(Linear) (Six-base) MAPSORT of: KIEE6263.seq  Check: 9240  from: 1  to: 564



KIEE6263.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     335     359     564 
   Size:        335      24     205

  Fragments arranged by size: 

                 335     205      24
 
AfeI AGC'GCT

Cuts at:      0     427     564 
   Size:        427     137
 
AleI CACnn'nnGTG

Cuts at:      0     251     564 
   Size:        251     313
 
ApoI r'AATT_y

Cuts at:      0      11     478     564 
   Size:         11     467      86

  Fragments arranged by size: 

                 467      86      11
 
AvrII C'CTAG_G

Cuts at:      0     529     564 
   Size:        529      35
 
BanII G_rGCy'C

Cuts at:      0     281     368     564 
   Size:        281      87     196

  Fragments arranged by size: 

                 281     196      87
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     201     364     564 
   Size:        201     163     200

  Fragments arranged by size: 

                 201     200     163
 
BglII A'GATC_T

Cuts at:      0     327     564 
   Size:        327     237
 
Bme1580I G_kGCm'C

Cuts at:      0      70     290     564 
   Size:         70     220     274

  Fragments arranged by size: 

                 274     220      70
 
Bpu10I CC'TnA_GC

Cuts at:      0      71     564 
   Size:         71     493
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     187     424     564 
   Size:        187     237     140

  Fragments arranged by size: 

                 237     187     140
 
BsiHKAI G_wGCw'C

Cuts at:      0      77     325     368     564 
   Size:         77     248      43     196

  Fragments arranged by size: 

                 248     196      77      43
 
Bsp1286I G_dGCh'C

Cuts at:      0      70      77     281     290     325     368     564 
   Size:         70       7     204       9      35      43     196

  Fragments arranged by size: 

                 204     196      70      43      35       9       7
 
BstEII G'GTnAC_C

Cuts at:      0     418     564 
   Size:        418     146
 
BstYI r'GATC_y

Cuts at:      0     299     327     564 
   Size:        299      28     237

  Fragments arranged by size: 

                 299     237      28
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      30     564 
   Size:         30     534
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     205     564 
   Size:        205     359
 
EcoICRI GAG'CTC

Cuts at:      0     366     564 
   Size:        366     198
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     335     359     564 
   Size:        335      24     205

  Fragments arranged by size: 

                 335     205      24
 
EcoRI G'AATT_C

Cuts at:      0     478     564 
   Size:        478      86
 
HaeII r_GCGC'y

Cuts at:      0     429     564 
   Size:        429     135
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     312     344     564 
   Size:        312      32     220

  Fragments arranged by size: 

                 312     220      32
 
HincII GTy'rAC

Cuts at:      0      82     564 
   Size:         82     482
 
MslI CAynn'nnrTG

Cuts at:      0     251     265     564 
   Size:        251      14     299

  Fragments arranged by size: 

                 299     251      14
 
SacI G_AGCT'C

Cuts at:      0     368     564 
   Size:        368     196
 
StuI AGG'CCT

Cuts at:      0     544     564 
   Size:        544      20
 
StyI C'CwwG_G

Cuts at:      0     167     529     564 
   Size:        167     362      35

  Fragments arranged by size: 

                 362     167      35
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     174     564 
   Size:        174     390
 
XmnI GAAnn'nnTTC

Cuts at:      0     112     564 
   Size:        112     452
 

 Enzymes that do cut:

     AcuI     AfeI     AleI     ApoI    AvrII    BanII     BbsI    BglII
 Bme1580I   Bpu10I    BseRI  BsiHKAI Bsp1286I   BstEII    BstYI    CspCI
     DrdI  EcoICRI  Eco57MI    EcoRI    HaeII    Hin4I   HincII     MslI
     SacI     StuI     StyI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI     DraI   DraIII     EaeI     EagI     EarI     EciI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI