Restriction map for
pFN21AE6265
(Linear) (Six-base) MAPSORT of: KIEE6265.seq Check: 8002 from: 1 to: 792
KIEE6265.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 132 516 595 604 738 792
Size: 132 384 79 9 134 54
Fragments arranged by size:
384 134 132 79 54 9
AccI GT'mk_AC
Cuts at: 0 739 792
Size: 739 53
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 394 470 792
Size: 72 322 76 322
Fragments arranged by size:
322 322 76 72
AflIII A'CryG_T
Cuts at: 0 460 792
Size: 460 332
AleI CACnn'nnGTG
Cuts at: 0 676 792
Size: 676 116
AlwNI CAG_nnn'CTG
Cuts at: 0 303 764 792
Size: 303 461 28
Fragments arranged by size:
461 303 28
AvaI C'yCGr_G
Cuts at: 0 314 743 792
Size: 314 429 49
Fragments arranged by size:
429 314 49
BanI G'GyrC_C
Cuts at: 0 616 703 792
Size: 616 87 89
Fragments arranged by size:
616 89 87
BanII G_rGCy'C
Cuts at: 0 130 293 577 792
Size: 130 163 284 215
Fragments arranged by size:
284 215 163 130
BglI GCCn_nnn'nGGC
Cuts at: 0 491 792
Size: 491 301
BlpI GC'TnA_GC
Cuts at: 0 10 792
Size: 10 782
Bme1580I G_kGCm'C
Cuts at: 0 706 792
Size: 706 86
BmgBI CAC'GTC
Cuts at: 0 363 792
Size: 363 429
BmrI ACTGGGnnnn_n'
Cuts at: 0 387 579 792
Size: 387 192 213
Fragments arranged by size:
387 213 192
Bpu10I CC'TnA_GC
Cuts at: 0 22 792
Size: 22 770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 792
Size: 561 231
BsaAI yAC'GTr
Cuts at: 0 461 792
Size: 461 331
BsaWI w'CCGG_w
Cuts at: 0 640 792
Size: 640 152
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 33 612 792
Size: 25 8 579 180
Fragments arranged by size:
579 180 25 8
BseYI C'CCAG_C
Cuts at: 0 476 792
Size: 476 316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 792
Size: 623 169
BsmI GAATG_Cn'
Cuts at: 0 59 120 792
Size: 59 61 672
Fragments arranged by size:
672 61 59
BsmBI CGTCTCn'nnnn_
Cuts at: 0 315 792
Size: 315 477
Bsp1286I G_dGCh'C
Cuts at: 0 130 293 577 706 792
Size: 130 163 284 129 86
Fragments arranged by size:
284 163 130 129 86
BspEI T'CCGG_A
Cuts at: 0 640 792
Size: 640 152
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 132 288 516 595 604 738 792
Size: 132 156 228 79 9 134 54
Fragments arranged by size:
228 156 134 132 79 54 9
BsrDI GCAATG_nn'
Cuts at: 0 234 507 723 792
Size: 234 273 216 69
Fragments arranged by size:
273 234 216 69
BsrFI r'CCGG_y
Cuts at: 0 75 472 560 792
Size: 75 397 88 232
Fragments arranged by size:
397 232 88 75
BssHII G'CGCG_C
Cuts at: 0 723 792
Size: 723 69
BssSI C'ACGA_G
Cuts at: 0 624 792
Size: 624 168
BstZ17I GTA'TAC
Cuts at: 0 740 792
Size: 740 52
BtsI GCAGTG_nn'
Cuts at: 0 125 212 222 792
Size: 125 87 10 570
Fragments arranged by size:
570 125 87 10
EaeI y'GGCC_r
Cuts at: 0 240 792
Size: 240 552
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 394 470 792
Size: 72 322 76 322
Fragments arranged by size:
322 322 76 72
EcoO109I rG'GnC_Cy
Cuts at: 0 646 792
Size: 646 146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 262 294 792
Size: 262 32 498
Fragments arranged by size:
498 262 32
HincII GTy'rAC
Cuts at: 0 105 792
Size: 105 687
MslI CAynn'nnrTG
Cuts at: 0 227 676 792
Size: 227 449 116
Fragments arranged by size:
449 227 116
MspA1I CmG'CkG
Cuts at: 0 164 792
Size: 164 628
NaeI GCC'GGC
Cuts at: 0 562 792
Size: 562 230
NgoMIV G'CCGG_C
Cuts at: 0 560 792
Size: 560 232
PflMI CCAn_nnn'nTGG
Cuts at: 0 476 792
Size: 476 316
PmlI CAC'GTG
Cuts at: 0 461 792
Size: 461 331
PspXI vC'TCGA_Gb
Cuts at: 0 743 792
Size: 743 49
PstI C_TGCA'G
Cuts at: 0 155 524 792
Size: 155 369 268
Fragments arranged by size:
369 268 155
SbfI CC_TGCA'GG
Cuts at: 0 155 792
Size: 155 637
ScaI AGT'ACT
Cuts at: 0 568 792
Size: 568 224
SexAI A'CCwGG_T
Cuts at: 0 773 792
Size: 773 19
SfcI C'TryA_G
Cuts at: 0 151 520 792
Size: 151 369 272
Fragments arranged by size:
369 272 151
SmlI C'TyrA_G
Cuts at: 0 576 743 792
Size: 576 167 49
Fragments arranged by size:
576 167 49
StuI AGG'CCT
Cuts at: 0 455 792
Size: 455 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 195 287 666 792
Size: 195 92 379 126
Fragments arranged by size:
379 195 126 92
TatI w'GTAC_w
Cuts at: 0 395 566 792
Size: 395 171 226
Fragments arranged by size:
395 226 171
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 178 792
Size: 178 614
XhoI C'TCGA_G
Cuts at: 0 743 792
Size: 743 49
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlwNI AvaI BanI
BanII BglI BlpI Bme1580I BmgBI BmrI Bpu10I BpuEI
BsaAI BsaWI BseRI BseYI BsgI BsmI BsmBI Bsp1286I
BspEI BspMI BsrDI BsrFI BssHII BssSI BstZ17I BtsI
EaeI Eco57MI EcoO109I Hin4I HincII MslI MspA1I NaeI
NgoMIV PflMI PmlI PspXI PstI SbfI ScaI SexAI
SfcI SmlI StuI TaqII TatI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmtI BplI BpmI BsaI BsaBI BsaHI
BsaXI BsiEI BsiHKAI BsiWI BspHI BsrBI BsrGI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StyI SwaI TstI
Tth111I XbaI XmaI XmnI ZraI