes that do cut:

     AcuI     ApoI     BbsI    BciVI    BfrBI     BpmI     BsaI    BsaBI
    BseRI     BsmI    BsrDI    BstYI     BtgI     DraI  Eco57MI     MfeI
     MslI     NsiI     NspI     SspI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI