Restriction map for
pFN21AE6237
(Linear) (Six-base) MAPSORT of: KIEE6237.seq Check: 6212 from: 1 to: 720
KIEE6237.orf
With 182 enzymes: SgfI *
March 16, 2015 11:28 ..
Acc65I G'GTAC_C
Cuts at: 0 473 720
Size: 473 247
AflIII A'CryG_T
Cuts at: 0 110 720
Size: 110 610
AlwNI CAG_nnn'CTG
Cuts at: 0 467 720
Size: 467 253
ApaI G_GGCC'C
Cuts at: 0 389 508 644 720
Size: 389 119 136 76
Fragments arranged by size:
389 136 119 76
ApaLI G'TGCA_C
Cuts at: 0 343 720
Size: 343 377
AvaI C'yCGr_G
Cuts at: 0 227 426 643 720
Size: 227 199 217 77
Fragments arranged by size:
227 217 199 77
BamHI G'GATC_C
Cuts at: 0 101 720
Size: 101 619
BanI G'GyrC_C
Cuts at: 0 40 473 720
Size: 40 433 247
Fragments arranged by size:
433 247 40
BanII G_rGCy'C
Cuts at: 0 80 389 508 644 650 720
Size: 80 309 119 136 6 70
Fragments arranged by size:
309 136 119 80 70 6
BglI GCCn_nnn'nGGC
Cuts at: 0 487 569 720
Size: 487 82 151
Fragments arranged by size:
487 151 82
BglII A'GATC_T
Cuts at: 0 131 720
Size: 131 589
Bme1580I G_kGCm'C
Cuts at: 0 347 389 508 644 720
Size: 347 42 119 136 76
Fragments arranged by size:
347 136 119 76 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 99 720
Size: 99 621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 316 489 720
Size: 309 7 173 231
Fragments arranged by size:
309 231 173 7
Bpu10I CC'TnA_GC
Cuts at: 0 361 720
Size: 361 359
BsaAI yAC'GTr
Cuts at: 0 342 720
Size: 342 378
BsaHI Gr'CG_yC
Cuts at: 0 50 215 239 281 720
Size: 50 165 24 42 439
Fragments arranged by size:
439 165 50 42 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 720
Size: 214 506
BsiEI CG_ry'CG
Cuts at: 0 311 720
Size: 311 409
BsiHKAI G_wGCw'C
Cuts at: 0 347 674 720
Size: 347 327 46
Fragments arranged by size:
347 327 46
Bsp1286I G_dGCh'C
Cuts at: 0 80 347 389 508 644 650 674 720
Size: 80 267 42 119 136 6 24 46
Fragments arranged by size:
267 136 119 80 46 42 24 6
BsrFI r'CCGG_y
Cuts at: 0 37 235 481 605 720
Size: 37 198 246 124 115
Fragments arranged by size:
246 198 124 115 37
BstAPI GCAn_nnn'nTGC
Cuts at: 0 40 720
Size: 40 680
BstEII G'GTnAC_C
Cuts at: 0 231 720
Size: 231 489
BstYI r'GATC_y
Cuts at: 0 101 131 720
Size: 101 30 589
Fragments arranged by size:
589 101 30
BtgI C'CryG_G
Cuts at: 0 530 564 720
Size: 530 34 156
Fragments arranged by size:
530 156 34
EaeI y'GGCC_r
Cuts at: 0 203 404 488 720
Size: 203 201 84 232
Fragments arranged by size:
232 203 201 84
EarI CTCTTCn'nnn_
Cuts at: 0 253 720
Size: 253 467
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 109 720
Size: 88 21 611
Fragments arranged by size:
611 88 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 309 316 489 720
Size: 309 7 173 231
Fragments arranged by size:
309 231 173 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 364 720
Size: 364 356
EcoO109I rG'GnC_Cy
Cuts at: 0 357 386 720
Size: 357 29 334
Fragments arranged by size:
357 334 29
HaeII r_GCGC'y
Cuts at: 0 10 660 720
Size: 10 650 60
Fragments arranged by size:
650 60 10
KpnI G_GTAC'C
Cuts at: 0 477 720
Size: 477 243
MslI CAynn'nnrTG
Cuts at: 0 340 720
Size: 340 380
MspA1I CmG'CkG
Cuts at: 0 153 402 566 720
Size: 153 249 164 154
Fragments arranged by size:
249 164 154 153
NaeI GCC'GGC
Cuts at: 0 483 607 720
Size: 483 124 113
Fragments arranged by size:
483 124 113
NgoMIV G'CCGG_C
Cuts at: 0 481 605 720
Size: 481 124 115
Fragments arranged by size:
481 124 115
NspI r_CATG'y
Cuts at: 0 114 720
Size: 114 606
PciI A'CATG_T
Cuts at: 0 110 720
Size: 110 610
PmlI CAC'GTG
Cuts at: 0 342 720
Size: 342 378
PpuMI rG'GwC_Cy
Cuts at: 0 357 720
Size: 357 363
PshAI GACnn'nnGTC
Cuts at: 0 238 720
Size: 238 482
PspOMI G'GGCC_C
Cuts at: 0 385 504 640 720
Size: 385 119 136 80
Fragments arranged by size:
385 136 119 80
RsrII CG'GwC_CG
Cuts at: 0 450 720
Size: 450 270
SacII CC_GC'GG
Cuts at: 0 567 720
Size: 567 153
SmaI CCC'GGG
Cuts at: 0 229 428 645 720
Size: 229 199 217 75
Fragments arranged by size:
229 217 199 75
SrfI GCCC'GGGC
Cuts at: 0 645 720
Size: 645 75
StyI C'CwwG_G
Cuts at: 0 119 720
Size: 119 601
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 325 720
Size: 325 395
XmaI C'CCGG_G
Cuts at: 0 227 426 643 720
Size: 227 199 217 77
Fragments arranged by size:
227 217 199 77
Enzymes that do cut:
Acc65I AflIII AlwNI ApaI ApaLI AvaI BamHI BanI
BanII BglI BglII Bme1580I BplI BpmI Bpu10I BsaAI
BsaHI BsgI BsiEI BsiHKAI Bsp1286I BsrFI BstAPI BstEII
BstYI BtgI EaeI EarI EciI Eco57MI EcoNI EcoO109I
HaeII KpnI MslI MspA1I NaeI NgoMIV NspI PciI
PmlI PpuMI PshAI PspOMI RsrII SacII SmaI SrfI
StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BlpI BmgBI BmrI BmtI BpuEI BsaI BsaBI
BsaWI BsaXI BseRI BseYI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI MfeI
MluI MmeI MscI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PpiI
PsiI PspXI PsrI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI