(Linear) (Six-base) MAPSORT of: KIEE6251.seq  Check: 9700  from: 1  to: 792



KIEE6251.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:28  ..

AflIII A'CryG_T

Cuts at:      0     390     792 
   Size:        390     402
 
AleI CACnn'nnGTG

Cuts at:      0     152     792 
   Size:        152     640
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     216     792 
   Size:        216     576
 
ApaI G_GGCC'C

Cuts at:      0     649     792 
   Size:        649     143
 
ApoI r'AATT_y

Cuts at:      0     301     792 
   Size:        301     491
 
BanII G_rGCy'C

Cuts at:      0     649     792 
   Size:        649     143
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     660     792 
   Size:        660     132
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     521     555     792 
   Size:        521      34     237

  Fragments arranged by size: 

                 521     237      34
 
BglII A'GATC_T

Cuts at:      0     430     792 
   Size:        430     362
 
Bme1580I G_kGCm'C

Cuts at:      0     649     792 
   Size:        649     143
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     707     792 
   Size:        310     397      85

  Fragments arranged by size: 

                 397     310      85
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300     792 
   Size:        300     492
 
BseYI C'CCAG_C

Cuts at:      0     458     792 
   Size:        458     334
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     617     792 
   Size:        617     175
 
Bsp1286I G_dGCh'C

Cuts at:      0     649     792 
   Size:        649     143
 
BspHI T'CATG_A

Cuts at:      0     693     792 
   Size:        693      99
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     604     792 
   Size:        604     188
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     588     792 
   Size:        588     204
 
BstYI r'GATC_y

Cuts at:      0     330     412     430     792 
   Size:        330      82      18     362

  Fragments arranged by size: 

                 362     330      82      18
 
BtsI GCAGTG_nn'

Cuts at:      0     198     792 
   Size:        198     594
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     155     792 
   Size:        155     637
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     707     792 
   Size:        310     397      85

  Fragments arranged by size: 

                 397     310      85
 
EcoO109I rG'GnC_Cy

Cuts at:      0     645     646     792 
   Size:        645       1     146

  Fragments arranged by size: 

                 645     146       1
 
EcoRV GAT'ATC

Cuts at:      0     348     792 
   Size:        348     444
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     322     354     792 
   Size:        322      32     438

  Fragments arranged by size: 

                 438     322      32
 
HincII GTy'rAC

Cuts at:      0     737     792 
   Size:        737      55
 
HindIII A'AGCT_T

Cuts at:      0     361     792 
   Size:        361     431
 
HpaI GTT'AAC

Cuts at:      0     737     792 
   Size:        737      55
 
MslI CAynn'nnrTG

Cuts at:      0     152     698     792 
   Size:        152     546      94

  Fragments arranged by size: 

                 546     152      94
 
NspI r_CATG'y

Cuts at:      0     394     792 
   Size:        394     398
 
PciI A'CATG_T

Cuts at:      0     390     792 
   Size:        390     402
 
PspOMI G'GGCC_C

Cuts at:      0     645     792 
   Size:        645     147
 
SfcI C'TryA_G

Cuts at:      0     677     792 
   Size:        677     115
 
SmlI C'TyrA_G

Cuts at:      0     315     792 
   Size:        315     477
 
StuI AGG'CCT

Cuts at:      0     274     792 
   Size:        274     518
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     171     792 
   Size:        171     621
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     397     429     792 
   Size:        397      32     363

  Fragments arranged by size: 

                 397     363      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     589     792 
   Size:        589     203
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     117     285     792 
   Size:        117     168     507

  Fragments arranged by size: 

                 507     168     117
 

 Enzymes that do cut:

   AflIII     AleI     AlfI     ApaI     ApoI    BanII     BbsI     BcgI
    BglII Bme1580I     BpmI    BpuEI    BseYI     BsgI Bsp1286I    BspHI
   BstAPI    BstXI    BstYI     BtsI     EciI  Eco57MI EcoO109I    EcoRV
    Hin4I   HincII  HindIII     HpaI     MslI     NspI     PciI   PspOMI
     SfcI     SmlI     StuI    TaqII     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI  EcoICRI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI    HaeII     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI
     XbaI     XhoI     XmaI     XmnI     ZraI