Restriction map for
pFN21AE6270
(Linear) (Six-base) MAPSORT of: KIEE6270.seq Check: 998 from: 1 to: 576
KIEE6270.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 576
Size: 85 491
ApoI r'AATT_y
Cuts at: 0 246 364 517 576
Size: 246 118 153 59
Fragments arranged by size:
246 153 118 59
BanI G'GyrC_C
Cuts at: 0 13 576
Size: 13 563
BanII G_rGCy'C
Cuts at: 0 27 178 576
Size: 27 151 398
Fragments arranged by size:
398 151 27
BbeI G_GCGC'C
Cuts at: 0 17 576
Size: 17 559
BbsI GAAGACnn'nnnn_
Cuts at: 0 116 263 576
Size: 116 147 313
Fragments arranged by size:
313 147 116
BglI GCCn_nnn'nGGC
Cuts at: 0 22 576
Size: 22 554
BmgBI CAC'GTC
Cuts at: 0 447 576
Size: 447 129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 247 576
Size: 247 329
BsaI GGTCTCn'nnnn_
Cuts at: 0 221 576
Size: 221 355
BsaHI Gr'CG_yC
Cuts at: 0 14 576
Size: 14 562
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 239 576
Size: 239 337
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 576
Size: 113 463
BsiHKAI G_wGCw'C
Cuts at: 0 161 576
Size: 161 415
Bsp1286I G_dGCh'C
Cuts at: 0 27 161 178 576
Size: 27 134 17 398
Fragments arranged by size:
398 134 27 17
BspHI T'CATG_A
Cuts at: 0 134 339 576
Size: 134 205 237
Fragments arranged by size:
237 205 134
BsrFI r'CCGG_y
Cuts at: 0 210 576
Size: 210 366
BsrGI T'GTAC_A
Cuts at: 0 410 576
Size: 410 166
BstEII G'GTnAC_C
Cuts at: 0 489 576
Size: 489 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 186 576
Size: 186 390
BtgI C'CryG_G
Cuts at: 0 17 576
Size: 17 559
BtsI GCAGTG_nn'
Cuts at: 0 174 576
Size: 174 402
DraI TTT'AAA
Cuts at: 0 104 291 576
Size: 104 187 285
Fragments arranged by size:
285 187 104
EarI CTCTTCn'nnn_
Cuts at: 0 450 576
Size: 450 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 576
Size: 85 491
EcoO109I rG'GnC_Cy
Cuts at: 0 60 576
Size: 60 516
EcoRV GAT'ATC
Cuts at: 0 51 576
Size: 51 525
HaeII r_GCGC'y
Cuts at: 0 17 84 576
Size: 17 67 492
Fragments arranged by size:
492 67 17
KasI G'GCGC_C
Cuts at: 0 13 576
Size: 13 563
MslI CAynn'nnrTG
Cuts at: 0 118 184 499 576
Size: 118 66 315 77
Fragments arranged by size:
315 118 77 66
NaeI GCC'GGC
Cuts at: 0 212 576
Size: 212 364
NarI GG'CG_CC
Cuts at: 0 14 576
Size: 14 562
NdeI CA'TA_TG
Cuts at: 0 477 552 576
Size: 477 75 24
Fragments arranged by size:
477 75 24
NgoMIV G'CCGG_C
Cuts at: 0 210 576
Size: 210 366
PflMI CCAn_nnn'nTGG
Cuts at: 0 481 576
Size: 481 95
PfoI T'CCnGG_A
Cuts at: 0 251 576
Size: 251 325
PpuMI rG'GwC_Cy
Cuts at: 0 60 576
Size: 60 516
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 320 352 576
Size: 320 32 224
Fragments arranged by size:
320 224 32
SapI GCTCTTCn'nnn_
Cuts at: 0 450 576
Size: 450 126
SexAI A'CCwGG_T
Cuts at: 0 95 576
Size: 95 481
SfoI GGC'GCC
Cuts at: 0 15 576
Size: 15 561
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 535 576
Size: 535 41
TatI w'GTAC_w
Cuts at: 0 410 576
Size: 410 166
Enzymes that do cut:
AcuI ApoI BanI BanII BbeI BbsI BglI BmgBI
BplI BsaI BsaHI BseRI BsgI BsiHKAI Bsp1286I BspHI
BsrFI BsrGI BstEII BstXI BtgI BtsI DraI EarI
Eco57MI EcoO109I EcoRV HaeII KasI MslI NaeI NarI
NdeI NgoMIV PflMI PfoI PpuMI PsrI SapI SexAI
SfoI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrDI BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MspA1I NcoI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI