Restriction map for
pFN21AE6267
(Linear) (Six-base) MAPSORT of: KIEE6267.seq Check: 1246 from: 1 to: 903
KIEE6267.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 716 903
Size: 687 29 187
Fragments arranged by size:
687 187 29
AleI CACnn'nnGTG
Cuts at: 0 676 903
Size: 676 227
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 520 903
Size: 520 383
AlwNI CAG_nnn'CTG
Cuts at: 0 72 324 409 492 831 888 903
Size: 72 252 85 83 339 57 15
Fragments arranged by size:
339 252 85 83 72 57 15
ApaI G_GGCC'C
Cuts at: 0 237 661 814 903
Size: 237 424 153 89
Fragments arranged by size:
424 237 153 89
AvaI C'yCGr_G
Cuts at: 0 601 647 903
Size: 601 46 256
Fragments arranged by size:
601 256 46
BanI G'GyrC_C
Cuts at: 0 616 729 903
Size: 616 113 174
Fragments arranged by size:
616 174 113
BanII G_rGCy'C
Cuts at: 0 237 521 661 814 903
Size: 237 284 140 153 89
Fragments arranged by size:
284 237 153 140 89
BbsI GAAGACnn'nnnn_
Cuts at: 0 693 903
Size: 693 210
BbvCI CC'TCA_GC
Cuts at: 0 876 903
Size: 876 27
BciVI GTATCCnnnnn_n'
Cuts at: 0 38 903
Size: 38 865
Bme1580I G_kGCm'C
Cuts at: 0 237 499 661 814 903
Size: 237 262 162 153 89
Fragments arranged by size:
262 237 162 153 89
BmgBI CAC'GTC
Cuts at: 0 664 903
Size: 664 239
BmrI ACTGGGnnnn_n'
Cuts at: 0 509 903
Size: 509 394
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 444 903
Size: 444 459
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 903
Size: 586 317
Bpu10I CC'TnA_GC
Cuts at: 0 824 876 903
Size: 824 52 27
Fragments arranged by size:
824 52 27
BsaAI yAC'GTr
Cuts at: 0 213 678 903
Size: 213 465 225
Fragments arranged by size:
465 225 213
BsaBI GATnn'nnATC
Cuts at: 0 205 589 903
Size: 205 384 314
Fragments arranged by size:
384 314 205
BsaHI Gr'CG_yC
Cuts at: 0 279 903
Size: 279 624
BsaWI w'CCGG_w
Cuts at: 0 41 903
Size: 41 862
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 38 68 89 119 903
Size: 38 30 21 30 784
Fragments arranged by size:
784 38 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 903
Size: 97 806
BseYI C'CCAG_C
Cuts at: 0 686 903
Size: 686 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 781 903
Size: 337 444 122
Fragments arranged by size:
444 337 122
BsmBI CGTCTCn'nnnn_
Cuts at: 0 231 670 903
Size: 231 439 233
Fragments arranged by size:
439 233 231
Bsp1286I G_dGCh'C
Cuts at: 0 237 499 521 661 814 903
Size: 237 262 22 140 153 89
Fragments arranged by size:
262 237 153 140 89 22
BspEI T'CCGG_A
Cuts at: 0 41 903
Size: 41 862
BstXI CCAn_nnnn'nTGG
Cuts at: 0 177 213 903
Size: 177 36 690
Fragments arranged by size:
690 177 36
BstYI r'GATC_y
Cuts at: 0 828 903
Size: 828 75
Bsu36I CC'TnA_GG
Cuts at: 0 806 903
Size: 806 97
BtgI C'CryG_G
Cuts at: 0 611 903
Size: 611 292
BtsI GCAGTG_nn'
Cuts at: 0 73 903
Size: 73 830
EaeI y'GGCC_r
Cuts at: 0 191 260 524 903
Size: 191 69 264 379
Fragments arranged by size:
379 264 191 69
EarI CTCTTCn'nnn_
Cuts at: 0 673 903
Size: 673 230
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 687 716 903
Size: 586 101 29 187
Fragments arranged by size:
586 187 101 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 484 903
Size: 484 419
EcoO109I rG'GnC_Cy
Cuts at: 0 15 233 651 691 810 903
Size: 15 218 418 40 119 93
Fragments arranged by size:
418 218 119 93 40 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 820 852 903
Size: 820 32 51
Fragments arranged by size:
820 51 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 842 903
Size: 255 587 61
Fragments arranged by size:
587 255 61
MscI TGG'CCA
Cuts at: 0 262 526 903
Size: 262 264 377
Fragments arranged by size:
377 264 262
MslI CAynn'nnrTG
Cuts at: 0 211 313 502 676 903
Size: 211 102 189 174 227
Fragments arranged by size:
227 211 189 174 102
MspA1I CmG'CkG
Cuts at: 0 783 903
Size: 783 120
NcoI C'CATG_G
Cuts at: 0 611 903
Size: 611 292
PasI CC'CwG_GG
Cuts at: 0 287 903
Size: 287 616
PflMI CCAn_nnn'nTGG
Cuts at: 0 786 903
Size: 786 117
PfoI T'CCnGG_A
Cuts at: 0 479 530 903
Size: 479 51 373
Fragments arranged by size:
479 373 51
PpuMI rG'GwC_Cy
Cuts at: 0 651 691 903
Size: 651 40 212
Fragments arranged by size:
651 212 40
PspOMI G'GGCC_C
Cuts at: 0 233 657 810 903
Size: 233 424 153 93
Fragments arranged by size:
424 233 153 93
PvuII CAG'CTG
Cuts at: 0 783 903
Size: 783 120
SfcI C'TryA_G
Cuts at: 0 376 903
Size: 376 527
SmaI CCC'GGG
Cuts at: 0 649 903
Size: 649 254
StyI C'CwwG_G
Cuts at: 0 114 416 611 903
Size: 114 302 195 292
Fragments arranged by size:
302 292 195 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 76 127 205 604 717 903
Size: 76 51 78 399 113 186
Fragments arranged by size:
399 186 113 78 76 51
TatI w'GTAC_w
Cuts at: 0 96 903
Size: 96 807
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 309 903
Size: 309 594
XmaI C'CCGG_G
Cuts at: 0 647 903
Size: 647 256
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI AvaI BanI BanII
BbsI BbvCI BciVI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsmBI Bsp1286I BspEI BstXI BstYI Bsu36I BtgI
BtsI EaeI EarI Eco57MI EcoNI EcoO109I Hin4I MmeI
MscI MslI MspA1I NcoI PasI PflMI PfoI PpuMI
PspOMI PvuII SfcI SmaI StyI TaqII TatI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BclI BfrBI BglI
BglII BlpI BmtI BpuEI BsaI BsiEI BsiHKAI BsiWI
BsmI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmnI ZraI