Restriction map for
pFN21AE6257
(Linear) (Six-base) MAPSORT of: KIEE6257.seq Check: 4898 from: 1 to: 741
KIEE6257.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 741
Size: 461 280
AflII C'TTAA_G
Cuts at: 0 280 741
Size: 280 461
AgeI A'CCGG_T
Cuts at: 0 182 741
Size: 182 559
AlwNI CAG_nnn'CTG
Cuts at: 0 661 741
Size: 661 80
BamHI G'GATC_C
Cuts at: 0 443 741
Size: 443 298
BanII G_rGCy'C
Cuts at: 0 677 741
Size: 677 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 384 741
Size: 384 357
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 15 49 741
Size: 15 34 692
Fragments arranged by size:
692 34 15
BclI T'GATC_A
Cuts at: 0 65 741
Size: 65 676
BmrI ACTGGGnnnn_n'
Cuts at: 0 570 741
Size: 570 171
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 721 741
Size: 721 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 716 741
Size: 184 532 25
Fragments arranged by size:
532 184 25
BsaI GGTCTCn'nnnn_
Cuts at: 0 135 370 741
Size: 135 235 371
Fragments arranged by size:
371 235 135
BsaWI w'CCGG_w
Cuts at: 0 182 741
Size: 182 559
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 564 741
Size: 564 177
BseYI C'CCAG_C
Cuts at: 0 76 741
Size: 76 665
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 9 741
Size: 9 732
BsiEI CG_ry'CG
Cuts at: 0 183 741
Size: 183 558
BsmBI CGTCTCn'nnnn_
Cuts at: 0 546 741
Size: 546 195
Bsp1286I G_dGCh'C
Cuts at: 0 677 741
Size: 677 64
BsrFI r'CCGG_y
Cuts at: 0 182 741
Size: 182 559
BstYI r'GATC_y
Cuts at: 0 443 741
Size: 443 298
BtgI C'CryG_G
Cuts at: 0 447 668 741
Size: 447 221 73
Fragments arranged by size:
447 221 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 290 741
Size: 290 451
DrdI GACnn_nn'nnGTC
Cuts at: 0 183 741
Size: 183 558
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 331 741
Size: 331 410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 461 716 741
Size: 184 277 255 25
Fragments arranged by size:
277 255 184 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 244 546 741
Size: 244 302 195
Fragments arranged by size:
302 244 195
EcoO109I rG'GnC_Cy
Cuts at: 0 81 701 741
Size: 81 620 40
Fragments arranged by size:
620 81 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 317 342 349 741
Size: 310 7 25 7 392
Fragments arranged by size:
392 310 25 7 7
MspA1I CmG'CkG
Cuts at: 0 694 741
Size: 694 47
NcoI C'CATG_G
Cuts at: 0 447 668 741
Size: 447 221 73
Fragments arranged by size:
447 221 73
PpuMI rG'GwC_Cy
Cuts at: 0 81 701 741
Size: 81 620 40
Fragments arranged by size:
620 81 40
PvuII CAG'CTG
Cuts at: 0 694 741
Size: 694 47
SanDI GG'GwC_CC
Cuts at: 0 81 701 741
Size: 81 620 40
Fragments arranged by size:
620 81 40
SfcI C'TryA_G
Cuts at: 0 244 741
Size: 244 497
SmlI C'TyrA_G
Cuts at: 0 280 741
Size: 280 461
SspI AAT'ATT
Cuts at: 0 408 741
Size: 408 333
StyI C'CwwG_G
Cuts at: 0 447 668 741
Size: 447 221 73
Fragments arranged by size:
447 221 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 193 345 741
Size: 193 152 396
Fragments arranged by size:
396 193 152
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 345 741
Size: 345 396
Enzymes that do cut:
AcuI AflII AgeI AlwNI BamHI BanII BbsI BcgI
BclI BmrI BplI BpmI BsaI BsaWI BseRI BseYI
BsgI BsiEI BsmBI Bsp1286I BsrFI BstYI BtgI BtgZI
DrdI EciI Eco57MI EcoNI EcoO109I Hin4I MspA1I NcoI
PpuMI PvuII SanDI SfcI SmlI SspI StyI TaqII
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaXI BsiHKAI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII EaeI EagI EarI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI SwaI TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI