Restriction map for
pFN21AE6266
(Linear) (Six-base) MAPSORT of: KIEE6266.seq Check: 291 from: 1 to: 762
KIEE6266.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AccI GT'mk_AC
Cuts at: 0 707 762
Size: 707 55
Acc65I G'GTAC_C
Cuts at: 0 38 762
Size: 38 724
AflIII A'CryG_T
Cuts at: 0 506 762
Size: 506 256
ApaI G_GGCC'C
Cuts at: 0 629 762
Size: 629 133
ApoI r'AATT_y
Cuts at: 0 724 762
Size: 724 38
AvaI C'yCGr_G
Cuts at: 0 168 762
Size: 168 594
BanI G'GyrC_C
Cuts at: 0 38 311 762
Size: 38 273 451
Fragments arranged by size:
451 273 38
BanII G_rGCy'C
Cuts at: 0 629 762
Size: 629 133
BglI GCCn_nnn'nGGC
Cuts at: 0 692 762
Size: 692 70
Bme1580I G_kGCm'C
Cuts at: 0 424 629 762
Size: 424 205 133
Fragments arranged by size:
424 205 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 762
Size: 277 485
BsaHI Gr'CG_yC
Cuts at: 0 173 762
Size: 173 589
BsaWI w'CCGG_w
Cuts at: 0 395 762
Size: 395 367
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 538 762
Size: 538 224
BseYI C'CCAG_C
Cuts at: 0 240 455 562 762
Size: 240 215 107 200
Fragments arranged by size:
240 215 200 107
BsiWI C'GTAC_G
Cuts at: 0 306 762
Size: 306 456
BsmI GAATG_Cn'
Cuts at: 0 485 762
Size: 485 277
BsmBI CGTCTCn'nnnn_
Cuts at: 0 75 762
Size: 75 687
Bsp1286I G_dGCh'C
Cuts at: 0 424 629 762
Size: 424 205 133
Fragments arranged by size:
424 205 133
BspEI T'CCGG_A
Cuts at: 0 395 762
Size: 395 367
BsrDI GCAATG_nn'
Cuts at: 0 407 762
Size: 407 355
BsrFI r'CCGG_y
Cuts at: 0 577 762
Size: 577 185
BstAPI GCAn_nnn'nTGC
Cuts at: 0 195 572 762
Size: 195 377 190
Fragments arranged by size:
377 195 190
BstXI CCAn_nnnn'nTGG
Cuts at: 0 240 762
Size: 240 522
BstYI r'GATC_y
Cuts at: 0 61 762
Size: 61 701
BtgI C'CryG_G
Cuts at: 0 428 762
Size: 428 334
BtsI GCAGTG_nn'
Cuts at: 0 196 424 502 762
Size: 196 228 78 260
Fragments arranged by size:
260 228 196 78
DraIII CAC_nnn'GTG
Cuts at: 0 734 762
Size: 734 28
EaeI y'GGCC_r
Cuts at: 0 2 684 762
Size: 2 682 78
Fragments arranged by size:
682 78 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 596 762
Size: 596 166
EcoO109I rG'GnC_Cy
Cuts at: 0 625 626 654 762
Size: 625 1 28 108
Fragments arranged by size:
625 108 28 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 53 85 320 352 762
Size: 53 32 235 32 410
Fragments arranged by size:
410 235 53 32 32
KpnI G_GTAC'C
Cuts at: 0 42 762
Size: 42 720
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 762
Size: 344 418
MscI TGG'CCA
Cuts at: 0 4 686 762
Size: 4 682 76
Fragments arranged by size:
682 76 4
MspA1I CmG'CkG
Cuts at: 0 430 552 762
Size: 430 122 210
Fragments arranged by size:
430 210 122
NaeI GCC'GGC
Cuts at: 0 579 762
Size: 579 183
NgoMIV G'CCGG_C
Cuts at: 0 577 762
Size: 577 185
NspI r_CATG'y
Cuts at: 0 570 762
Size: 570 192
PasI CC'CwG_GG
Cuts at: 0 20 762
Size: 20 742
PflMI CCAn_nnn'nTGG
Cuts at: 0 288 762
Size: 288 474
PspOMI G'GGCC_C
Cuts at: 0 625 762
Size: 625 137
PstI C_TGCA'G
Cuts at: 0 85 112 497 535 762
Size: 85 27 385 38 227
Fragments arranged by size:
385 227 85 38 27
SacII CC_GC'GG
Cuts at: 0 431 762
Size: 431 331
SfcI C'TryA_G
Cuts at: 0 81 108 493 531 762
Size: 81 27 385 38 231
Fragments arranged by size:
385 231 81 38 27
SgrAI Cr'CCGG_yG
Cuts at: 0 577 762
Size: 577 185
SmlI C'TyrA_G
Cuts at: 0 256 762
Size: 256 506
StyI C'CwwG_G
Cuts at: 0 48 762
Size: 48 714
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 183 329 762
Size: 183 146 433
Fragments arranged by size:
433 183 146
TatI w'GTAC_w
Cuts at: 0 320 762
Size: 320 442
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 200 232 762
Size: 200 32 530
Fragments arranged by size:
530 200 32
Enzymes that do cut:
AccI Acc65I AflIII ApaI ApoI AvaI BanI BanII
BglI Bme1580I BpuEI BsaHI BsaWI BseRI BseYI BsiWI
BsmI BsmBI Bsp1286I BspEI BsrDI BsrFI BstAPI BstXI
BstYI BtgI BtsI DraIII EaeI EciI EcoO109I Hin4I
KpnI MmeI MscI MspA1I NaeI NgoMIV NspI PasI
PflMI PspOMI PstI SacII SfcI SgrAI SmlI StyI
TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaXI BsgI BsiEI
BsiHKAI BspHI BspMI BsrBI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EagI EarI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
MfeI MluI MslI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI