Restriction map for
pFN21AE6254
(Linear) (Six-base) MAPSORT of: KIEE6254.seq Check: 7135 from: 1 to: 885
KIEE6254.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 395 509 651 885
Size: 395 114 142 234
Fragments arranged by size:
395 234 142 114
AflIII A'CryG_T
Cuts at: 0 14 885
Size: 14 871
AleI CACnn'nnGTG
Cuts at: 0 436 885
Size: 436 449
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 364 454 885
Size: 364 90 431
Fragments arranged by size:
431 364 90
AlwNI CAG_nnn'CTG
Cuts at: 0 285 321 885
Size: 285 36 564
Fragments arranged by size:
564 285 36
ApaI G_GGCC'C
Cuts at: 0 149 533 578 679 885
Size: 149 384 45 101 206
Fragments arranged by size:
384 206 149 101 45
AvaI C'yCGr_G
Cuts at: 0 113 142 633 782 885
Size: 113 29 491 149 103
Fragments arranged by size:
491 149 113 103 29
BanI G'GyrC_C
Cuts at: 0 94 885
Size: 94 791
BanII G_rGCy'C
Cuts at: 0 143 149 374 533 578 679 885
Size: 143 6 225 159 45 101 206
Fragments arranged by size:
225 206 159 143 101 45 6
BbeI G_GCGC'C
Cuts at: 0 98 885
Size: 98 787
BbsI GAAGACnn'nnnn_
Cuts at: 0 99 885
Size: 99 786
BglI GCCn_nnn'nGGC
Cuts at: 0 67 76 118 568 885
Size: 67 9 42 450 317
Fragments arranged by size:
450 317 67 42 9
Bme1580I G_kGCm'C
Cuts at: 0 149 443 533 578 679 885
Size: 149 294 90 45 101 206
Fragments arranged by size:
294 206 149 101 90 45
BmgBI CAC'GTC
Cuts at: 0 584 885
Size: 584 301
BmrI ACTGGGnnnn_n'
Cuts at: 0 82 885
Size: 82 803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 885
Size: 670 215
BsaAI yAC'GTr
Cuts at: 0 657 885
Size: 657 228
BsaHI Gr'CG_yC
Cuts at: 0 95 885
Size: 95 790
BseYI C'CCAG_C
Cuts at: 0 154 321 359 672 885
Size: 154 167 38 313 213
Fragments arranged by size:
313 213 167 154 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 367 481 679 694 885
Size: 324 43 114 198 15 191
Fragments arranged by size:
324 198 191 114 43 15
BsiEI CG_ry'CG
Cuts at: 0 572 885
Size: 572 313
BsiHKAI G_wGCw'C
Cuts at: 0 143 374 780 885
Size: 143 231 406 105
Fragments arranged by size:
406 231 143 105
Bsp1286I G_dGCh'C
Cuts at: 0 143 149 374 443 533 578 679 780
Size: 143 6 225 69 90 45 101 101
Cuts at: 780 885
Size: 105
Fragments arranged by size:
225 143 105 101 101 90 69 45
6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 182 389 395 509 651 885
Size: 182 207 6 114 142 234
Fragments arranged by size:
234 207 182 142 114 6
BsrFI r'CCGG_y
Cuts at: 0 792 885
Size: 792 93
BstAPI GCAn_nnn'nTGC
Cuts at: 0 285 390 885
Size: 285 105 495
Fragments arranged by size:
495 285 105
BstEII G'GTnAC_C
Cuts at: 0 216 549 885
Size: 216 333 336
Fragments arranged by size:
336 333 216
Bsu36I CC'TnA_GG
Cuts at: 0 804 885
Size: 804 81
BtgI C'CryG_G
Cuts at: 0 444 885
Size: 444 441
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 136 885
Size: 136 749
BtsI GCAGTG_nn'
Cuts at: 0 864 885
Size: 864 21
EaeI y'GGCC_r
Cuts at: 0 59 236 272 569 608 756 885
Size: 59 177 36 297 39 148 129
Fragments arranged by size:
297 177 148 129 59 39 36
EagI C'GGCC_G
Cuts at: 0 569 885
Size: 569 316
EarI CTCTTCn'nnn_
Cuts at: 0 196 205 885
Size: 196 9 680
Fragments arranged by size:
680 196 9
EcoICRI GAG'CTC
Cuts at: 0 141 372 885
Size: 141 231 513
Fragments arranged by size:
513 231 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 885
Size: 670 215
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 683 885
Size: 52 631 202
Fragments arranged by size:
631 202 52
EcoO109I rG'GnC_Cy
Cuts at: 0 78 146 230 575 676 885
Size: 78 68 84 345 101 209
Fragments arranged by size:
345 209 101 84 78 68
FspI TGC'GCA
Cuts at: 0 430 885
Size: 430 455
HaeII r_GCGC'y
Cuts at: 0 98 454 885
Size: 98 356 431
Fragments arranged by size:
431 356 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 217 249 885
Size: 217 32 636
Fragments arranged by size:
636 217 32
KasI G'GCGC_C
Cuts at: 0 94 885
Size: 94 791
MslI CAynn'nnrTG
Cuts at: 0 346 436 885
Size: 346 90 449
Fragments arranged by size:
449 346 90
MspA1I CmG'CkG
Cuts at: 0 527 535 885
Size: 527 8 350
Fragments arranged by size:
527 350 8
NaeI GCC'GGC
Cuts at: 0 794 885
Size: 794 91
NarI GG'CG_CC
Cuts at: 0 95 885
Size: 95 790
NgoMIV G'CCGG_C
Cuts at: 0 792 885
Size: 792 93
NspI r_CATG'y
Cuts at: 0 330 885
Size: 330 555
PasI CC'CwG_GG
Cuts at: 0 149 885
Size: 149 736
PfoI T'CCnGG_A
Cuts at: 0 842 885
Size: 842 43
PshAI GACnn'nnGTC
Cuts at: 0 133 885
Size: 133 752
PspOMI G'GGCC_C
Cuts at: 0 145 529 574 675 885
Size: 145 384 45 101 210
Fragments arranged by size:
384 210 145 101 45
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 736 768 885
Size: 736 32 117
Fragments arranged by size:
736 117 32
PstI C_TGCA'G
Cuts at: 0 260 395 418 885
Size: 260 135 23 467
Fragments arranged by size:
467 260 135 23
SacI G_AGCT'C
Cuts at: 0 143 374 885
Size: 143 231 511
Fragments arranged by size:
511 231 143
SapI GCTCTTCn'nnn_
Cuts at: 0 196 205 885
Size: 196 9 680
Fragments arranged by size:
680 196 9
SbfI CC_TGCA'GG
Cuts at: 0 260 885
Size: 260 625
SfcI C'TryA_G
Cuts at: 0 256 391 414 522 885
Size: 256 135 23 108 363
Fragments arranged by size:
363 256 135 108 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 67 76 568 885
Size: 67 9 492 317
Fragments arranged by size:
492 317 67 9
SfoI GGC'GCC
Cuts at: 0 96 885
Size: 96 789
SmaI CCC'GGG
Cuts at: 0 115 885
Size: 115 770
SphI G_CATG'C
Cuts at: 0 330 885
Size: 330 555
StyI C'CwwG_G
Cuts at: 0 74 221 885
Size: 74 147 664
Fragments arranged by size:
664 147 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 829 885
Size: 829 56
TatI w'GTAC_w
Cuts at: 0 665 885
Size: 665 220
XmaI C'CCGG_G
Cuts at: 0 113 885
Size: 113 772
XmnI GAAnn'nnTTC
Cuts at: 0 748 885
Size: 748 137
Enzymes that do cut:
AarI AflIII AleI AlfI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BglI Bme1580I BmgBI BmrI BpmI
BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrFI BstAPI BstEII Bsu36I BtgI BtgZI BtsI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I KasI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PfoI PshAI PspOMI PsrI PstI SacI SapI
SbfI SfcI SfiI SfoI SmaI SphI StyI TaqII
TatI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I
ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NcoI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PpuMI
PsiI PspXI PvuI PvuII RsrII SacII SalI SanDI
ScaI SexAI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI
ZraI