Restriction map for
pFN21AE6269
(Linear) (Six-base) MAPSORT of: KIEE6269.seq Check: 9344 from: 1 to: 765
KIEE6269.orf
With 182 enzymes: SgfI *
March 16, 2015 11:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 44 429 765
Size: 44 385 336
Fragments arranged by size:
385 336 44
ApaI G_GGCC'C
Cuts at: 0 532 589 682 765
Size: 532 57 93 83
Fragments arranged by size:
532 93 83 57
ApoI r'AATT_y
Cuts at: 0 13 765
Size: 13 752
AvaI C'yCGr_G
Cuts at: 0 337 349 765
Size: 337 12 416
Fragments arranged by size:
416 337 12
BanI G'GyrC_C
Cuts at: 0 137 505 765
Size: 137 368 260
Fragments arranged by size:
368 260 137
BanII G_rGCy'C
Cuts at: 0 115 215 532 589 682 765
Size: 115 100 317 57 93 83
Fragments arranged by size:
317 115 100 93 83 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 526 745 765
Size: 526 219 20
Fragments arranged by size:
526 219 20
BfrBI ATG'CAT
Cuts at: 0 400 500 765
Size: 400 100 265
Fragments arranged by size:
400 265 100
Bme1580I G_kGCm'C
Cuts at: 0 532 589 682 765
Size: 532 57 93 83
Fragments arranged by size:
532 93 83 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 179 330 765
Size: 179 151 435
Fragments arranged by size:
435 179 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 765
Size: 617 148
BsaI GGTCTCn'nnnn_
Cuts at: 0 330 765
Size: 330 435
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 301 397 765
Size: 301 96 368
Fragments arranged by size:
368 301 96
BseYI C'CCAG_C
Cuts at: 0 108 228 580 675 765
Size: 108 120 352 95 90
Fragments arranged by size:
352 120 108 95 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 765
Size: 106 659
BsmI GAATG_Cn'
Cuts at: 0 402 765
Size: 402 363
Bsp1286I G_dGCh'C
Cuts at: 0 115 215 532 589 682 765
Size: 115 100 317 57 93 83
Fragments arranged by size:
317 115 100 93 83 57
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 44 429 639 765
Size: 44 385 210 126
Fragments arranged by size:
385 210 126 44
BsrDI GCAATG_nn'
Cuts at: 0 27 765
Size: 27 738
BsrFI r'CCGG_y
Cuts at: 0 183 765
Size: 183 582
BssSI C'ACGA_G
Cuts at: 0 214 765
Size: 214 551
BstYI r'GATC_y
Cuts at: 0 665 765
Size: 665 100
Bsu36I CC'TnA_GG
Cuts at: 0 380 765
Size: 380 385
BtgI C'CryG_G
Cuts at: 0 195 566 765
Size: 195 371 199
Fragments arranged by size:
371 199 195
EaeI y'GGCC_r
Cuts at: 0 362 641 765
Size: 362 279 124
Fragments arranged by size:
362 279 124
EarI CTCTTCn'nnn_
Cuts at: 0 127 765
Size: 127 638
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 765
Size: 617 148
EcoO109I rG'GnC_Cy
Cuts at: 0 528 529 585 586 765
Size: 528 1 56 1 179
Fragments arranged by size:
528 179 56 1 1
EcoRV GAT'ATC
Cuts at: 0 573 765
Size: 573 192
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 684 765
Size: 684 81
MscI TGG'CCA
Cuts at: 0 364 643 765
Size: 364 279 122
Fragments arranged by size:
364 279 122
MspA1I CmG'CkG
Cuts at: 0 333 765
Size: 333 432
NaeI GCC'GGC
Cuts at: 0 185 765
Size: 185 580
NgoMIV G'CCGG_C
Cuts at: 0 183 765
Size: 183 582
NsiI A_TGCA'T
Cuts at: 0 402 502 765
Size: 402 100 263
Fragments arranged by size:
402 263 100
PflMI CCAn_nnn'nTGG
Cuts at: 0 201 650 765
Size: 201 449 115
Fragments arranged by size:
449 201 115
PfoI T'CCnGG_A
Cuts at: 0 425 765
Size: 425 340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 765
Size: 373 32 360
Fragments arranged by size:
373 360 32
PspOMI G'GGCC_C
Cuts at: 0 528 585 678 765
Size: 528 57 93 87
Fragments arranged by size:
528 93 87 57
PstI C_TGCA'G
Cuts at: 0 182 765
Size: 182 583
PvuII CAG'CTG
Cuts at: 0 333 765
Size: 333 432
SexAI A'CCwGG_T
Cuts at: 0 299 765
Size: 299 466
SfcI C'TryA_G
Cuts at: 0 178 765
Size: 178 587
StuI AGG'CCT
Cuts at: 0 293 765
Size: 293 472
StyI C'CwwG_G
Cuts at: 0 359 365 765
Size: 359 6 400
Fragments arranged by size:
400 359 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 37 135 493 765
Size: 37 98 358 272
Fragments arranged by size:
358 272 98 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 225 257 620 652 765
Size: 225 32 363 32 113
Fragments arranged by size:
363 225 113 32 32
Enzymes that do cut:
AarI ApaI ApoI AvaI BanI BanII BbsI BfrBI
Bme1580I BmrI BpmI BsaI BseRI BseYI BsgI BsmI
Bsp1286I BspMI BsrDI BsrFI BssSI BstYI Bsu36I BtgI
EaeI EarI Eco57MI EcoO109I EcoRV MmeI MscI MspA1I
NaeI NgoMIV NsiI PflMI PfoI PpiI PspOMI PstI
PvuII SexAI SfcI StuI StyI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NarI
NcoI NdeI NheI NotI NruI NspI PacI PasI
PciI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI