(Linear) (Six-base) MAPSORT of: KIEE6252.seq  Check: 1443  from: 1  to: 663



KIEE6252.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AatII G_ACGT'C

Cuts at:      0      87     663 
   Size:         87     576
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     557     663 
   Size:        557     106
 
AlwNI CAG_nnn'CTG

Cuts at:      0      75     123     489     663 
   Size:         75      48     366     174

  Fragments arranged by size: 

                 366     174      75      48
 
ApoI r'AATT_y

Cuts at:      0     244     663 
   Size:        244     419
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     460     493     663 
   Size:        460      33     170

  Fragments arranged by size: 

                 460     170      33
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      67     101     663 
   Size:         67      34     562

  Fragments arranged by size: 

                 562      67      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     461     663 
   Size:        461     202
 
BmgBI CAC'GTC

Cuts at:      0      27     663 
   Size:         27     636
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     295     663 
   Size:        295     368
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     510     511     663 
   Size:        163     347       1     152

  Fragments arranged by size: 

                 347     163     152       1
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     663 
   Size:        118     545
 
BsaHI Gr'CG_yC

Cuts at:      0      84     663 
   Size:         84     579
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     155     663 
   Size:        155     508
 
BsiEI CG_ry'CG

Cuts at:      0      52     663 
   Size:         52     611
 
BsmI GAATG_Cn'

Cuts at:      0     361     663 
   Size:        361     302
 
BsrDI GCAATG_nn'

Cuts at:      0     234     663 
   Size:        234     429
 
BssHII G'CGCG_C

Cuts at:      0      41     663 
   Size:         41     622
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      75     123     663 
   Size:         75      48     540

  Fragments arranged by size: 

                 540      75      48
 
BstEII G'GTnAC_C

Cuts at:      0     334     663 
   Size:        334     329
 
BstYI r'GATC_y

Cuts at:      0     414     663 
   Size:        414     249
 
BtsI GCAGTG_nn'

Cuts at:      0     316     663 
   Size:        316     347
 
EaeI y'GGCC_r

Cuts at:      0      49     663 
   Size:         49     614
 
EagI C'GGCC_G

Cuts at:      0      49     663 
   Size:         49     614
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     510     511     557     663 
   Size:        163     347       1      46     106

  Fragments arranged by size: 

                 347     163     106      46       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     487     519     663 
   Size:        487      32     144

  Fragments arranged by size: 

                 487     144      32
 
MspA1I CmG'CkG

Cuts at:      0     123     663 
   Size:        123     540
 
NotI GC'GGCC_GC

Cuts at:      0      49     663 
   Size:         49     614
 
PstI C_TGCA'G

Cuts at:      0      80     128     663 
   Size:         80      48     535

  Fragments arranged by size: 

                 535      80      48
 
PvuII CAG'CTG

Cuts at:      0     123     663 
   Size:        123     540
 
SfcI C'TryA_G

Cuts at:      0      76     124     663 
   Size:         76      48     539

  Fragments arranged by size: 

                 539      76      48
 
SmlI C'TyrA_G

Cuts at:      0     133     663 
   Size:        133     530
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     130     162     616     648     663 
   Size:        130      32     454      32      15

  Fragments arranged by size: 

                 454     130      32      32      15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     246     663 
   Size:        246     417
 
ZraI GAC'GTC

Cuts at:      0      85     663 
   Size:         85     578
 

 Enzymes that do cut:

    AatII     AcuI    AlwNI     ApoI     BaeI     BcgI    BciVI    BmgBI
     BmrI     BpmI    BpuEI    BsaHI    BseRI    BsiEI     BsmI    BsrDI
   BssHII   BstAPI   BstEII    BstYI     BtsI     EaeI     EagI  Eco57MI
    Hin4I   MspA1I     NotI     PstI    PvuII     SfcI     SmlI     TstI
     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI
     BsgI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI    BssSI    BstBI    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI
     XmaI     XmnI