Restriction map for
pFN21AE6259
(Linear) (Six-base) MAPSORT of: KIEE6259.seq Check: 5712 from: 1 to: 723
KIEE6259.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AccI GT'mk_AC
Cuts at: 0 591 723
Size: 591 132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 698 723
Size: 631 67 25
Fragments arranged by size:
631 67 25
AlwNI CAG_nnn'CTG
Cuts at: 0 553 723
Size: 553 170
BanI G'GyrC_C
Cuts at: 0 197 723
Size: 197 526
BanII G_rGCy'C
Cuts at: 0 17 160 529 723
Size: 17 143 369 194
Fragments arranged by size:
369 194 143 17
BbeI G_GCGC'C
Cuts at: 0 201 723
Size: 201 522
BbsI GAAGACnn'nnnn_
Cuts at: 0 661 710 723
Size: 661 49 13
Fragments arranged by size:
661 49 13
BclI T'GATC_A
Cuts at: 0 515 644 723
Size: 515 129 79
Fragments arranged by size:
515 129 79
BmrI ACTGGGnnnn_n'
Cuts at: 0 532 723
Size: 532 191
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 708 723
Size: 708 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 409 723
Size: 130 279 314
Fragments arranged by size:
314 279 130
BsaI GGTCTCn'nnnn_
Cuts at: 0 34 723
Size: 34 689
BsaHI Gr'CG_yC
Cuts at: 0 61 198 723
Size: 61 137 525
Fragments arranged by size:
525 137 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 46 76 100 130 584 614 723
Size: 46 30 24 30 454 30 109
Fragments arranged by size:
454 109 46 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 322 723
Size: 7 315 401
Fragments arranged by size:
401 315 7
BseYI C'CCAG_C
Cuts at: 0 153 546 723
Size: 153 393 177
Fragments arranged by size:
393 177 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 723
Size: 118 605
BsiEI CG_ry'CG
Cuts at: 0 33 723
Size: 33 690
BsiHKAI G_wGCw'C
Cuts at: 0 509 723
Size: 509 214
Bsp1286I G_dGCh'C
Cuts at: 0 17 160 509 529 723
Size: 17 143 349 20 194
Fragments arranged by size:
349 194 143 20 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 723
Size: 134 589
BsrFI r'CCGG_y
Cuts at: 0 638 723
Size: 638 85
BstAPI GCAn_nnn'nTGC
Cuts at: 0 96 723
Size: 96 627
BstXI CCAn_nnnn'nTGG
Cuts at: 0 343 723
Size: 343 380
BtsI GCAGTG_nn'
Cuts at: 0 64 723
Size: 64 659
DraIII CAC_nnn'GTG
Cuts at: 0 513 723
Size: 513 210
EaeI y'GGCC_r
Cuts at: 0 30 723
Size: 30 693
EagI C'GGCC_G
Cuts at: 0 30 723
Size: 30 693
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 108 523 723
Size: 108 415 200
Fragments arranged by size:
415 200 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 698 723
Size: 631 67 25
Fragments arranged by size:
631 67 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 22 723
Size: 22 701
HaeII r_GCGC'y
Cuts at: 0 81 201 723
Size: 81 120 522
Fragments arranged by size:
522 120 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 407 439 723
Size: 46 32 329 32 284
Fragments arranged by size:
329 284 46 32 32
HincII GTy'rAC
Cuts at: 0 369 723
Size: 369 354
HindIII A'AGCT_T
Cuts at: 0 463 723
Size: 463 260
KasI G'GCGC_C
Cuts at: 0 197 723
Size: 197 526
MslI CAynn'nnrTG
Cuts at: 0 449 723
Size: 449 274
MspA1I CmG'CkG
Cuts at: 0 553 723
Size: 553 170
NarI GG'CG_CC
Cuts at: 0 198 723
Size: 198 525
NotI GC'GGCC_GC
Cuts at: 0 30 723
Size: 30 693
NspI r_CATG'y
Cuts at: 0 51 174 436 723
Size: 51 123 262 287
Fragments arranged by size:
287 262 123 51
PstI C_TGCA'G
Cuts at: 0 194 723
Size: 194 529
PvuII CAG'CTG
Cuts at: 0 553 723
Size: 553 170
SfcI C'TryA_G
Cuts at: 0 190 592 723
Size: 190 402 131
Fragments arranged by size:
402 190 131
SfoI GGC'GCC
Cuts at: 0 199 723
Size: 199 524
SmlI C'TyrA_G
Cuts at: 0 145 424 723
Size: 145 279 299
Fragments arranged by size:
299 279 145
SspI AAT'ATT
Cuts at: 0 186 723
Size: 186 537
StyI C'CwwG_G
Cuts at: 0 164 247 362 585 723
Size: 164 83 115 223 138
Fragments arranged by size:
223 164 138 115 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 58 723
Size: 58 665
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 100 132 338 370 723
Size: 100 32 206 32 353
Fragments arranged by size:
353 206 100 32 32
Enzymes that do cut:
AccI AcuI AlwNI BanI BanII BbeI BbsI BclI
BmrI BplI BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstXI
BtsI DraIII EaeI EagI EciI Eco57MI EcoNI HaeII
Hin4I HincII HindIII KasI MslI MspA1I NarI NotI
NspI PstI PvuII SfcI SfoI SmlI SspI StyI
TaqII TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI
BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI
BpmI Bpu10I BsaAI BsaBI BsaWI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DrdI EarI EcoICRI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HpaI KpnI MfeI MluI MmeI
MscI NaeI NcoI NdeI NgoMIV NheI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI