Restriction map for
pFN21AE6245
(Linear) (Six-base) MAPSORT of: KIEE6245.seq Check: 4148 from: 1 to: 783
KIEE6245.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 694 783
Size: 436 258 89
Fragments arranged by size:
436 258 89
AleI CACnn'nnGTG
Cuts at: 0 355 783
Size: 355 428
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 370 402 783
Size: 370 32 381
Fragments arranged by size:
381 370 32
AlwNI CAG_nnn'CTG
Cuts at: 0 234 282 549 672 720 783
Size: 234 48 267 123 48 63
Fragments arranged by size:
267 234 123 63 48 48
AvaI C'yCGr_G
Cuts at: 0 626 783
Size: 626 157
BanI G'GyrC_C
Cuts at: 0 163 195 211 241 783
Size: 163 32 16 30 542
Fragments arranged by size:
542 163 32 30 16
BanII G_rGCy'C
Cuts at: 0 257 307 762 783
Size: 257 50 455 21
Fragments arranged by size:
455 257 50 21
BbeI G_GCGC'C
Cuts at: 0 167 215 245 783
Size: 167 48 30 538
Fragments arranged by size:
538 167 48 30
BglI GCCn_nnn'nGGC
Cuts at: 0 270 783
Size: 270 513
BlpI GC'TnA_GC
Cuts at: 0 724 783
Size: 724 59
Bme1580I G_kGCm'C
Cuts at: 0 200 375 480 783
Size: 200 175 105 303
Fragments arranged by size:
303 200 175 105
BmrI ACTGGGnnnn_n'
Cuts at: 0 378 783
Size: 378 405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 577 776 783
Size: 169 408 199 7
Fragments arranged by size:
408 199 169 7
Bpu10I CC'TnA_GC
Cuts at: 0 115 783
Size: 115 668
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 783
Size: 338 445
BsaI GGTCTCn'nnnn_
Cuts at: 0 625 783
Size: 625 158
BsaAI yAC'GTr
Cuts at: 0 765 783
Size: 765 18
BsaHI Gr'CG_yC
Cuts at: 0 164 212 242 783
Size: 164 48 30 541
Fragments arranged by size:
541 164 48 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 370 400 749 779 783
Size: 370 30 349 30 4
Fragments arranged by size:
370 349 30 30 4
BseYI C'CCAG_C
Cuts at: 0 12 159 497 783
Size: 12 147 338 286
Fragments arranged by size:
338 286 147 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 783
Size: 533 250
BsiEI CG_ry'CG
Cuts at: 0 60 783
Size: 60 723
BsiHKAI G_wGCw'C
Cuts at: 0 257 783
Size: 257 526
Bsp1286I G_dGCh'C
Cuts at: 0 200 257 307 375 480 762 783
Size: 200 57 50 68 105 282 21
Fragments arranged by size:
282 200 105 68 57 50 21
BsrBI CCG'CTC
Cuts at: 0 75 171 783
Size: 75 96 612
Fragments arranged by size:
612 96 75
BsrFI r'CCGG_y
Cuts at: 0 220 783
Size: 220 563
BssSI C'ACGA_G
Cuts at: 0 527 783
Size: 527 256
BstAPI GCAn_nnn'nTGC
Cuts at: 0 234 282 783
Size: 234 48 501
Fragments arranged by size:
501 234 48
BtgI C'CryG_G
Cuts at: 0 48 452 638 783
Size: 48 404 186 145
Fragments arranged by size:
404 186 145 48
BtsI GCAGTG_nn'
Cuts at: 0 136 783
Size: 136 647
EaeI y'GGCC_r
Cuts at: 0 57 783
Size: 57 726
EagI C'GGCC_G
Cuts at: 0 57 783
Size: 57 726
EcoICRI GAG'CTC
Cuts at: 0 255 783
Size: 255 528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 436 577 694 776 783
Size: 169 267 141 117 82 7
Fragments arranged by size:
267 169 141 117 82 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 491 783
Size: 491 292
EcoO109I rG'GnC_Cy
Cuts at: 0 41 546 595 783
Size: 41 505 49 188
Fragments arranged by size:
505 188 49 41
HaeII r_GCGC'y
Cuts at: 0 122 167 215 245 505 556 783
Size: 122 45 48 30 260 51 227
Fragments arranged by size:
260 227 122 51 48 45 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 736 768 783
Size: 736 32 15
Fragments arranged by size:
736 32 15
HincII GTy'rAC
Cuts at: 0 459 783
Size: 459 324
HpaI GTT'AAC
Cuts at: 0 459 783
Size: 459 324
KasI G'GCGC_C
Cuts at: 0 163 211 241 783
Size: 163 48 30 542
Fragments arranged by size:
542 163 48 30
MslI CAynn'nnrTG
Cuts at: 0 355 610 783
Size: 355 255 173
Fragments arranged by size:
355 255 173
MspA1I CmG'CkG
Cuts at: 0 50 147 234 783
Size: 50 97 87 549
Fragments arranged by size:
549 97 87 50
NaeI GCC'GGC
Cuts at: 0 222 783
Size: 222 561
NarI GG'CG_CC
Cuts at: 0 164 212 242 783
Size: 164 48 30 541
Fragments arranged by size:
541 164 48 30
NgoMIV G'CCGG_C
Cuts at: 0 220 783
Size: 220 563
NspI r_CATG'y
Cuts at: 0 615 654 783
Size: 615 39 129
Fragments arranged by size:
615 129 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 549 783
Size: 549 234
PfoI T'CCnGG_A
Cuts at: 0 425 783
Size: 425 358
PmlI CAC'GTG
Cuts at: 0 765 783
Size: 765 18
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 370 402 783
Size: 370 32 381
Fragments arranged by size:
381 370 32
PpuMI rG'GwC_Cy
Cuts at: 0 546 595 783
Size: 546 49 188
Fragments arranged by size:
546 188 49
PstI C_TGCA'G
Cuts at: 0 278 668 783
Size: 278 390 115
Fragments arranged by size:
390 278 115
PvuII CAG'CTG
Cuts at: 0 147 234 783
Size: 147 87 549
Fragments arranged by size:
549 147 87
SacI G_AGCT'C
Cuts at: 0 257 783
Size: 257 526
SacII CC_GC'GG
Cuts at: 0 51 783
Size: 51 732
SfcI C'TryA_G
Cuts at: 0 274 664 783
Size: 274 390 119
Fragments arranged by size:
390 274 119
SfoI GGC'GCC
Cuts at: 0 165 213 243 783
Size: 165 48 30 540
Fragments arranged by size:
540 165 48 30
SmaI CCC'GGG
Cuts at: 0 628 783
Size: 628 155
SmlI C'TyrA_G
Cuts at: 0 353 783
Size: 353 430
StuI AGG'CCT
Cuts at: 0 421 783
Size: 421 362
StyI C'CwwG_G
Cuts at: 0 266 783
Size: 266 517
TatI w'GTAC_w
Cuts at: 0 506 783
Size: 506 277
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 749 781 783
Size: 749 32 2
Fragments arranged by size:
749 32 2
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 330 783
Size: 330 453
XmaI C'CCGG_G
Cuts at: 0 626 783
Size: 626 157
XmnI GAAnn'nnTTC
Cuts at: 0 421 783
Size: 421 362
Enzymes that do cut:
AcuI AleI AloI AlwNI AvaI BanI BanII BbeI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BsrBI BsrFI BssSI BstAPI BtgI BtsI EaeI EagI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HpaI
KasI MslI MspA1I NaeI NarI NgoMIV NspI PflMI
PfoI PmlI PpiI PpuMI PstI PvuII SacI SacII
SfcI SfoI SmaI SmlI StuI StyI TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BmgBI BmtI BplI BsaBI BsaWI
BseRI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrGI BssHII BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI
EcoRI EcoRV FalI FseI FspI FspAI HindIII KpnI
MfeI MluI MmeI MscI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TaqII Tth111I XbaI XhoI
ZraI