Restriction map for
pFN21AE6244
(Linear) (Six-base) MAPSORT of: KIEE6244.seq Check: 3572 from: 1 to: 834
KIEE6244.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 666 748 834
Size: 666 82 86
Fragments arranged by size:
666 86 82
AatII G_ACGT'C
Cuts at: 0 722 834
Size: 722 112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 834
Size: 641 193
AfeI AGC'GCT
Cuts at: 0 340 433 834
Size: 340 93 401
Fragments arranged by size:
401 340 93
AlwNI CAG_nnn'CTG
Cuts at: 0 681 834
Size: 681 153
ApaI G_GGCC'C
Cuts at: 0 383 834
Size: 383 451
AvaI C'yCGr_G
Cuts at: 0 403 511 834
Size: 403 108 323
Fragments arranged by size:
403 323 108
BanI G'GyrC_C
Cuts at: 0 559 588 834
Size: 559 29 246
Fragments arranged by size:
559 246 29
BanII G_rGCy'C
Cuts at: 0 227 383 834
Size: 227 156 451
Fragments arranged by size:
451 227 156
BbeI G_GCGC'C
Cuts at: 0 563 834
Size: 563 271
BbsI GAAGACnn'nnnn_
Cuts at: 0 405 834
Size: 405 429
BglI GCCn_nnn'nGGC
Cuts at: 0 158 834
Size: 158 676
BlpI GC'TnA_GC
Cuts at: 0 58 834
Size: 58 776
Bme1580I G_kGCm'C
Cuts at: 0 383 593 834
Size: 383 210 241
Fragments arranged by size:
383 241 210
BsaHI Gr'CG_yC
Cuts at: 0 410 560 719 834
Size: 410 150 159 115
Fragments arranged by size:
410 159 150 115
BsaWI w'CCGG_w
Cuts at: 0 333 834
Size: 333 501
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 612 834
Size: 612 222
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 367 694 834
Size: 73 294 327 140
Fragments arranged by size:
327 294 140 73
BsiEI CG_ry'CG
Cuts at: 0 71 162 834
Size: 71 91 672
Fragments arranged by size:
672 91 71
BsmBI CGTCTCn'nnnn_
Cuts at: 0 288 834
Size: 288 546
Bsp1286I G_dGCh'C
Cuts at: 0 227 383 593 834
Size: 227 156 210 241
Fragments arranged by size:
241 227 210 156
BspEI T'CCGG_A
Cuts at: 0 333 834
Size: 333 501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 548 666 748 834
Size: 548 118 82 86
Fragments arranged by size:
548 118 86 82
BsrFI r'CCGG_y
Cuts at: 0 425 825 834
Size: 425 400 9
Fragments arranged by size:
425 400 9
BssHII G'CGCG_C
Cuts at: 0 101 834
Size: 101 733
BssSI C'ACGA_G
Cuts at: 0 730 834
Size: 730 104
BstYI r'GATC_y
Cuts at: 0 503 834
Size: 503 331
BtgI C'CryG_G
Cuts at: 0 23 125 153 383 602 834
Size: 23 102 28 230 219 232
Fragments arranged by size:
232 230 219 102 28 23
BtsI GCAGTG_nn'
Cuts at: 0 440 707 803 834
Size: 440 267 96 31
Fragments arranged by size:
440 267 96 31
EaeI y'GGCC_r
Cuts at: 0 10 20 68 159 458 834
Size: 10 10 48 91 299 376
Fragments arranged by size:
376 299 91 48 10 10
EagI C'GGCC_G
Cuts at: 0 68 159 834
Size: 68 91 675
Fragments arranged by size:
675 91 68
EarI CTCTTCn'nnn_
Cuts at: 0 376 618 834
Size: 376 242 216
Fragments arranged by size:
376 242 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 111 730 834
Size: 111 619 104
Fragments arranged by size:
619 111 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 641 834
Size: 641 193
EcoNI CCTnn'n_nnAGG
Cuts at: 0 322 834
Size: 322 512
EcoO109I rG'GnC_Cy
Cuts at: 0 243 327 379 537 834
Size: 243 84 52 158 297
Fragments arranged by size:
297 243 158 84 52
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 579 666 834
Size: 579 87 168
Fragments arranged by size:
579 168 87
HaeII r_GCGC'y
Cuts at: 0 31 342 435 563 834
Size: 31 311 93 128 271
Fragments arranged by size:
311 271 128 93 31
HindIII A'AGCT_T
Cuts at: 0 204 834
Size: 204 630
KasI G'GCGC_C
Cuts at: 0 559 834
Size: 559 275
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 834
Size: 311 523
MslI CAynn'nnrTG
Cuts at: 0 445 451 834
Size: 445 6 383
Fragments arranged by size:
445 383 6
MspA1I CmG'CkG
Cuts at: 0 25 57 701 834
Size: 25 32 644 133
Fragments arranged by size:
644 133 32 25
NaeI GCC'GGC
Cuts at: 0 827 834
Size: 827 7
NarI GG'CG_CC
Cuts at: 0 560 834
Size: 560 274
NgoMIV G'CCGG_C
Cuts at: 0 825 834
Size: 825 9
PfoI T'CCnGG_A
Cuts at: 0 302 834
Size: 302 532
PpuMI rG'GwC_Cy
Cuts at: 0 243 327 537 834
Size: 243 84 210 297
Fragments arranged by size:
297 243 210 84
PspOMI G'GGCC_C
Cuts at: 0 379 834
Size: 379 455
PstI C_TGCA'G
Cuts at: 0 260 834
Size: 260 574
PvuII CAG'CTG
Cuts at: 0 57 834
Size: 57 777
SacII CC_GC'GG
Cuts at: 0 26 834
Size: 26 808
SanDI GG'GwC_CC
Cuts at: 0 243 834
Size: 243 591
SapI GCTCTTCn'nnn_
Cuts at: 0 376 834
Size: 376 458
ScaI AGT'ACT
Cuts at: 0 214 834
Size: 214 620
SfcI C'TryA_G
Cuts at: 0 256 834
Size: 256 578
SfoI GGC'GCC
Cuts at: 0 561 834
Size: 561 273
StyI C'CwwG_G
Cuts at: 0 83 834
Size: 83 751
TatI w'GTAC_w
Cuts at: 0 95 212 608 834
Size: 95 117 396 226
Fragments arranged by size:
396 226 117 95
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 453 600 834
Size: 453 147 234
Fragments arranged by size:
453 234 147
XmnI GAAnn'nnTTC
Cuts at: 0 646 649 834
Size: 646 3 185
Fragments arranged by size:
646 185 3
ZraI GAC'GTC
Cuts at: 0 720 834
Size: 720 114
Enzymes that do cut:
AarI AatII AcuI AfeI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BglI BlpI Bme1580I BsaHI BsaWI
BseRI BsgI BsiEI BsmBI Bsp1286I BspEI BspMI BsrFI
BssHII BssSI BstYI BtgI BtsI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I FalI HaeII HindIII KasI
MmeI MslI MspA1I NaeI NarI NgoMIV PfoI PpuMI
PspOMI PstI PvuII SacII SanDI SapI ScaI SfcI
SfoI StyI TatI XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaXI BseYI BsiHKAI BsiWI BsmI BspHI
BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI
EcoRI EcoRV FseI FspI FspAI Hin4I HincII HpaI
KpnI MfeI MluI MscI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SbfI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XhoI XmaI