(Linear) (Six-base) MAPSORT of: KIEE6260.seq  Check: 6044  from: 1  to: 552



KIEE6260.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     208     552 
   Size:        208     344
 
AfeI AGC'GCT

Cuts at:      0     226     552 
   Size:        226     326
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     199     226     552 
   Size:        199      27     326

  Fragments arranged by size: 

                 326     199      27
 
ApaLI G'TGCA_C

Cuts at:      0     327     552 
   Size:        327     225
 
AvaI C'yCGr_G

Cuts at:      0      10     431     470     552 
   Size:         10     421      39      82

  Fragments arranged by size: 

                 421      82      39      10
 
BglI GCCn_nnn'nGGC

Cuts at:      0     473     552 
   Size:        473      79
 
Bme1580I G_kGCm'C

Cuts at:      0     331     552 
   Size:        331     221
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     142     540     552 
   Size:        142     398      12

  Fragments arranged by size: 

                 398     142      12
 
BsaAI yAC'GTr

Cuts at:      0     323     552 
   Size:        323     229
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     157     160     552 
   Size:        157       3     392

  Fragments arranged by size: 

                 392     157       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     552 
   Size:        249     303
 
BsiHKAI G_wGCw'C

Cuts at:      0     331     552 
   Size:        331     221
 
Bsp1286I G_dGCh'C

Cuts at:      0     331     552 
   Size:        331     221
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     208     271     394     552 
   Size:        208      63     123     158

  Fragments arranged by size: 

                 208     158     123      63
 
BsrDI GCAATG_nn'

Cuts at:      0     275     414     539     552 
   Size:        275     139     125      13

  Fragments arranged by size: 

                 275     139     125      13
 
BsrFI r'CCGG_y

Cuts at:      0     520     552 
   Size:        520      32
 
BstEII G'GTnAC_C

Cuts at:      0     375     552 
   Size:        375     177
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     360     552 
   Size:        360     192
 
BtgI C'CryG_G

Cuts at:      0      96     282     552 
   Size:         96     186     270

  Fragments arranged by size: 

                 270     186      96
 
DraIII CAC_nnn'GTG

Cuts at:      0     326     552 
   Size:        326     226
 
EaeI y'GGCC_r

Cuts at:      0      46     255     552 
   Size:         46     209     297

  Fragments arranged by size: 

                 297     209      46
 
EarI CTCTTCn'nnn_

Cuts at:      0      71      77     134     552 
   Size:         71       6      57     418

  Fragments arranged by size: 

                 418      71      57       6
 
EcoO109I rG'GnC_Cy

Cuts at:      0     368     441     552 
   Size:        368      73     111

  Fragments arranged by size: 

                 368     111      73
 
HaeII r_GCGC'y

Cuts at:      0      10     228     552 
   Size:         10     218     324

  Fragments arranged by size: 

                 324     218      10
 
MscI TGG'CCA

Cuts at:      0     257     552 
   Size:        257     295
 
MspA1I CmG'CkG

Cuts at:      0     338     552 
   Size:        338     214
 
NaeI GCC'GGC

Cuts at:      0     522     552 
   Size:        522      30
 
NgoMIV G'CCGG_C

Cuts at:      0     520     552 
   Size:        520      32
 
PasI CC'CwG_GG

Cuts at:      0     215     552 
   Size:        215     337
 
PfoI T'CCnGG_A

Cuts at:      0     437     552 
   Size:        437     115
 
PmlI CAC'GTG

Cuts at:      0     323     552 
   Size:        323     229
 
PpuMI rG'GwC_Cy

Cuts at:      0     368     441     552 
   Size:        368      73     111

  Fragments arranged by size: 

                 368     111      73
 
PvuII CAG'CTG

Cuts at:      0     338     552 
   Size:        338     214
 
SanDI GG'GwC_CC

Cuts at:      0     368     552 
   Size:        368     184
 
SmaI CCC'GGG

Cuts at:      0     472     552 
   Size:        472      80
 
StuI AGG'CCT

Cuts at:      0     302     552 
   Size:        302     250
 
StyI C'CwwG_G

Cuts at:      0     525     552 
   Size:        525      27
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     328     552 
   Size:        328     224
 
XmaI C'CCGG_G

Cuts at:      0     470     552 
   Size:        470      82
 

 Enzymes that do cut:

     AarI     AfeI     AlfI    ApaLI     AvaI     BglI Bme1580I     BsaI
    BsaAI    BseRI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI    BsrFI
   BstEII    BstXI     BtgI   DraIII     EaeI     EarI EcoO109I    HaeII
     MscI   MspA1I     NaeI   NgoMIV     PasI     PfoI     PmlI    PpuMI
    PvuII    SanDI     SmaI     StuI     StyI    TaqII     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI