Restriction map for
pFN21AE6268
(Linear) (Six-base) MAPSORT of: KIEE6268.seq Check: 872 from: 1 to: 807
KIEE6268.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 178 562 678 807
Size: 178 384 116 129
Fragments arranged by size:
384 178 129 116
Acc65I G'GTAC_C
Cuts at: 0 161 807
Size: 161 646
AleI CACnn'nnGTG
Cuts at: 0 28 807
Size: 28 779
ApaLI G'TGCA_C
Cuts at: 0 319 691 807
Size: 319 372 116
Fragments arranged by size:
372 319 116
BanI G'GyrC_C
Cuts at: 0 161 436 807
Size: 161 275 371
Fragments arranged by size:
371 275 161
BanII G_rGCy'C
Cuts at: 0 42 807
Size: 42 765
BbsI GAAGACnn'nnnn_
Cuts at: 0 744 807
Size: 744 63
Bme1580I G_kGCm'C
Cuts at: 0 323 695 807
Size: 323 372 112
Fragments arranged by size:
372 323 112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 303 807
Size: 303 504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 216 543 612 807
Size: 56 160 327 69 195
Fragments arranged by size:
327 195 160 69 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 135 719 768 807
Size: 135 584 49 39
Fragments arranged by size:
584 135 49 39
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 319 331 349 361 376 406 730 760
Size: 319 12 18 12 15 30 324 30
Cuts at: 760 807
Size: 47
Fragments arranged by size:
324 319 47 30 30 18 15 12
12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 219 807
Size: 219 588
BseYI C'CCAG_C
Cuts at: 0 197 252 476 607 807
Size: 197 55 224 131 200
Fragments arranged by size:
224 200 197 131 55
BsiEI CG_ry'CG
Cuts at: 0 681 807
Size: 681 126
BsiHKAI G_wGCw'C
Cuts at: 0 323 695 807
Size: 323 372 112
Fragments arranged by size:
372 323 112
BsmBI CGTCTCn'nnnn_
Cuts at: 0 396 807
Size: 396 411
Bsp1286I G_dGCh'C
Cuts at: 0 42 323 695 807
Size: 42 281 372 112
Fragments arranged by size:
372 281 112 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 117 178 562 678 807
Size: 117 61 384 116 129
Fragments arranged by size:
384 129 117 116 61
BsrDI GCAATG_nn'
Cuts at: 0 362 807
Size: 362 445
BsrFI r'CCGG_y
Cuts at: 0 257 433 527 680 807
Size: 257 176 94 153 127
Fragments arranged by size:
257 176 153 127 94
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 807
Size: 30 777
BstYI r'GATC_y
Cuts at: 0 407 807
Size: 407 400
BtsI GCAGTG_nn'
Cuts at: 0 216 807
Size: 216 591
EaeI y'GGCC_r
Cuts at: 0 678 807
Size: 678 129
EagI C'GGCC_G
Cuts at: 0 678 807
Size: 678 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 216 543 612 807
Size: 56 160 327 69 195
Fragments arranged by size:
327 195 160 69 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 520 807
Size: 520 287
EcoO109I rG'GnC_Cy
Cuts at: 0 54 179 201 236 807
Size: 54 125 22 35 571
Fragments arranged by size:
571 125 54 35 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 319 351 807
Size: 319 32 456
Fragments arranged by size:
456 319 32
KpnI G_GTAC'C
Cuts at: 0 165 807
Size: 165 642
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 807
Size: 362 445
MslI CAynn'nnrTG
Cuts at: 0 28 807
Size: 28 779
MspA1I CmG'CkG
Cuts at: 0 18 116 197 414 632 807
Size: 18 98 81 217 218 175
Fragments arranged by size:
218 217 175 98 81 18
NaeI GCC'GGC
Cuts at: 0 259 435 807
Size: 259 176 372
Fragments arranged by size:
372 259 176
NgoMIV G'CCGG_C
Cuts at: 0 257 433 807
Size: 257 176 374
Fragments arranged by size:
374 257 176
PflMI CCAn_nnn'nTGG
Cuts at: 0 116 197 807
Size: 116 81 610
Fragments arranged by size:
610 116 81
PfoI T'CCnGG_A
Cuts at: 0 232 807
Size: 232 575
PpuMI rG'GwC_Cy
Cuts at: 0 54 179 201 236 807
Size: 54 125 22 35 571
Fragments arranged by size:
571 125 54 35 22
PstI C_TGCA'G
Cuts at: 0 140 491 521 807
Size: 140 351 30 286
Fragments arranged by size:
351 286 140 30
PvuII CAG'CTG
Cuts at: 0 116 197 414 632 807
Size: 116 81 217 218 175
Fragments arranged by size:
218 217 175 116 81
SanDI GG'GwC_CC
Cuts at: 0 54 179 807
Size: 54 125 628
Fragments arranged by size:
628 125 54
SfcI C'TryA_G
Cuts at: 0 136 487 517 807
Size: 136 351 30 290
Fragments arranged by size:
351 290 136 30
TatI w'GTAC_w
Cuts at: 0 140 807
Size: 140 667
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 730 762 807
Size: 730 32 45
Fragments arranged by size:
730 45 32
Enzymes that do cut:
AarI Acc65I AleI ApaLI BanI BanII BbsI Bme1580I
BplI BpmI BsaI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrDI BsrFI BstXI BstYI BtsI
EaeI EagI Eco57MI EcoNI EcoO109I Hin4I KpnI MmeI
MslI MspA1I NaeI NgoMIV PflMI PfoI PpuMI PstI
PvuII SanDI SfcI TatI TstI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsgI
BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI MfeI MluI MscI NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI